Profiling of miRNAs in porcine Sertoli cells

Chen, Xiaoxu; Zheng, Yi; Li, Xueliang; Gao, Qiang; Feng, Tongying; Zhang, Pengfei; Liao, Mingzhi; Tian, Xiue; Lu, Hongzhao

doi:10.1186/s40104-020-00487-6

Cited by 11 publications

(7 citation statements)

References 57 publications

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“…RNA-seq data investigation shows that miR-24-3p exhibits a developmentalstage-specific expression profile in porcine testicular tissues [33,34]. In addition, miR-24-3p is also cloned from the mice Sertoli cells [35], and it also shows a higher expression in porcine Sertoli cells than in other spermatogenic cell types [36]. However, there is no evidence to explore the regulatory role of miR-24-3p in porcine Sertoli cell proliferation.…”

Section: Discussionmentioning

confidence: 99%

circBTBD7 Promotes Immature Porcine Sertoli Cell Growth through Modulating miR-24-3p/MAPK7 Axis to Inactivate p38 MAPK Signaling Pathway

Bian

Chen

Wang

et al. 2021

IJMS

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Sertoli cells are the crucial coordinators to guarantee normal spermatogenesis and male fertility. Although circular RNAs (circRNAs) exhibit developmental-stage-specific expression in porcine testicular tissues and have been thought of as potential regulatory molecules in spermatogenesis, their functions and mechanisms of action remain largely unknown, especially in domestic animals. A novel circBTBD7 was identified from immature porcine Sertoli cells using reverse transcription PCR, Sanger sequencing, and fluorescence in situ hybridization assays. Functional assays illustrated that circBTBD7 overexpression promoted cell cycle progression and cell proliferation, as well as inhibited cell apoptosis in immature porcine Sertoli cells. Mechanistically, circBTBD7 acted as a sponge for the miR-24-3p and further facilitated its target mitogen-activated protein kinase 7 (MAPK7) gene. Overexpression of miR-24-3p impeded cell proliferation and induced cell apoptosis, which further attenuated the effects of circBTBD7 overexpression. siRNA-induced MAPK7 deficiency resulted in a similar effect to miR-24-3p overexpression, and further offset the effects of miR-24-3p inhibition. Both miR-24-3p overexpression and MAPK7 knockdown upregulated the p38 phosphorylation activity. The SB202190 induced the inhibition of p38 MAPK pathway and caused an opposite effect to that of miR-24-3p overexpression and MAPK7 knockdown. Collectively, circBTBD7 promotes immature porcine Sertoli cell growth through modulating the miR-24-3p/MAPK7 axis to inactivate the p38 MAPK signaling pathway. This study expanded our knowledge of noncoding RNAs in porcine normal spermatogenesis through deciding the fate of Sertoli cells.

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Section: Discussionmentioning

confidence: 99%

circBTBD7 Promotes Immature Porcine Sertoli Cell Growth through Modulating miR-24-3p/MAPK7 Axis to Inactivate p38 MAPK Signaling Pathway

Bian

Chen

Wang

et al. 2021

IJMS

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“…The production of the nutrient has been likened to lactation in mammals, and hence, it has been called crop milk [ 39 ]; meanwhile, the pigeon crop undergoes significant changes to the tissue structure during lactation [ 17 ]. miRNAs have demonstrated to play crucial functions in lipid metabolism, adipose tissue development and immune response [ 40 , 41 , 42 ]. In the present study, we found that over 40% of the small RNAs in pigeon crop were miRNAs; a total of 71 miRNAs were identified as significantly differentially expressed.…”

Section: Discussionmentioning

confidence: 99%

Identification of microRNA-Associated-ceRNA Networks Regulating Crop Milk Production in Pigeon (Columba livia)

et al. 2020

Genes

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Pigeon belongs to altrices. Squab cannot forage independently. Nutrition can only be obtained from crop milk secreted by male and female pigeon. miRNA could regulate many biological events. However, the roles of miRNA and ceRNA in regulating crop milk production are still unknown. In this study, we investigated the miRNAs expression profile of female pigeon crop, explored the potential key genes, and found the regulatory mechanisms of crop milk production. A total of 71 miRNAs were identified differentially expressed significantly. Meanwhile, miR-20b-5p, miR-146b-5p, miR-21-5p, and miR-26b-5p were found to be the key miRNAs regulating lactation. Target genes of these miRNAs participated mainly in cell development; protein and lipid synthesis; and ion signaling processes, such as cell-cell adhesion, epithelial cell morphogenesis, calcium signaling pathway, protein digestion, and absorption. In the ceRNA network, miR-193-5p was located in the central position, and miR-193-5p/CREBRF/LOC110355588, miR-460b-5p/GRHL2/MSTRG.132954, and miR-193-5p/PIK3CD/LOC110355588 regulatory axes were believed to affect lactation. Collectively, our findings enriched the miRNA expression profile of pigeon and provided novel insights into the microRNA-associated-ceRNA networks regulating crop milk production in pigeon.

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“…Data on miRNA in relation to testicular toxicity in minipigs are very limited or absent. However, in pigs, miRNA expression profiles from Sertoli cells have been assessed by sRNA-seq (Chen et al 2020). Mature Sertoli cells were isolated from 5-month-old pigs, and the Sertoli cell RNA was further analyzed by sRNA-seq.…”

Section: Pigs/minipigsmentioning

confidence: 99%

Circulating microRNAs as promising testicular translatable safety biomarkers: current state and future perspectives

et al. 2023

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Drug-induced testicular injury (DITI) is one of the often-observed and challenging safety issues seen during drug development. Semen analysis and circulating hormones currently utilized have significant gaps in their ability to detect testicular damage accurately. In addition, no biomarkers enable a mechanistic understanding of the damage to the different regions of the testis, such as seminiferous tubules, Sertoli, and Leydig cells. MicroRNAs (miRNAs) are a class of non-coding RNAs that modulate gene expression post-transcriptionally and have been indicated to regulate a wide range of biological pathways. Circulating miRNAs can be measured in the body fluids due to tissue-specific cell injury/damage or toxicant exposure. Therefore, these circulating miRNAs have become attractive and promising non-invasive biomarkers for assessing drug-induced testicular injury, with several reports on their use as safety biomarkers for monitoring testicular damage in preclinical species. Leveraging emerging tools such as 'organs-on-chips' that can emulate the human organ's physiological environment and function is starting to enable biomarker discovery, validation, and clinical translation for regulatory qualification and implementation in drug development.

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Profiling of miRNAs in porcine Sertoli cells

Cited by 11 publications

References 57 publications

circBTBD7 Promotes Immature Porcine Sertoli Cell Growth through Modulating miR-24-3p/MAPK7 Axis to Inactivate p38 MAPK Signaling Pathway

circBTBD7 Promotes Immature Porcine Sertoli Cell Growth through Modulating miR-24-3p/MAPK7 Axis to Inactivate p38 MAPK Signaling Pathway

Identification of microRNA-Associated-ceRNA Networks Regulating Crop Milk Production in Pigeon (Columba livia)

Circulating microRNAs as promising testicular translatable safety biomarkers: current state and future perspectives

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