Liposomal, Nanoparticle, and Conjugated Formulations of Anticancer Agents

Zamboni, William C.

doi:10.1158/1078-0432.ccr-05-1895

Cited by 212 publications

(217 citation statements)

References 55 publications

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“…Steric stabilization with polyethylene glycol (PEG) coating prolongs liposomes' circulation time while having a more even tissular distribution. These factors become very important characteristics in anti-cancer therapies where prolonged circulation of the chemotherapeutic agents is desired 12 .…”

Section: Discussionmentioning

confidence: 99%

“…Intravenous delivery in rodent models of siRNA via retro-orbital or tail vein injections has been proven harmless even in high concentrations, such as 50 mg/kg 10 . In the published literature the composition of cationic liposomes differs, based on the lipids formulation and their equimolar ratio 11,12 . There is a wide array of potential applications using cationic liposomes for gene delivery in vivo, whether targeting down-regulation/over-expression of the proteins, delivery of vaccines or anti-tumor therapies…”

Section: Introductionmentioning

confidence: 99%

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Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy

Bardita

Predescu

2013

JoVE

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Previous studies showed that knockdown of ITSN-1s (KD ITSN ), an endocytic protein involved in regulating lung vascular permeability and endothelial cells (ECs) survival, induced apoptotic cell death, a major obstacle in developing a cell culture system with prolonged ITSN-1s inhibition Here, efficient and specific KD ITSN in mouse lungs has been obtained using a cholesterol and dimethyl dioctadecyl ammonium bromide combination. Intravenous delivery of siRNA ITSN /cationic liposome complexes transiently knocked down ITSN-1s protein and mRNA in mouse lungs at day 3, which recovered after additional 3 days. Taking advantage of the cationic liposomes as a repeatable safe carrier, the study extended for 24 days. Thus, retro-orbital treatment with freshly generated complexes was administered every 3rd day, inducing sustained KD ITSN throughout the study 4 . Mouse tissues collected at several time points post-siRNA ITSN were subjected to electron microscopy (EM) analyses to evaluate the effects of chronic KD ITSN , in lung endothelium. High-resolution EM imaging allowed us to evaluate the morphological changes caused by KDITSN in the lung vascular bed (i.e. disruption of the endothelial barrier, decreased number of caveolae and upregulation of alternative transport pathways), characteristics non-detectable by light microscopy. Overall these findings established an important role of ITSN-1s in the ECs function and lung homeostasis, while illustrating the effectiveness of siRNA-liposomes delivery in vivo.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy

Bardita

Predescu

2013

JoVE

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“…Liposomes carrying chemotherapeutic smallmolecule drugs have been approved since the mid-1990s. Liposomes (∼100 nm and larger) can give extended circulation times if they are stabilized (see Doxil ® in Table 2) but do not provide intracellular delivery of drug molecules (78). Thus, they are not effective against disease that is resistant to cell surface pumps.…”

Section: Emerging Nanoparticle Therapeuticsmentioning

confidence: 99%

Nanotechnology and Cancer

2004

JAMA

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“…It has been shown that liposomes can act as biocompatible, biodegradable, and nonimmunogenic drug/gene carriers, and the advantages of liposomal-encapsulated drugs/genes are a prolonged duration of exposure, selective delivery to the intended site of action, and improved therapeutic index. 10,11 Among the liposomes, the Lipofectamine™ 2000 transfection reagent is a proprietary formulation for transfection of nucleic acids (DNA and RNA) into cells, and has been widely used by many researchers. [12][13][14] Liposomes can be modified with different kinds of ligands to control their biological properties such as longevity, targeting ability, and intracellular penetration, in a desired fashion.…”

Section: Introductionmentioning

confidence: 99%

Mannosylated liposomes for targeted gene delivery

Kong¹,

Ge²,

Liu³

et al. 2012

IJN

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Background: Liposomes can be modified with different ligands to control their biological properties, such as longevity, targeting ability, and intracellular penetration, in a desired fashion. The aim of this study was to modify liposomes with a novel mannosylated polyethylene glycolphosphatidylethanolamine (M-PEG-PE) ligand to achieve active targeted gene delivery. Methods: Rat Kupffer cells were isolated and used as model cells for in vitro evaluation of cytotoxicity and transfection efficiency. The modified liposomes were intravenously injected into the rats, and Kupffer cells were isolated and analyzed by flow cytometry for in vivo gene delivery and expression. Results: The M-PEG-PE-modified liposome-enhanced green fluorescence protein plasmid (M-PEG-PE-Lipo-pEGFP) complexes had a particle size of 237 nm and a loading efficiency of 90%. The M-PEG-PE-Lipo-pEGFP complexes displayed remarkably higher transfection efficiency than unmodified Lipo-pEGFP, both in vitro (51%-30%) and in vivo (43%-27%). Conclusion: M-PEG-PE could function as an excellent active targeting ligand, and M-PEG-PEmodified liposomes could be promising active targeted drug delivery vectors.

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Liposomal, Nanoparticle, and Conjugated Formulations of Anticancer Agents

Cited by 212 publications

References 55 publications

Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy

Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy

Nanotechnology and Cancer

Mannosylated liposomes for targeted gene delivery

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