Lipoxygenase-allene oxide synthase pathway in octocoral thermal stress response

Metabolite profiling of symbiont and host during thermal stress and bleaching in a model cnidarian-dinoflagellate symbiosis

“…11,14,17-Eicosatrienoic acid (C20_3n-3,6,9c) 13,16-Docosadienoic acid (C22_2n-6,9c) Stearic acid (C18_0) Palmitic acid (C16_0) Concentration ( Capnella imbricata (Lõhelaid et al, 2015). The observed reductions of C20 PUFAs in host pools can therefore be considered indicative of prolonged oxidative stress and highlight the possibility that oxylipin-based signalling cascades may also operate in Aiptasia.…”

A Catalase-related Hemoprotein in Coral Is Specialized for Synthesis of Short-chain Aldehydes

“…These products and the gene expression of the cHPL-LOX were measureable in coral samples under baseline conditions and after wounding (16) and thermal-induced stress (a change in sea water temperature from 23°C to 28°C or 31°C) (53). In contrast to the increased gene expression of the cAOS-LOX fusion protein induced both by wounding and thermal stress, the gene expression of the cHPL-LOX was stable during these test conditions.…”

“…In contrast to the increased gene expression of the cAOS-LOX fusion protein induced both by wounding and thermal stress, the gene expression of the cHPL-LOX was stable during these test conditions. There are few possible interpretations of this apparent lack of enhancement of the cHPL-LOX activity; (i) in contrast to its plant counterpart, the cHPL may not be involved in a response to mechanical injury, and (ii) given that plant oxylipin synthesis is activated within seconds or a few minutes of wounding (54,55), a short-lived increase in the cHPL-LOX products may occur immediately after wounding, which has not been analyzed (16,53). Activation of the plant HPL pathway is associated with changes in the expression of other defense genes (47,49,56), and equivalent experiments to explore effects of the coral lyase products are required to elucidate the role of cHPL-LOX pathway.…”

Evaluation and validation of reference genes for qPCR analysis to study climate change-induced stresses in Sinularia cf. cruciata (Octocorallia: Alcyonidae)

“…HSP70 is one of the members of the heat shock protein family that functions as a molecular chaperone and is involved in protein biogenesis as well as stress responses. HSP70 gene expression has been used as an indicator of a coral's stress status (van Oppen and Gates, 2006), and its response to thermal stress (Haguenauer et al, 2013;Lõhelaid et al, 2014) and air exposure (Teixeira et al, 2013) have been investigated in octocorals. The response of this gene to low seawater pH has not been investigated so far, despite the potential resilience of octocorals to decreased oceanic pH (Gabay et al, 2014).…”

“…The apparent increase in oxidative stress at the cellular level (Lesser, 2006) leads to apoptosis and necrosis (Richier et al, 2006;Tchernov et al, 2011), and thus, severely affects coral health (Lough, 2008), ultimately causing partial or complete colony mortality and mass bleaching events (Brown, 1997;Hughes et al, 2003). To gain a deeper understanding of the transcriptional response of corals to different sources of abiotic stress and the resulting adverse effects and/or potential resilience, different tools for gene expression profiling have been utilized (Lõhelaid et al, 2014;Maor-Landaw et al, 2014;Pratlong et al, 2015). Among them, quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR), or simply qPCR, is a reliable, reproducible and highly sensitive tool for the quantification of selected mRNA transcript levels.…”