Lipopolysaccharide‐induced expression of FAS ligand in cultured immature boar sertoli cells through the regulation of pro‐inflammatory cytokines and <i>miR‐187</i>

Wang, Yi; Zhang, Jiaojiao; Yang, Wenjing; Luo, Hongyan; Zhang, Jiahua; Wang, Xianzhong

doi:10.1002/mrd.22534

Cited by 7 publications

(3 citation statements)

References 60 publications

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“…On one hand, proinflammatory cytokines such as tumor necrosis factor-alpha (TNF-α), interleukin-1B (IL-1B), nitric oxide (NO), and transforming growth factor-beta (TGF-β) were elevated when lipopolysaccharide (LPS) were added to immature cultured porcine Sertoli cells. FasL expression levels were increased and cell viability decreased in a time-and dose-dependent manner (Wang et al 2015). It is well known that aromatase converts androgen into estrogen, which promotes FasL expression through estrogen receptor alpha (ERα) and Sp-1 protein (Cooke et al 2017).…”

Section: Regulation Of the Fas/fasl Signaling Pathway In The Sertoli mentioning

confidence: 99%

The role of the Fas/FasL signaling pathway in environmental toxicant-induced testicular cell apoptosis: An update

Wang

2018

Systems Biology in Reproductive Medicine

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BTB: blood-testis barrier; DD: death domains; DR3: death receptor 3; DR4: death receptor 4; DR5: death receptor 5; DED: death effector domain; DISC: death-inducing signaling complex; ERα: estrogen receptor alpha; FADD: Fas-associated death domain; FSH: follicle- stimulating hormone; IL-1β: interleukin 1 beta; LH: luteinizing hormone; LPS: lipopolysaccharide; mFas: membrane Fas; MMP2: matrix metalloproteinase-2; MTA1: metastasis-associated protein 1; NAC: N-acetylcysteine; NCCD: the Nomenclature Committee on Cell Death; NFAT: nuclear factor of activated T-cells; NF-kB: nuclear transcription factor-kappaB; NO: nitric oxide; NP: 4-nonylphenol; PCD: programmed cell death; PP1/PP2A: protein phosphatase 1 and 2A; ROS: reactive oxygen species; sFas: soluble Fas; T: testosterone; TGF-β: transforming growth factor-beta; THD: TNF homology domain; TIMP-2: tissue inhibitor of metalloproteinase-2; TNF: tumor necrosis factor; TNF-α: tumor necrosis factor-alpha; TNF-R1: Tumor necrosis factor receptor 1; TNFRSF1A: TNF receptor superfamily member 1A.

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Section: Regulation Of the Fas/fasl Signaling Pathway In The Sertoli mentioning

confidence: 99%

The role of the Fas/FasL signaling pathway in environmental toxicant-induced testicular cell apoptosis: An update

Wang

2018

Systems Biology in Reproductive Medicine

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“…Sertoli cell isolation and culture were performed according to previous procedures in our laboratory (Wang et al 2015). In brief, testes were collected in a sterile manner from immature boars housed at a local farm.…”

Section: Sertoli Cell Isolation and Culturementioning

confidence: 99%

ERK1/2 regulates heat stress-induced lactate production via enhancing the expression of HSP70 in immature boar Sertoli cells

Guan

Liao

et al. 2018

Cell Stress and Chaperones

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Lactate produced by Sertoli cells plays an important role in spermatogenesis, and heat stress induces lactate production in immature boar Sertoli cells. Extracellular signaling regulated kinase 1 and 2 (ERK1/2) participates in heat stress response. However, the effect of ERK1/2 on heat stress-induced lactate production is unclear. In the present study, Sertoli cells were isolated from immature boar testis and cultured at 32 °C. Heat stress was induced in a 43 °C incubator for 30 min. Proteins and RNAs were detected by western blotting and RT-PCR, respectively. Lactate production and lactate dehydrogenase (LDH) activity were detected using commercial kits. Heat stress promoted ERK1/2 phosphorylation, showing a reducing trend with increasing recovery time. In addition, heat stress increased heat shock protein 70 (HSP70), glucose transporter 3 (GLUT3), and lactate dehydrogenase A (LDHA) expressions, enhanced LDH activity and lactate production at 2-h post-heat stress. Pretreatment with U0126 (1 × 10 mol/L), a highly selective inhibitor of ERK1/2 phosphorylation, reduced HSP70, GLUT3, and LDHA expressions and decreased LDH activity and lactate production. Meanwhile, ERK2 siRNA1 reduced the mRNA level of ERK2 and weakened ERK1/2 phosphorylation. Additionally, ERK2 siRNA1 reduced HSP70, GLUT3, and LHDA expressions decreased LDH activity and lactate production. Furthermore, HSP70 siRNA3 downregulated GLUT3 and LDHA expressions and decreased LDH activity and lactate production. These results show that activated ERK1/2 increases heat stress-induced lactate production by enhancing HSP70 expression to promote the expressions of molecules related to lactate production (GLUT3 and LDHA). Our study reveals a new insight in reducing the negative effect of heat stress in boars.

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“…LPS that is known to induce the inflammatory response was reported to reduce the growth of SCs [ 30 ]. To determine the effect of LPS treatment on expression of ssc-miR-149 and TRAF3 , the SCs were treated with 10 μg/mL LPS as previously reported [ 30 ]. The result showed that LPS could induce ssc-miR-149 expression in a time dependent manner (Fig.…”

Section: Resultsmentioning

confidence: 99%

Profiling of miRNAs in porcine Sertoli cells

Chen

Zheng

et al. 2020

J Animal Sci Biotechnol

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Background: Sertoli cells (SCs) create a specialized environment to support and dictate spermatogenesis. MicroRNAs (miRNAs), a kind of~22 nt small noncoding RNAs, have been reported to be highly abundant in mouse SCs and play critical roles in spermatogenesis. However, the miRNAs of porcine SCs remain largely unknown. Methods: We isolated porcine SCs and conducted small RNA sequencing. By comparing miRNAs in germ cells, we systematically analyzed the miRNA expression pattern of porcine SCs. We screened the highly enriched SC miRNAs and predicted their functions by Gene Ontology analysis. The dual luciferase assay was used to elucidate the regulation of tumor necrosis factor receptor (TNFR)-associated factor 3 (TRAF3) by ssc-miR-149. Results: The analysis showed that 18 miRNAs were highly expressed in SCs and 15 miRNAs were highly expressed in germ cells. These miRNAs were predicted to mediate SC and germ cell functions. In addition, ssc-miR-149 played critical roles in SCs by targeting TRAF3. Conclusion: Our findings provide novel insights into the miRNA expression pattern and their regulatory roles of porcine SCs.

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Lipopolysaccharide‐induced expression of FAS ligand in cultured immature boar sertoli cells through the regulation of pro‐inflammatory cytokines and miR‐187

Cited by 7 publications

References 60 publications

The role of the Fas/FasL signaling pathway in environmental toxicant-induced testicular cell apoptosis: An update

The role of the Fas/FasL signaling pathway in environmental toxicant-induced testicular cell apoptosis: An update

ERK1/2 regulates heat stress-induced lactate production via enhancing the expression of HSP70 in immature boar Sertoli cells

Profiling of miRNAs in porcine Sertoli cells

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