Liposome Electrokinetic Chromatography: An in vitro Approach for Predicting Ecotoxicity

De-ling, Xian; Huang, Kelong; Liu, Suqin; Xiao, Jun‐An

doi:10.1365/s10337-008-0538-1

Cited by 9 publications

(5 citation statements)

References 38 publications

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“…The LEKC mode has typically been used for drug or protein separation and for interaction studies in first years. Usually unmodified lipid vesicles of both natural and artificial sources have been applied in the running buffer . Lately, there has been an increase in diverse types of lipid dispersions added to the running buffer, namely PEG‐stabilized aggregates , proteoliposomes , lipid‐based nanoparticles , and commercial lipid dispersions .…”

Section: Liposomes In Ekc Pf‐ekc and Ce‐famentioning

confidence: 99%

Capillary electromigration techniques for studying interactions between analytes and lipid dispersions

Wiedmer

Lokajová

2012

J of Separation Science

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CE has matured into a well-known and much used separation technique, with applications covering more or less all types of samples. EKC, which originally was developed for the separation of uncharged compounds, is still today under much development, with main focus on finding the perfect or ideal carriers (pseudo-stationary phase) for a broad range of analytes. In this review, the use of lipid dispersions as pseudostationary phases in EKC performed in capillaries and microchips, in addition to CE frontal analysis and partial filling EKC using lipid dispersions is discussed. Various types of lipid dispersions including liposomes, PEG-stabilized aggregates, proteoliposomes, lipid-based nanoparticles, and commercial lipid emulsions are described. The purpose of the review is to give the reader an overview of how EKC, CE frontal analysis, and partial filling EKC, have been applied to the study of interactions between analytes and lipid membranes.

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Section: Liposomes In Ekc Pf‐ekc and Ce‐famentioning

confidence: 99%

Capillary electromigration techniques for studying interactions between analytes and lipid dispersions

Wiedmer

Lokajová

2012

J of Separation Science

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“…Computation proved good significant correlations between biological and chromatographic parameters, with R 2 values varying between 0.80 and 0.87. LEKC can be used for the rapid assessment of ecotoxicity of aromatic molecules (Xian et al, 2008b). The influence of cholesterol content of liposome, the pH and the ionic strength on the strength of the liposome ferulic acid interaction was investigated in detail using LEKC technology.…”

Section: Liposome Electrokinetic Capillary Chromatographymentioning

confidence: 99%

Liposomes in chromatography

Cserháti

Szőgyi

2010

Biomedical Chromatography

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The newest achievements in the application of liposomes in different chromatographic technologies such as high-performance liquid chromatography and electrically driven separation methods was compiled and critically evaluated. The employment of chromatographic methodologies for the determination of molecular parameters of biological importance is also discussed in detail. The future trends of the use of liposomes is also shortly discussed.

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“…Increasing concentration of cholesterol in the liposomes decreased the interactions between ferulic acid and the membrane because of decreased membrane fluidity. The same group published another LEKC study in 2008 and this time the focus was on LEKC as a tool for predicting the ecotoxicity of 15 selected aromatic compounds 36. The retention factors in LEKC were correlated with ecotoxicity parameters and gave correlation coefficients ( R 2 ) between 0.82 and 0.87.…”

Section: Liposomes As Carriers In Ekcmentioning

confidence: 99%

Liposomes in capillary electromigration techniques

Wiedmer

Shimmo

2009

Electrophoresis

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The use of phospholipid vesicles (liposomes) in EKC and CEC, as well as the use of CE for studying liposomes and lipid-analyte aggregates, are briefly reviewed. The works are presented in the order of appearance. The review describes the most common liposome dispersions used for achieving separation of neutral and charged compounds in liposome EKC. The CEC part is divided into two parts, discriminating between liposomes immobilized on capillaries for hindering analytes from interacting with the fused-silica wall and liposomes immobilized on capillaries for achieving chromatographic phases for separation of compounds. Finally, the use of CE for studying liposomes and lipid-analyte aggregates as analytes is discussed in brief.

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Liposome Electrokinetic Chromatography: An in vitro Approach for Predicting Ecotoxicity

Cited by 9 publications

References 38 publications

Capillary electromigration techniques for studying interactions between analytes and lipid dispersions

Capillary electromigration techniques for studying interactions between analytes and lipid dispersions

Liposomes in chromatography

Liposomes in capillary electromigration techniques

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