Lipopolysaccharides of Oral Anaerobes Associated with Chronic Inflammation: Chemical and Immunomodulating Properties

Hamada, Shigeyuki; Takada, Haruhiko; Ogawa, Tomohiko; Fujiwara, Taku; Mihara, J

doi:10.3109/08830189009056635

Cited by 94 publications

(65 citation statements)

References 95 publications

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“…†P < 0.05 indicates a significant difference between values at weeks 1 and 4 week, according to Wilcoxon's signedranks test. been reported in a rat model (35,36) and in human subjects (2). However, due to the difficulties associated with developing effective animal models and the multifaceted nature of periodontitis, passive immunization therapy against periodontitis has not been studied extensively (37).…”

Section: Discussionmentioning

confidence: 99%

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Effects of egg yolk antibody against Porphyromonas gingivalis gingipains in periodontitis patients

et al. 2007

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Porphyromonas gingivalis gingipains issuspected to be one of the most important causative agents of periodontitis. We postulated that the inhibition of gingipains may reduce the pathogenic nature of P. gingivalis. Anti-P. gingivalis egg yolk antibody (IgY-GP) was isolated from the yolks of hens immunized with purified gingipains. We applied IgY-GP gel subgingivally in periodontitis patients who harbored P. gingivalis in their subgingival flora. Five pairs of contralateral anterior single-rooted teeth were selected. One tooth in each contralateral pair was randomly treated with IgY-GP and subgingival scaling and root planing, whereas the other tooth was treated with SRP alone. The number of P. gingivalis bacteria was assessed by real-time PCR. Bacterial levels were expressed as the percentage of total bacteria. The IgY-GP group had a significant reduction in probing depth. BOP significantly decreased in the IgY-GP group compared to the control group at week 4. The levels of P. gingivalis significantly increased in the control group at week 4, whereas the reduction in the levels of P. gingivalis was sustained in the IgY-GP group. Within the limitations of the present study, IgY-GP was shown to be an effective immunotherapeutic agent in the treatment of periodontitis. (J. Oral Sci. 49,[201][202][203][204][205][206] 2007)

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Section: Discussionmentioning

confidence: 99%

“…gingivalis produces a broad spectrum of virulence factors, lipopolysaccharide (2), fimbriae, hemagglutinin, hemolysin, and proteinase (3)(4)(5)(6). Arg-and Lys-cysteine proteinases (gingipains) are the main endopeptidases produced by P. gingivalis (7).…”

Section: Introductionmentioning

confidence: 99%

Effects of egg yolk antibody against Porphyromonas gingivalis gingipains in periodontitis patients

et al. 2007

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“…intermedia, possess various virulence factors on their cell surfaces, such as LPS [5,6] and ®mbriae [16,17]. The immunobiological activities of these factors have been studied extensively in relation to the pathogenesis of periodontal diseases.…”

Section: Mrna Expression For Il-8 G-csf Gm-csf and Icam-1 By Gingivmentioning

confidence: 99%

“…LPS from BPB has been reported to exhibit unique bioactivities that differ from those of common LPS prepared from Enterobacteriaceae, such as Escherichia coli and Salmonella spp. [5,6]. Immunocompetent cells from animals and human peripheral blood cells were used in the initial studies, and subsequently human ®broblasts derived from periodontal tissues were examined.…”

Section: Introductionmentioning

confidence: 99%

Activation of human gingival epithelial cells by cell-surface components of black-pigmented bacteria: augmentation of production of interleukin-8, granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor and expression of intercellular adhesion molecule 1

Sugiyama¹,

Uehara²,

Iki

et al. 2002

Journal of Medical Microbiology

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“…Among the various cell-surface components of the bacterium, ®mbriae [14,15] and LPS [16,17] have been the most thoroughly studied as possible virulence factors for periodontal diseases. Both components are capable of stimulating gingival ®broblasts.…”

Section: Introductionmentioning

confidence: 99%

Enhancement of hepatocyte growth factor (scatter factor) production by human gingival fibroblasts in culture stimulated with Porphyromonas gingivalis fimbriae

Sugiyama

Ogawa

Daikuhara

et al. 2000

Journal of Medical Microbiology

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Hepatocyte growth factor (HGF), also known as scatter factor (SF), is a motogen, mitogen and morphogen produced by mesenchymal cells that mainly acts on epithelial cells and is involved in osteoclast stimulation. This study examined the possible enhanced production of HGF=SF by human gingival ®broblasts upon stimulation with killed cells of Porphyromonas gingivalis strain 381 and its representative bioactive cellular components, ®mbriae and lipopolysaccharide (LPS). P. gingivalis whole cells enhanced the production of HGF=SF detected by ELISA in culture supernates of the ®broblasts. Fimbriae prepared from P. gingivalis exhibited powerful HGF=SF-inducing activity in a concentration-dependent manner, with peak activity observed at 100±200 ìg=ml. The ®mbriae-induced HGF=SF mRNA expression by the cells was also detected by reverse transcription-PCR. P. gingivalis LPS exhibited weak HGF=SF-inducing activity. The study also examined the HGF=SF-inducing activity of seven synthetic peptides corresponding to the segments of P. gingivalis ®mbrial subunit protein. The peptides of residues 282±301 and 302±321, which exhibited antagonistic effects against P. gingivalis ®mbriae-binding to human gingival ®broblasts in a previous study, showed weak activity, whereas other non-antagonistic peptides showed no signi®cant activity. These ®ndings indicated that P. gingivalis ®mbriae enhanced production of HGF=SF by human gingival ®broblasts, whereas synthetic peptide segments of ®mbrial subunit protein were not suf®cient to exert the activity.

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Lipopolysaccharides of Oral Anaerobes Associated with Chronic Inflammation: Chemical and Immunomodulating Properties

Cited by 94 publications

References 95 publications

Effects of egg yolk antibody against Porphyromonas gingivalis gingipains in periodontitis patients

Effects of egg yolk antibody against Porphyromonas gingivalis gingipains in periodontitis patients

Activation of human gingival epithelial cells by cell-surface components of black-pigmented bacteria: augmentation of production of interleukin-8, granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor and expression of intercellular adhesion molecule 1

Enhancement of hepatocyte growth factor (scatter factor) production by human gingival fibroblasts in culture stimulated with Porphyromonas gingivalis fimbriae

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