Liposome destruction by a collapsing cavitation microbubble: A numerical study

Bacterial cell wall material properties determine E. coli resistance to sonolysis

“…The giant DOPC vesicles were destroyed in less than 5 s at 20 kHz and amplitude of 10 μm [81] . The results of decay of spheroplasts are consistent with lipid vesicle studies [82] and suggest that unprotected cytoplasmic membrane found in spheroplasts can be easily destroyed by cavitation. This also explains why animal cells, which do not have extra protective layers to stabilise cytoplasmic membrane, are very sensitive to sonication [83] .…”

Hydrodynamic cavitation efficiently inactivates potato virus Y in water

“…Since none of the tested ROS (e.g., • OH, 1 O 2 , and H 2 O 2 ) seemed to be involved in the PVY inactivation, it is very likely that the loss of its infectivity relies solely on the extreme mechanical events following the violent bubble collapses. Considering the filamentous PVY structure, the mechanical effects involved in inactivation most likely include the high-pressure gradients at the transition of the shockwaves, which are emitted during bubble collapses [24] , [54] . Petkovšek et al [20] performed an experimental study on shockwaves emitted during cavitation cloud collapse on the same Venturi constriction as used in the present study.…”

Cavitation bubble interaction with compliant structures on a microscale: A contribution to the understanding of bacterial cell lysis by cavitation treatment

“…2 . The presented methodology is largely based on our previous work [14] , [15] , where a more detailed description of the numerical model can be found.

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“…Here a brief overview of model setup and preliminary results is given. More details can be found in Appendix B and our previous work [14] , [15] . All simulations are performed for an axisymmetric case, as the presently employed methodology in a full 3D approach is well beyond our computational feasibility.…”

“…Our previous work suggests that during the inertial microbubble collapse the nearby submerged particle can be exposed to shear loads with peaks of a few megapascals and highly variable compressive loads in the form of shock waves with the magnitude in the order of a few hundred megapascals and gradients up to 100 MPa/ m [14] . Additionally, when one considers highly compliant biological structures, like liposomes, a collapsing bubble causes the nearby structure to deform according to the locally induced velocity field, which can lead to local membrane poration or even destruction of the liposome as a whole [15] . Results also suggest that larger bubbles might carry a higher potential for causing stretching-induced liposome destruction, although this has yet to be confirmed experimentally.…”