Liquid cation exchange—a basis for sensitive radiometric assays for aromatic amino acid decarboxylases

McCaman, Marilyn W.; McCaman, Richard E.; Lees, G.J.

doi:10.1016/0003-2697(72)90024-3

Cited by 115 publications

(30 citation statements)

References 19 publications

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“…The product I4C-DA was separated by the method of McCaman et al (1972) and the enzyme activity calculated as nmoles I4C-DA/mg tissue/hour. and cortex were dissected from the whole brain, and the remaining subcortical area was homogenized in 5 volumes of 0.32 M sucrose.…”

Section: Methodsmentioning

confidence: 99%

Neurochemical effects of cocaine following acute and repeated injection

Taylor

1977

J of Neuroscience Research

106

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Following repeated injection in the rat, cocaine decreased the concentration of serotonin in the septum-caudate and increased the metabolism of hypothalamic norepinephrine and also striatal dopamine to a lesser extent. Furthermore, cocaine significantly decreased the activity of the rate-limiting enzyme, tryptophan hydroxylase. In a comparative study d-amphetamine and methylphenidate were found to exert an effect opposite to cocaine in the activation of tryptophan hydroxylase. These findings indicate that cocaine may lower central serotonin function by decreasing its availability for neural transmission. This could account for the stimulation of locomotor activity observed after cocaine administration.

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Section: Methodsmentioning

confidence: 99%

Neurochemical effects of cocaine following acute and repeated injection

Taylor

1977

J of Neuroscience Research

106

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“…Also shown are DDC specific activities from each stage from which the RNA was isolated. The DDC activity profiles show the expected activity at pupariation and adult eclosion (22,26). A prior experiment utilizing separated probes showed that the 0.0-kb DDC gene-containing probe hybridized two RNA species migratng with lengths 2.1 and 3.2 kb and that the +11-kb probe hybridized a 0.8-kb RNA.…”

Section: Addc-i T-f I I Imentioning

confidence: 99%

“…This band has not been observed to puff during late larvalprepupal development (2). DDC gene expression is developmentally regulated, with major peaks of DDC activity at the time of pupariation and again at adult eclosion (15,22,26). The appearance of DDC activity at pupariation is dependent on the molting hormone ,B-ecdysone.…”

Section: Addc-i T-f I I Imentioning

confidence: 99%

“…It is localized primarily in the hypoderm. DDC activity is developmentally regulated, showing major peaks at the time of puparium formation and again at adult eclosion (22,26). The first major peak of enzyme activity parallels a rise in titer of the molting hormone ,B-ecdysone (16,20), and the available evidence suggests that 8i-ecdysone is required for the appearance of this enzymatic activity (20,25).…”

mentioning

confidence: 99%

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Isolation and characterization of the dopa decarboxylase gene of Drosophila melanogaster.

Hirsh¹,

Davidson²

1981

Mol. Cell. Biol.

100

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We have isolated chromosomal deoxyribonucleic acid clones containing the Drosophila dopa decarboxylase gene. We describe an isolation procedure which can be applied to other nonabundantly expressed Drosophila genes. The dopa decarboxylase gene lies within or very near polytene chromosome band 37C1-2. The gene is interrupted by at least one intron, and the primary mode of regulation is pretranslational. At least two additional sequences hybridized by in vivo ribonucleic acid-derived probes are found within a 35-kilobase region surrounding the gene. The developmental profile of ribonucleic acid transcribed from one of these regions differs from that of the dopa decarboxylase transcript.The dopa decarboxylase (DDC) gene of Drosophila melanogaster is of considerable interest for a study of gene regulation. It is a well-characterized genetic locus, and it displays a complex pattern of developmental expression which is hormonally regulated during at least part of the life cycle. We have initiated a study of the structure and expression of this gene using recombinant deoxyribonucleic acid (DNA) techniques.DDC is an essential enzyme in the pathway of sclerotinization, the process of cuticle tanning and hardening in Drosophila. It is localized primarily in the hypoderm. DDC activity is developmentally regulated, showing major peaks at the time of puparium formation and again at adult eclosion (22, 26). The first major peak of enzyme activity parallels a rise in titer of the molting hormone , B-ecdysone (16, 20), and the available evidence suggests that 8i-ecdysone is required for the appearance of this enzymatic activity (20,25). In contrast, the second major peak of activity, at adult eclosion, is not correlated with an elevated f8-ecdysone titer. This activity, which occurs some 90 h after pupariation, occurs when ,B-ecdysone levels are very low and have been low for some 40 h (16). Thus, the two major inductions of DDC activity appear to be induced by dissimilar mechanisms.The

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“…Perhaps this indicates that several of the genes involved in sclerotization are clustered in Drosophila. In addition, the developmental profile of Drosophila's dopa decarboxylase (147,148) is very similar to that of Calliphora, where ecdysone control has been demonstrated (116). It is tempting to speculate, therefore, that a hormonal control of the Drosophila enzymes occurs at the tran scriptional level, in which case the close linkage of several genes would not be coincidental.…”

Section: Dopa Decarbox Ylasementioning

confidence: 92%