Liposomes containing biosurfactants isolated from Lactobacillus gasseri exert antibiofilm activity against methicillin resistant Staphylococcus aureus strains

Giordani, Barbara; Costantini, Paolo Emidio; Fedi, Stefano; Cappelletti, Martina; Abruzzo, Angela; Parolin, Carola; Foschi, Claudio; Frisco, Giulia; Calonghi, Natalia; Cerchiara, Teresa; Bigucci, Federica; Luppi, Barbara; Vitali, Beatrice

doi:10.1016/j.ejpb.2019.04.011

Cited by 56 publications

(35 citation statements)

References 34 publications

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“…While low concentrations (18 μM) of C 3 (CA) 2 dispersed about 60% of P. aeruginosa biofilms, the C3S mixture performed even better, removing up to 70% at subMIC (36 μM). In accordance with these results, it was recently reported that a biosurfactant loaded in phosphatidylcholine liposomes showed higher activity against MRSA biofilm than when free [ 57 ].…”

Section: Resultssupporting

confidence: 83%

“…Tack-seung Kim et al [ 59 ] recently found that lauroyl arginine ethyl ester effectively removed biofilm from reverse osmosis membranes. In general, cationic surfactants are more efficient at inhibiting the development of biofilms than dispersing them [ 57 , 59 ]. The efficiency of the studied catanionic systems in eradicating MRSA biofilms is similar to that reported for quaternary ammonium surfactants [ 58 ].…”

Section: Resultsmentioning

confidence: 99%

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Biocompatible Catanionic Vesicles from Arginine-Based Surfactants: A New Strategy to Tune the Antimicrobial Activity and Cytotoxicity of Vesicular Systems

et al. 2020

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Their stability and low cost make catanionic vesicles suitable for application as drug delivery systems. In this work we prepared catanionic vesicles using biocompatible surfactants: two cationic arginine-based surfactants (the monocatenary Nα-lauroyl-arginine methyl ester—LAM and the gemini Nα,Nϖ-bis(Nα-lauroylarginine) α, ϖ-propylendiamide—C3(CA)2) and three anionic amphiphiles (the single chain sodium dodecanoate, sodium myristate, and the double chain 8-SH). The critical aggregation concentration, colloidal stability, size, and charge density of these systems were comprehensively studied for the first time. These catanionic vesicles, which form spontaneously after mixing two aqueous solutions of oppositely charged surfactants, exhibited a monodisperse population of medium-size aggregates and good stability. The antimicrobial and hemolytic activity of the vesicles can be modulated by changing the cationic/anionic surfactant ratio. Vesicles with a positive charge efficiently killed Gram-negative and Gram-positive bacteria as well as yeasts; the antibacterial activity declined with the decrease of the cationic charge density. The catanionic systems also effectively eradicated MRSA (Methicillin-resistant Staphylococcus Aureus) and Pseudomonas aeruginosa biofilms. Interestingly, the incorporation of cholesterol in the catanionic mixtures improved the stability of these colloidal systems and considerably reduced their cytotoxicity without affecting their antimicrobial activity. Additionally, these catanionic vesicles showed good DNA affinity. Their antimicrobial efficiency and low hemolytic activity render these catanionic vesicles very promising candidates for biomedical applications.

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Section: Resultssupporting

confidence: 83%

Section: Resultsmentioning

confidence: 99%

Biocompatible Catanionic Vesicles from Arginine-Based Surfactants: A New Strategy to Tune the Antimicrobial Activity and Cytotoxicity of Vesicular Systems

et al. 2020

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“…saprophyticus , Enterobacter aerogenes , and Klebsiella pneumonia Cell wall-bound biosurfactants extraction in phosphate-buffered saline (PBS) [ 66 ] L. casei B1 Biosurfactants In vitro human epithelial cell line (HEp-2) Anti-oxidative, anti-proliferative, and anti-adhesion activity against S. aureus Cell wall-bound biosurfactants extraction in phosphate-buffered saline [ 190 ] Lactobacillus spp. Biosurfactants In vitro Antagonistic effects against E. coli Chloroform and methanol extraction [ 191 ] L. gasseri Biosurfactants In vitro Antibiofilm ability against methicillin-resistant S. aureus (MRSA) Cell wall-bound biosurfactants extraction in phosphate-buffered saline [ 192 ] L. paracasei D3-5 strain LTA In vivo Anti-aging Cell disruption by mechanical method and LTA extraction by butanol method [ 103 ] L. plantarum LTA In vitro Antibiofilm activity against S . mutans Mechanical cell disruption method of extraction followed by MALDI-TOF/MS based structure confirmation [ 108 ] L. plantarum LTA In vitro Anti-adhesion and antimicrobial effects Mechanical disruption of cells, n-butanol extraction followed by confirmation of LTA by conventional silver staining after polyacrylamide gel electrophoresis [ 193 ] L. plantarum K8 …”

