Liposomal Amphotericin B Fosters the Corticosteroids’ Anti-inflammatory Effect on Murine Allergic Bronchopulmonary Aspergillosis Model Airways

Matsuse, Hiroto; Obase, Yasushi; Fukahori, Susumu; Tsuchida, Tomoko; Kawano, Tetsuya; Kohno, Shigeru; Mukae, Hiroshi

doi:10.1007/s10753-019-01069-z

Cited by 6 publications

(6 citation statements)

References 28 publications

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“…Exposure to A. fumigatus induces the degranulation of mast cells and basophils via IgE antibodies. Along with them, eosinophils interact directly with A. fumigatus conidia, produce extracellular traps, and injure the bronchial epithelium [ 4 , 21 , 22 ]. In this study, although IL-4 levels were under the detection threshold and IL-13 levels were low in patients with ABPA, their IL-5 levels were significantly higher than those in patients with CPA and were the second-highest after those in patients with asthma.…”

Section: Discussionmentioning

confidence: 99%

Serum Cytokines Usefulness for Understanding the Pathology in Allergic Bronchopulmonary Aspergillosis and Chronic Pulmonary Aspergillosis

Ito

Takazono

Obase

et al. 2022

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Allergic bronchopulmonary aspergillosis (ABPA) and chronic pulmonary aspergillosis (CPA) are important fungal infections caused by Aspergillus species. An overlap of ABPA and CPA has been reported; therefore, it is critical to determine whether the main pathology is ABPA or CPA and whether antifungals are required. In this study, we investigated whether the serum cytokine profile is useful for understanding the pathology and for differentiating between these diseases. We compared the various serum cytokine levels among healthy subjects and patients diagnosed with asthma, ABPA, or CPA at Nagasaki University Hospital between January 2003 and December 2018. In total, 14 healthy subjects, 19 patients with asthma, 11 with ABPA, and 10 with CPA were enrolled. Interleukin (IL) -5 levels were significantly higher in patients with ABPA than in those with CPA, and IL-33 and tumor necrosis factor (TNF) levels were significantly higher in patients with CPA than in those with asthma (p < 0.05, Dunn’s multiple comparison test). The sensitivity and specificity of the IL-10/IL-5 ratio (cutoff index 2.47) for diagnosing CPA were 70% and 100%, respectively. The serum cytokine profile is useful in understanding the pathology of ABPA and CPA, and the IL-10/IL-5 ratio may be a novel supplemental biomarker for indicating the pathology of CPA.

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Section: Discussionmentioning

confidence: 99%

Serum Cytokines Usefulness for Understanding the Pathology in Allergic Bronchopulmonary Aspergillosis and Chronic Pulmonary Aspergillosis

Ito

Takazono

Obase

et al. 2022

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“…2F). In ABPA management, it is still problem that there are only two creditable medications, OCS and anti-fungal medicines [1][2][3][4][5]. In this study, we expected the effect of LTRA and macrolide on airway eosinophilic or neutrophilic in ammation with or without corticosteroid.…”

Section: Discussionmentioning

confidence: 99%

“…Allergic bronchopulmonary aspergillosis (ABPA) caused by sensitization of the Aspergillus antigen, accounts for 1-4% of the asthma patients and involves in the asthma severity [1][2][3]. Exposure to fungal spores or mycelial fragments results in the formation of IgE and IgG antibodies, and T cells also play important roles in ABPA progress [4,5]. The working group of the International Society for Human and Animal Mycology (ISHAM) had suggested the disease criteria with a preceding lung disease (Bronchial asthma or cystic brosis), and have both type I allergy (type I skin reaction or speci c IgE antibody against Aspergillus antigen and/or increase of serum total IgE value) and type allergy (image ndings indicate the airway in ammation or bronchial injury [6].…”

Section: Introductionmentioning

confidence: 99%

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The Additional Effects of Clarithromycin And Pranlukast On The Cytokine Suppression By Corticosteroids Using Murine Allergic Bronchopulmonary Aspergillosis Model

