Lipoprotein fractionation.

Carlson, Kerstin

doi:10.1136/jcp.s1-5.1.32

Cited by 445 publications

(138 citation statements)

References 10 publications

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“…In contrast, the increase in small chylomicron remnants from baseline to 6 h was not related to plasma NEFA concentrations at 6 h (p = 0. 17) cantly higher fasting plasma concentrations of large TRLs. Insulin has been shown to regulate the production rate of large, but not small, VLDL particles in humans [6].…”

Section: Discussionmentioning

confidence: 99%

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Insulin and non-esterified fatty acid relations to alimentary lipaemia and plasma concentrations of postprandial triglyceride-rich lipoproteins in healthy middle-aged men

et al. 2000

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A cluster of risk factors for Type II (non-insulin-dependent) diabetes mellitus and cardiovascular disease, called the metabolic syndrome, is a common feature of otherwise healthy people living in industrialised countries. Increased visceral adipose tissue depots, insulin resistance with ensuing hyperinsulinaemia and dyslipoproteinaemia including hypertriglyceridaemia, a low HDL cholesterol concentration and a preponderance of small, dense LDL particles are central components of this syndrome [1]. The links between glucose-insulin homeostasis and lipoprotein metabolism have received particular atten- Abstract Aims/hypothesis. Enhanced and prolonged postprandial lipaemia is related to cardiovascular disease but how postprandial lipaemia is regulated is poorly known. We therefore determined the relations of fasting insulin concentrations to fasting and postprandial lipids, lipoproteins and non-esterified fatty acids in middle-aged men. Methods. The subjects, 99 healthy 50-year-old men with an apolipoprotein E3/3 genotype, ate a mixed meal. The apolipoprotein B-48 and B-100 contents were determined in triglyceride-rich lipoproteins as a measure of chylomicron remnant and very low density lipoprotein particle concentrations. Results. Fasting plasma insulin was associated with the triglyceride response to the test meal, independently of body mass index, waist-to-hip circumference ratio, blood glucose and the insulin effect on fasting plasma triglycerides. Exaggerated and prolonged postprandial lipaemia in subjects in the upper quartile of the plasma insulin distribution was largely accounted for by large (Svedberg flotation rate > 60) very low density lipoproteins and chylomicron remnants. Insulin relations to large postprandial triglyceride-rich lipoproteins exclusively reflected the association between plasma insulin and the fasting plasma concentrations of these lipoprotein species, whereas plasma insulin and late postprandial plasma concentrations of small (Svedberg flotation rate 20±60) chylomicron remnants were related, independently of insulin influences on fasting concentrations. Strong positive relations were found between the late increases in large triglyceride-rich lipoproteins and plasma non-esterified fatty acid concentrations after 6 h. Conclusion/interpretation. The degree of insulin sensitivity is a major determinant of postprandial lipaemia in healthy middle-aged men and could add to the regulation of the basal production of large triglyceride-rich lipoproteins. [Diabetologia (2000)

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Section: Discussionmentioning

confidence: 99%

“…Fasting plasma concentrations of cholesterol and triglycerides in VLDL, LDL and HDL were determined by a combination of preparative ultracentrifugation, precipitation of apo B-containing lipoproteins and lipid analyses [17]. Triglyceride-rich lipoproteins were subfractionated by cumulative density gradient ultracentrifugation [18].…”

Section: Methodsmentioning

confidence: 99%

Insulin and non-esterified fatty acid relations to alimentary lipaemia and plasma concentrations of postprandial triglyceride-rich lipoproteins in healthy middle-aged men

et al. 2000

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“…Plasma was recovered by means of low-speed centrifugation at 1400 g for 20 min at 1 C and kept at this temperature throughout the separation procedures. LDL was isolated from plasma in the density interval I 025-1 050 kg// by sequential preparative ultracentrifugation [24,25] in a 50 3 Ti Bcckman fixed angle rotor (Beckman L8-80 ultracentrifuge; Palo Alto, CA) for 48 h. The total protein content of the LDL preparation was determined by the Lowry technique [26].…”

Section: Methodsmentioning

confidence: 99%

Autoantibodies against oxidized low density lipoproteins (oxLDL): characterization of antibody isotype, subclass, affinity and effect on the macrophage uptake of oxLDL

