Liquid-Based Cytology: A 25-Year Bridge between the Pap Smear and Molecular Cytopathology

Zeppa, Pio

doi:10.1159/000369593

Cited by 17 publications

(14 citation statements)

References 17 publications

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“…Air drying and improper dispersions of cells during the processing of CS remain primary causes of inadequate cytological results . LBC, which does not require special technical training or skills to collect the material, is a well‐established method for the diagnosis of cervical carcinoma . Over the past few years, the LBC techniques ThinPrep and SurePath have been extensively implemented for the cytological diagnosis of non‐gynecological specimens, moreover, LBC has been widely used for respiratory tract specimens .…”

Section: Discussionmentioning

confidence: 99%

The role of endobronchial ultrasound‐guided transbronchial needle aspiration liquid‐based cytology in the diagnosis of mediastinal lymphadenopathy

Qin

Lei

et al. 2019

Diagnostic Cytopathology

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Background Endobronchial ultrasound‐guided transbronchial needle aspiration (EBUS‐TBNA) is a minimally invasive, reliable technique for sampling mediastinal lymph nodes (LNs). Liquid‐based cytology (LBC) is widely used for cervical cancer screening because it provides reliable and feasible results. The present study aimed to evaluate effectiveness of the combination of EBUS‐TBNA and LBC in the diagnosis of mediastinal lymphadenopathy. Methods A total of 602 LNs that were retrospectively analyzed were sampled in 442 patients who underwent EBUS‐TBNA between January 2014 and December 2016. The histopathological result of TBNA tissue or cell blocks was considered as the gold standard to evaluate diagnostic utility of LBC and conventional smears (CS) for the diagnosis of mediastinal lymphadenopathy. Results Of the 602 LNs, 265 were mediastinal LN metastases from lung cancer, four were lymphoma, and 333 were benign. The sensitivity of LBC and CS in the diagnosis of mediastinal LN metastases from lung cancer was 72.8% and 63%, respectively, and the specificity was 98.5% and 97%, respectively. The positive predictive values for LBC and CS were 97.5% and 94.4%, respectively, whereas the negative predictive values were 82.2% and 76.9%, respectively. The accuracy of LBC and CS was 88% and 83.7%, respectively. The diagnostic value of LBC was significantly higher than that of CS (P = .001). Conclusions The combination of EBUS‐TBNA and LBC is a highly reliable and feasible procedure that optimizes diagnostic utility for the diagnosis of lung cancer and mediastinal LN staging.

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Section: Discussionmentioning

confidence: 99%

The role of endobronchial ultrasound‐guided transbronchial needle aspiration liquid‐based cytology in the diagnosis of mediastinal lymphadenopathy

Qin

Lei

et al. 2019

Diagnostic Cytopathology

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“…LBC offers the advantage of easy transportation; optimal cell preservation due to immediate fixation; storage at room temperature; and no special technical training or skills required for collecting the material in the fixative solution, which can solve the problem of sample assessment in settings in which cytotechnologists or cytopathologists are not available for ROSE . Similar to CBs, cellularity assessment cannot be performed at the time of sample acquisition.…”

Section: Introductionmentioning

confidence: 99%

Preanalytic specimen triage: Smears, cell blocks, cytospin preparations, transport media, and cytobanking

Santos

Saieg²

2017

Cancer Cytopathology

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With increasing requests for the evaluation of prognostic and predictive molecular biomarkers, great attention must be paid to the preanalytical issues regarding sample quality and DNA/RNA yield from all different types of cytological preparations. The objectives of this review were: 1) to provide an update regarding the importance of specimen triage as well as specimen handling and collection; 2) to discuss the different cell preparations that can be used for molecular testing, their advantages and limitations; and 3) to highlight the strategies for biobanking cytology samples. Good-quality DNA/ RNA can be harvested from fresh cells in cell suspensions, formalin-fixed paraffin-embedded cell blocks, archival stained smears, archival unstained cytospin preparations, liquid-based cytology slides, FTA cards, and cryopreserved cells. In contrast to formalin-fixed paraffin-embedded tissue specimens (small biopsies and surgical resections), the multitude of types of sample preparations as well as the diversity in sample collection and processing procedures make cytology an ideal specimen for most genomic platforms, with less DNA and RNA degradation and a purer sample, usually with a higher concentration of tumor cells. The broad incorporation of cytological specimens into clinical practice. A should increase the number of samples potentially available for molecular tests and avoid repeat invasive procedures for tissue procurement, thereby increasing patient safety. In this context, it is of utmost importance that cytopathologists become familiar with the variables that can affect test results and embrace the goal of excellence in sample quality. Cancer Cytopathol 2017;125(6 suppl):455-64.

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“…[1] Since its introduction, majority of laboratories have started to apply LBC technique in exfoliative cells and also in non-gynecological aspiration like fine needle aspiration (FNA) samples. [2] The advantages of LBC include rapid fixation, even distribution of cells over a smaller slide area, and decreased obscuring background elements, such as blood, inflammation, and mucus.…”

Section: Introductionmentioning

confidence: 99%

Utility of manual liquid-based cytology and conventional smears in the evaluation of various fine-needle aspiration samples

Arul

2016

J Cytol

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Background:Liquid-based cytology (LBC) preparation is a way to improve and refine the fine-needle aspiration (FNA) samples. There are a few studies comparing LBC with conventional smear (CS).Aim:The present study was undertaken to evaluate the utility of manual LBC (MLBC) and CS preparations in various FNA samples.Materials and Methods:In this cross-sectional study, a total of 100 FNA samples from various anatomical sites were evaluated using MLBC and CS preparations. Cellularity, blood, informative background, monolayers, cell architecture, cytoplasmic, and nuclear preservation were compared with MLBC and CS preparations by Wilcoxon signed rank test. P < 0.05 is considered statistically significant.Results:MLBC preparations were superior to CS preparations in view of absence of blood and debris (P = 0.001), presence of monolayers (P < 0.001), and preservation of cytoplasmic (P = 0.001) and nuclear details (P = 0.001). However, no statistically significant differences were found between MLBC and CS preparations with regard to cellularity (P = 0.157), informative background (P = 0.083), and architecture (P = 0.739).Conclusion:MLBC preparations in FNAC are a safe, easy, and less time-consuming procedure, and it may have promising diagnostic value in the evaluation of FNA samples from various anatomical sites. However, the use of both MLBC and CS preparations is recommended to achieve optimal diagnostic yield.

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Liquid-Based Cytology: A 25-Year Bridge between the Pap Smear and Molecular Cytopathology

Cited by 17 publications

References 17 publications

The role of endobronchial ultrasound‐guided transbronchial needle aspiration liquid‐based cytology in the diagnosis of mediastinal lymphadenopathy

The role of endobronchial ultrasound‐guided transbronchial needle aspiration liquid‐based cytology in the diagnosis of mediastinal lymphadenopathy

Preanalytic specimen triage: Smears, cell blocks, cytospin preparations, transport media, and cytobanking

Utility of manual liquid-based cytology and conventional smears in the evaluation of various fine-needle aspiration samples

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