Obase

Fukahori

Yamaguchi

et al. 2021

Preprint

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Few medicines other than oral corticosteroids and anti-fungal medicines are currently known as reliable treatments for allergic bronchopulmonary aspergillosis (ABPA). The efficacies of macrolide or leukotriene receptor antagonist (LTRA) with or without corticosteroid on ABPA are unknown. Mice were sensitized to Dermatophagoides farinae (Df) allergen intranasally and infected with Aspergillus fumigatus (Af). After Af infection, corticosteroid (Dexamethasone; Dex) was administered for five days in DfAf/Dex group. The effects of macrolide (clarithromycin; CAM) or LTRA (pranlukast; PRN) with or without Dex were also evaluated. Pathologically, the combined treatment with Dex and CAM decreased the airway inflammation. The interleukin (IL)-5, IL-13 and macrophage inflammatory protein (MIP)-2 concentrations in homogenized lungs were significantly elevated in DfAf mice compared to control mice (p < 0.05, each). CAM significantly decreased the elevations of MIP-2 of DfAf mice (p < 0.05). The addition of CAM on Dex suppressed both of the MIP-2 and IL-5 elevation (p < 0.05, each, DfAf/Dex vs DfAf/Dex/CAM group), but the addition of PRN on Dex did not. It was suggested that combination of CAM and corticosteroid enhanced the suppressing effect of both eosinophilic and neutrophilic airway inflammations. This finding will give a new hope for the treatment of severe fungus-related asthma.

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“…The protocol involved in preparing liposomal antibiotics has previously been described [25]. Briefly, 50 mg of MFX combined with 7.7 mg of cholesterol and 110.6 mg of DSPC (molar ratio = 14:15:2) were dissolved in 5 ml of methylene chloride.…”

Section: Preparation Of Mfx and Dex Containing Liposomes (Lipo-mfx/dex)mentioning

confidence: 99%

Liposomal dexamethasone–moxifloxacin nanoparticle combinations with collagen/gelatin/alginate hydrogel for corneal infection treatment and wound healing

et al. 2020

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Infectious keratitis is still one of the major causes of visual impairment and blindness, often affecting developing countries. Eye-drop therapy to reduce disease progression is the first line of treatment for infectious keratitis. The current limitations in controlling ophthalmic infections include rapid precorneal drug loss and the inability to provide long-term extraocular drug delivery. The aim of the present study was to develop a novel ophthalmic formulation to treat corneal infection. The formulation was prepared by constructing moxifloxacin (MFX) and dexamethasone (DEX)-loaded nanostructured lipid carriers (Lipo-MFX/DEX) mixed with a collagen/gelatin/alginate (CGA) biodegradable material (CGA-Lipo-MFX/DEX) for prolonged ocular application. The characteristics of the prepared Lipo-MFX/DEX nanoparticles were as follows: average size, 132.1 ± 73.58 nm; zeta potential, −6.27 ± 4.95 mV; entrapment efficiency, 91.5 ± 3.5%; drug content, 18.1 ± 1.7%. Our results indicated that CGA-Lipo-MFX/DEX could release an effective working concentration in 60 min and sustain the drug release for at least 12 h. CGA-Lipo-MFX/DEX did not produce significant toxicities, but it increased cell numbers when co-cultured with ocular epithelial cells. An animal study also confirmed that CGA-Lipo-MFX/DEX could inhibit pathogen microorganism growth and improve corneal wound healing. Our results suggest that CGA-Lipo-MFX/DEX could be a useful anti-inflammatory formulation for ophthalmological disease treatment.

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Liposomal Amphotericin B Fosters the Corticosteroids’ Anti-inflammatory Effect on Murine Allergic Bronchopulmonary Aspergillosis Model Airways

Cited by 6 publications

References 28 publications

Serum Cytokines Usefulness for Understanding the Pathology in Allergic Bronchopulmonary Aspergillosis and Chronic Pulmonary Aspergillosis

Serum Cytokines Usefulness for Understanding the Pathology in Allergic Bronchopulmonary Aspergillosis and Chronic Pulmonary Aspergillosis

The Additional Effects of Clarithromycin And Pranlukast On The Cytokine Suppression By Corticosteroids Using Murine Allergic Bronchopulmonary Aspergillosis Model

Liposomal dexamethasone–moxifloxacin nanoparticle combinations with collagen/gelatin/alginate hydrogel for corneal infection treatment and wound healing

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