Section: The Forms Of Postbiotics and Paraprobioticsmentioning

confidence: 99%

Postbiotics-parabiotics: the new horizons in microbial biotherapy and functional foods

et al. 2020

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Probiotics have several health benefits by modulating gut microbiome; however, techno-functional limitations such as viability controls have hampered their full potential applications in the food and pharmaceutical sectors. Therefore, the focus is gradually shifting from viable probiotic bacteria towards non-viable paraprobiotics and/or probiotics derived biomolecules, so-called postbiotics. Paraprobiotics and postbiotics are the emerging concepts in the functional foods field because they impart an array of health-promoting properties. Although, these terms are not well defined, however, for time being these terms have been defined as here. The postbiotics are the complex mixture of metabolic products secreted by probiotics in cell-free supernatants such as enzymes, secreted proteins, short chain fatty acids, vitamins, secreted biosurfactants, amino acids, peptides, organic acids, etc. While, the paraprobiotics are the inactivated microbial cells of probiotics (intact or ruptured containing cell components such as peptidoglycans, teichoic acids, surface proteins, etc.) or crude cell extracts (i.e. with complex chemical composition)". However, in many instances postbiotics have been used for whole category of postbiotics and parabiotics. These elicit several advantages over probiotics like; (i) availability in their pure form, (ii) ease in production and storage, (iii) availability of production process for industrial-scale-up, (iv) specific mechanism of action, (v) better accessibility of Microbes Associated Molecular Pattern (MAMP) during recognition and interaction with Pattern Recognition Receptors (PRR) and (vi) more likely to trigger only the targeted responses by specific ligand-receptor interactions. The current review comprehensively summarizes and discussed various methodologies implied to extract, purify, and identification of paraprobiotic and postbiotic compounds and their potential health benefits.

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“…Among pharmaceutical applications, BS antibacterial, antiviral, antifungal and anticancer effects have been reported [21,24,25]. Recently, two studies have been focused on the formulation of BS, isolated from a vaginal L. gasseri strain, inside liposomes active against Candida and methicillin resistant Staphylococcus aureus strains [26,27].…”

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confidence: 99%

Biosurfactant from vaginal Lactobacillus crispatus BC1 as a promising agent to interfere with Candida adhesion

et al. 2020

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Background: Lactobacillus spp. dominating the vaginal microbiota of healthy women contribute to the prevention of urogenital and sexually transmitted infections. Their protective role in the vagina can be mediated by Lactobacillus cells themselves, metabolites or bacterial components, able to interfere with pathogen adhesion and infectivity. Vulvovaginal candidiasis (VVC) is a common genital infection, caused by the overgrowth of opportunistic Candida spp. including C. albicans, C. glabrata, C. krusei and C. tropicalis. Azole antifungal drugs are not always efficient in resolving VVC and preventing recurrent infections, thus alternative anti-Candida agents based on vaginal probiotics have gained more importance. The present work aims to chemically characterize the biosurfactant (BS) isolated from a vaginal Lactobacillus crispatus strain, L. crispatus BC1, and to investigate its safety and antiadhesive/antimicrobial activity against Candida spp., employing in vitro and in vivo assays. Results: BS isolated from vaginal L. crispatus BC1 was characterised as non-homogeneous lipopeptide molecules with a critical micellar concentration value of 2 mg/mL, and good emulsification and mucoadhesive properties. At 1.25 mg/mL, the BS was not cytotoxic and reduced Candida strains' ability to adhere to human cervical epithelial cells, mainly by exclusion mechanism. Moreover, intravaginal (i.va.) inoculation of BS in a murine experimental model was safe and did not perturb vaginal cytology, histology and cultivable vaginal microbiota. In the case of i.va. challenge of mice with C. albicans, BS was able to reduce leukocyte influx. Conclusions: These results indicate that BS from vaginal L. crispatus BC1 is able to interfere with Candida adhesion in vitro and in vivo, and suggest its potential as a preventive agent to reduce mucosal damage occasioned by Candida during VVC.

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Liposomes containing biosurfactants isolated from Lactobacillus gasseri exert antibiofilm activity against methicillin resistant Staphylococcus aureus strains

Cited by 56 publications

References 34 publications

Biocompatible Catanionic Vesicles from Arginine-Based Surfactants: A New Strategy to Tune the Antimicrobial Activity and Cytotoxicity of Vesicular Systems

Biocompatible Catanionic Vesicles from Arginine-Based Surfactants: A New Strategy to Tune the Antimicrobial Activity and Cytotoxicity of Vesicular Systems

Postbiotics-parabiotics: the new horizons in microbial biotherapy and functional foods

Biosurfactant from vaginal Lactobacillus crispatus BC1 as a promising agent to interfere with Candida adhesion

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