Lipopolysaccharides isolated from Aeromonas salmonicida and Vibrio anguillarum show quantitative but not qualitative differences in inflammatory outcome in Sparus aurata (Gilthead seabream)

Boltaña, Sebastián; Tridico, Rosa; Teles, Mariana; Mackenzie, Simon; Tort, L.

doi:10.1016/j.fsi.2014.06.003

Cited by 20 publications

(13 citation statements)

References 47 publications

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“…This has merit in intensive aquaculture where chronic stress can lead to suppressed disease resistance and growth rate, both contributing to suboptimal production. While this study used LPS to elicit a model immune response, studies utilizing fish-specific bacterin/pathogen may be essential to shed light on the energy repartitioning associated with immune response under aquaculture scenario [50, 51]. Better understanding of the molecular mechanisms involved in stress-immune-growth interactions can benefit stress management and ways to improve energy substrate partitioning to enhance growth and disease resistance in aquaculture.…”

Section: Discussionmentioning

confidence: 99%

Stress-Immune-Growth Interactions: Cortisol Modulates Suppressors of Cytokine Signaling and JAK/STAT Pathway in Rainbow Trout Liver

Philip

Vijayan

2015

PLoS ONE

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Chronic stress is a major factor in the poor growth and immune performance of salmonids in aquaculture. However, the molecular mechanisms linking stress effects to growth and immune dysfunction is poorly understood. The suppressors of cytokine signaling (SOCS), a family of genes involved in the inhibition of JAK/STAT pathway, negatively regulates growth hormone and cytokine signaling, but their role in fish is unclear. Here we tested the hypothesis that cortisol modulation of SOCS gene expression is a key molecular mechanism leading to growth and immune suppression in response to stress in fish. Exposure of rainbow trout (Oncorhynchus mykiss) liver slices to cortisol, mimicking stress level, upregulated SOCS-1 and SOCS-2 mRNA abundance and this response was abolished by the glucocorticoid receptor antagonist mifepristone. Bioinformatics analysis confirmed the presence of putative glucocorticoid response elements in rainbow trout SOCS-1 and SOCS-2 promoters. Prior cortisol treatment suppressed acute growth hormone (GH)-stimulated IGF-1 mRNA abundance in trout liver and this involved a reduction in STAT5 phosphorylation and lower total JAK2 protein expression. Prior cortisol treatment also suppressed lipopolysaccharide (LPS)-induced IL-6 but not IL-8 transcript levels; the former but not the latter cytokine expression is via JAK/STAT phosphorylation. LPS treatment reduced GH signaling, but this was associated with the downregulation of GH receptors and not due to the upregulation of SOCS transcript levels by this endotoxin. Collectively, our results suggest that upregulation of SOCS-1 and SOCS-2 transcript levels by cortisol, and the associated reduction in JAK/STAT signaling pathway, may be a novel mechanism leading to growth reduction and immune suppression during stress in trout.

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Section: Discussionmentioning

confidence: 99%

Stress-Immune-Growth Interactions: Cortisol Modulates Suppressors of Cytokine Signaling and JAK/STAT Pathway in Rainbow Trout Liver

Philip

Vijayan

2015

PLoS ONE

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“…; Yui & Kaattari ; Boltaña et al . ). Fish skin mucus often contains concentrations of antimicrobial substances such as lysozyme or specific peptide components which block bacterial access to the epithelial cellular membrane (Ellis ).…”

Section: Pathology and Virulencementioning

confidence: 99%

A comprehensive review of Vibrio (Listonella) anguillarum: ecology, pathology and prevention

Hickey

Lee

2017

Reviews in Aquaculture

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Vibrio (Listonella) anguillarum is a marine bacteria that is pathogenic to a number of aquatic organisms including several species which are important to the aquaculture industry. Organisms that are infected by V. anguillarum are diagnosed with vibriosis which can be lethal in a matter of days. Vibriosis can be particularly devastating to aquaculture businesses and measures of prevention or treatment are generally quite expensive. Efforts to understand and control V. anguillarum virulence have been of high‐priority among international aquatic research studies. The knowledge that has accumulated as a result of this collective research effort is reviewed in this article.

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“…Additionally, gilthead seabream ( S. aurata ) intraperitoneally injected with purified LPS from A. salmonicida and V. anguillarum demonstrated regulation of pro‐inflammatory cytokine abundance and increased resistance to pathogen challenge (Boltaña et al . ). However, they found that immune responses were concentration dependent and may be related to different contaminants present in the LPS.…”

Section: Administration Methods and Doses Of Bacterial Cellular Compomentioning

confidence: 99%

“…Notably, it has been demonstrated that fish are unsusceptible to the toxic effects of LPS that can cause septic shock in mammals (Boltaña et al . ). Fish lack intracellular mediators and several costimulatory molecules (CD14, LY96 and TICAM2) that are involved in the action of LPS in mammals (Boltaña et al .…”

Section: Types Of Bacterial Cellular Preparations Applied In Fish Aqumentioning

confidence: 97%

“…Fish lack intracellular mediators and several costimulatory molecules (CD14, LY96 and TICAM2) that are involved in the action of LPS in mammals (Boltaña et al . ). Earlier data suggested that fish show a lower TLR4 receptor‐mediated LPS recognition functionality than do mammals, mostly because of differences in TLR4 paralog speciation (Sullivan et al .…”

Section: Types Of Bacterial Cellular Preparations Applied In Fish Aqumentioning

confidence: 97%

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Use of bacterial subcellular components as immunostimulants in fish aquaculture

Giri

Chi

Jun

et al. 2016

Reviews in Aquaculture

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Aquaculture and fisheries products are expected to play a leading part in addressing the food necessities of the increasing human population. However, in intensive aquaculture, fish are exposed to stress conditions that deteriorate their immune systems, leading to increased disease vulnerability. These disease outbreaks have resulted in production losses and represent one of the key constraints in fish farming. Accordingly, aquaculture disease management efforts have concentrated on the establishment of environmental friendly and lasting methods. Recently, several studies have revealed that various bacteria (especially probiotics)‐derived cellular components might be used as promising immunostimulants in fish aquaculture. Such components curtail the risk concomitant with the use of antibiotics, chemotherapeutics, vaccines and probiotics. Among the various health advantages ascribed to bacterial cellular components, the modulation of specific and nonspecific immune responses, cytokine gene expression and anti‐pathogenic activities are most anticipated benefits. Their potential as vaccine adjuvants for fish culture has also been demonstrated. However, as an emerging practice, limited knowledge is available regarding the detailed interactions with fish immune systems. Therefore, the underlying mechanisms involved in fish immune cell activation need to be explored further. In this context, the present article aspires to review the available studies on the use of bacterial cellular components in fish culture and their biological effects on fish such as growth promotion, immunostimulation and anti‐pathogenic activities as well as to evaluate the factors that control the optimal induction of immune responses in fish. In addition, future issues that merit further investigation are suggested.

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Lipopolysaccharides isolated from Aeromonas salmonicida and Vibrio anguillarum show quantitative but not qualitative differences in inflammatory outcome in Sparus aurata (Gilthead seabream)

Cited by 20 publications

References 47 publications

Stress-Immune-Growth Interactions: Cortisol Modulates Suppressors of Cytokine Signaling and JAK/STAT Pathway in Rainbow Trout Liver

Stress-Immune-Growth Interactions: Cortisol Modulates Suppressors of Cytokine Signaling and JAK/STAT Pathway in Rainbow Trout Liver

A comprehensive review of Vibrio (Listonella) anguillarum: ecology, pathology and prevention

Use of bacterial subcellular components as immunostimulants in fish aquaculture

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