Wu¹,

Lefvert²

1995

Clinical and Experimental Immunology

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SUMMARY Circulating antibodies against oxLDL are present in several inflammatory and autoimmune conditions. Such antibodies are also present in patients with atherosclerosis, although the pathogenic significance of the antibodies is still not known. We have characterized the antibodies with regard to isotype, subclass, affinity and effect on macrophage uptake of oxLDL. Antibodies of IgG and IgM isotype were most common and found both in patients with atherosclerosis and in normal individuals. The subclass of IgG antibodies was mainly IgG2 and IgG3. Scatchard analyses of IgG and IgM antibodies showed that IgG antibodies were heterogeneous with regard to affinity, whereas only one population of high‐affinity antibodies was found in the IgM antibody population. The high‐affinity populations had an average equilibrium constant (KO) of 8.84 × 109 M−1 for IgG antibodies and of 1.65 × 109 M−1 for IgM antibodies. Incubation of 125I‐oxLDL with purified IgG and IgM from sera with high amounts of antibodies enhanced the uptake of 125I‐oxLDL in the monocyte‐like U937 cell line. Antibody preparations from sera containing no anti‐oxLDL antibodies and from sera with antibodies against LDL had less effect on this uptake. The increased uptake was competitively decreased by adding unlabelled oxLDL. This study shows that antibodies against oxLDL are mainly IgG2. IgG3 and IgM. Both IgG and IgM antibodies have a high affinity for the antigen and increase the uptake of oxLDL in a monocyte‐like ceil line.

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“…Serum lipoproteins were determined by preparative ultracentrifugation, precipitation and quantitative lipid analysis as described (Carlson, 1973). Cholesterol and triacylglycerols were determined by a modified colorimetric method and apolipoprotein B (apoB) by turbidity immunoassay (Rochel, Basel, Switzerland).…”

Section: Biochemical Analysismentioning

confidence: 99%

Simple advice on lifestyle habits and long-term changes in biomarkers of inflammation and vascular adhesion in healthy middle-aged men

Sjögren

Cederholm

Heimbürger³

et al. 2010

Eur J Clin Nutr

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Background/Objectives: Lifestyle habits, vascular function and inflammation are components in the development of cardiovascular disease (CVD). We investigated whether simple advice on dietary and exercise habits given (at a single time point) to hypercholesterolemic men affects circulating biomarkers of inflammation and vascular adhesion. Subjects/Methods: In total, 157 men (age 46±5 years) with mild hypercholesterolemia were randomized to four intervention groups, diet (D, n ¼ 40), exercise (E, n ¼ 39), diet and exercise (DE, n ¼ 39) or controls (C, n ¼ 39) and serum concentrations of C-reactive protein (CRP), interleukin-6 (IL-6), soluble intercellular adhesion molecule 1 (sICAM-1), soluble vascular cell adhesion molecule 1 (sVCAM-1) and soluble E-selectin (sE-selectin) were quantified at baseline and after a 6-month intervention period. Results: The intervention applied in this study, that is, simple advice on lifestyle changes given at a single time point, had a modest effect on inflammatory biomarkers and soluble vascular adhesion molecules. The most apparent alterations were found for individuals in group DE, who responded with significant reductions in sICAM-1, À28 (À41 to À14 mg/l) and sE-selectin, À3.6 (À6.9 to À0.3 mg/l) after 6 months. None of the groups had altered their concentrations of sVCAM-1, CRP or IL-6 significantly after the intervention. In all individuals combined, we found changes in apolipoprotein B (apoB) to predict alterations in sICAM-1 (b ¼ 0.21) and sE-selectin (b ¼ 0.26), independently of changes in inflammation and other adhesion molecules. Conclusions: These observations indicate that even small efforts to improve diet and physical activity can influence biomarkers of vascular function in individuals at increased risk for CVD. ApoB was identified as an important determinant of this improvement, which adds further support to the notion of apoB as a critical target in cardiovascular prevention.

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Lipoprotein fractionation.

Cited by 445 publications

References 10 publications

Insulin and non-esterified fatty acid relations to alimentary lipaemia and plasma concentrations of postprandial triglyceride-rich lipoproteins in healthy middle-aged men

Insulin and non-esterified fatty acid relations to alimentary lipaemia and plasma concentrations of postprandial triglyceride-rich lipoproteins in healthy middle-aged men

Autoantibodies against oxidized low density lipoproteins (oxLDL): characterization of antibody isotype, subclass, affinity and effect on the macrophage uptake of oxLDL

Simple advice on lifestyle habits and long-term changes in biomarkers of inflammation and vascular adhesion in healthy middle-aged men

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