Liposomal nanotransporter for targeted binding based on nucleic acid anchor system

Nejdl, Lukáš; Rodrigo, Miguel Ángel Merlos; Kudr, Jiří; Ruttkay-Nedecký, Branislav; Konečná, Marie; Kopel, Pavel; Zítka, Ondřej; Hubálek, Jaromír; Kizek, René; Adam, Vojtěch

doi:10.1002/elps.201300197

Cited by 5 publications

(4 citation statements)

References 71 publications

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“…The liposomes were prepared according to the published method [31] with necessary modifications [32]. Briefly, cholesterol (100 mg), 1,2-dioleoyl-sn-glycero-3-phospho-rac-(1-glycerol) sodium salt (100 mg) and phosphatidylcholine (100 mg) were dissolved in chloroform (4.5 mL).…”

Section: Methodsmentioning

confidence: 99%

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Platinum nanoparticles induce damage to DNA and inhibit DNA replication

et al. 2017

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Sparsely tested group of platinum nanoparticles (PtNPs) may have a comparable effect as complex platinum compounds. The aim of this study was to observe the effect of PtNPs in in vitro amplification of DNA fragment of phage λ, on the bacterial cultures (Staphylococcus aureus), human foreskin fibroblasts and erythrocytes. In vitro synthesized PtNPs were characterized by dynamic light scattering (PtNPs size range 4.8–11.7 nm), zeta potential measurements (-15 mV at pH 7.4), X-ray fluorescence, UV/vis spectrophotometry and atomic absorption spectrometry. The PtNPs inhibited the DNA replication and affected the secondary structure of DNA at higher concentrations, which was confirmed by polymerase chain reaction, DNA sequencing and DNA denaturation experiments. Further, cisplatin (CisPt), as traditional chemotherapy agent, was used in all parallel experiments. Moreover, the encapsulation of PtNPs in liposomes (LipoPtNPs) caused an approximately 2.4x higher of DNA damage in comparison with CisPt, LipoCisPt and PtNPs. The encapsulation of PtNPs in liposomes also increased their antibacterial, cytostatic and cytotoxic effect, which was determined by the method of growth curves on S. aureus and HFF cells. In addition, both the bare and encapsulated PtNPs caused lower oxidative stress (determined by GSH/GSSG ratio) in the human erythrocytes compared to the bare and encapsulated CisPt. CisPt was used in all parallel experiments as traditional chemotherapy agent.

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Section: Methodsmentioning

confidence: 99%

“…The homogenized mixtures were then heated and shaken for 15 min at 60°C using a Thermomixer Comfort (Eppendorf, Germany). The samples were then washed several times with water employing Amicon 3k centrifugation filters (Merck Millipore, Merck KgaA, Darmstadt, Germany) with a final sample volume of 1 mL [32]. …”

Section: Methodsmentioning

confidence: 99%

Platinum nanoparticles induce damage to DNA and inhibit DNA replication

et al. 2017

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“…The properties of design and optimization can be found in the following papers [42,43]. The properties of design and optimization can be found in the following papers [42,43].…”

Section: Spe Design and Fabricationmentioning

confidence: 99%

Remote‐controlled robotic platform ORPHEUS as a new tool for detection of bacteria in the environment

et al. 2014

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Remote-controlled robotic systems are being used for analysis of various types of analytes in hostile environment including those called extraterrestrial. The aim of our study was to develop a remote-controlled robotic platform (ORPHEUS-HOPE) for bacterial detection. For the platform ORPHEUS-HOPE a 3D printed flow chip was designed and created with a culture chamber with volume 600 μL. The flow rate was optimized to 500 μL/min. The chip was tested primarily for detection of 1-naphthol by differential pulse voltammetry with detection limit (S/N = 3) as 20 nM. Further, the way how to capture bacteria was optimized. To capture bacterial cells (Staphylococcus aureus), maghemite nanoparticles (1 mg/mL) were prepared and modified with collagen, glucose, graphene, gold, hyaluronic acid, and graphene with gold or graphene with glucose (20 mg/mL). The most up to 50% of the bacteria were captured by graphene nanoparticles modified with glucose. The detection limit of the whole assay, which included capturing of bacteria and their detection under remote control operation, was estimated as 30 bacteria per μL.

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“…Some thiol-containing compounds can be easily removed from the Au surface, thus, they have found applications where temporary nanoscale coatings were required [ 18 ]. Utilization the gold nanoparticles (AuNPs) modified liposome as a nanotransporter device with attraction to thiol modified oligonucleotides has been published, too [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Fluorescence Characterization of Gold Modified Liposomes with Antisense N-myc DNA Bound to the Magnetisable Particles with Encapsulated Anticancer Drugs (Doxorubicin, Ellipticine and Etoposide)

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Nejdl

Kudr

et al. 2016

Sensors

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Liposome-based drug delivery systems hold great potential for cancer therapy. The aim of this study was to design a nanodevice for targeted anchoring of liposomes (with and without cholesterol) with encapsulated anticancer drugs and antisense N-myc gene oligonucleotide attached to its surface. To meet this main aim, liposomes with encapsulated doxorubicin, ellipticine and etoposide were prepared. They were further characterized by measuring their fluorescence intensity, whereas the encapsulation efficiency was estimated to be 16%. The hybridization process of individual oligonucleotides forming the nanoconstruct was investigated spectrophotometrically and electrochemically. The concentrations of ellipticine, doxorubicin and etoposide attached to the nanoconstruct in gold nanoparticle-modified liposomes were found to be 14, 5 and 2 µg·mL−1, respectively. The study succeeded in demonstrating that liposomes are suitable for the transport of anticancer drugs and the antisense oligonucleotide, which can block the expression of the N-myc gene.

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Liposomal nanotransporter for targeted binding based on nucleic acid anchor system

Cited by 5 publications

References 71 publications

Platinum nanoparticles induce damage to DNA and inhibit DNA replication

Platinum nanoparticles induce damage to DNA and inhibit DNA replication

Remote‐controlled robotic platform ORPHEUS as a new tool for detection of bacteria in the environment

Fluorescence Characterization of Gold Modified Liposomes with Antisense N-myc DNA Bound to the Magnetisable Particles with Encapsulated Anticancer Drugs (Doxorubicin, Ellipticine and Etoposide)

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