Liquid chromatographic analysis of non-volatile strawberry compounds

Mangas, J. J.; Moreno, Josefa Barrero; Suárez, B.; Picinelli, Anna; Blanco, Domingo

doi:10.1007/bf02466581

Cited by 9 publications

(5 citation statements)

References 18 publications

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“…Citrate is a major organic acid in strawberries and in many other fleshy fruits (Ulrich, 1970). In addition to the two major organic acids, malate and citrate, we detected quinate, shikimate, succinate, and fumarate, in line with recent data (Fernandez-Trujillo et al, 1999;Mangas et al, 1998), and isocitrate in agreement with enzymatic data (Reyes et al, 1982). We did not detect tartrate or pyruvate.…”

Section: Osmolarity Soluble Sugarssupporting

confidence: 92%

Biochemical Changes during Fruit Development of Four Strawberry Cultivars

Moing¹,

Renaud²,

Gaudillère³

et al. 2001

jashs

112

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As genetic factors affect strawberry (Fragaria ×ananassa Duch.) fruit development and quality, changes in metabolite concentrations were studied during fruit development of four strawberry cultivars grown in the field: three commercial cultivars (Capitola, Elsanta and Dover) and a genotype from Centre Interrégional de Recherche et d'Expérimentation de la Fraise, France (`CF1116'). Major and minor metabolites changed with development. The two strawberry cultivars with the highest starch content at early stages, `Capitola' and `Elsanta', also had the highest fruit weight at harvest. There was no correlation between strawberry weight and osmolarity. At maturity, significant differences were observed among cultivars for most of the metabolites studied. `Capitola' and `Elsanta' responded similarly for most measured variables. `CF1116' was characterized by high juice osmolarity and high sucrose, inositol, glutamine, arginine and alanine concentrations, and low citrate and malate concentrations. `Dover' was characterized by a high galactose concentration and low asparagine and alanine concentrations. Organic acid differences among cultivars appeared early during development, while differences in soluble sugars appeared during maturation. The developmental pattern of each amino acid varied among cultivars. Timing of the biochemical differences observed among cultivars provides some information on their metabolic origin.

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Section: Osmolarity Soluble Sugarssupporting

confidence: 92%

Biochemical Changes during Fruit Development of Four Strawberry Cultivars

Moing¹,

Renaud²,

Gaudillère³

et al. 2001

jashs

112

View full text Add to dashboard Cite

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“…Many organic compounds present in foods and organic solvents strongly absorb in this zone of the spectrum. Therefore, the measurement of the change of refractive index (RI) is the preferred detection system in a wide range of food samples. , The main feature of this detector is its universality so derivatization steps are not required. However, RI detectors have several drawbacks: (i) low sensitivity and selectivity; (ii) their response is greatly influenced by environment changes such as pressure, temperature, and dissolved air content; and (iii) changes in the mobile-phase concentration lead to a modification in the signal, and hence, they are not suitable for analyses based on gradient elution.…”

mentioning

confidence: 99%

Simultaneous Determination of Carbohydrates, Carboxylic Acids, Alcohols, and Metals in Foods by High-Performance Liquid Chromatography Inductively Coupled Plasma Atomic Emission Spectrometry

et al. 2006

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The applicability of the HPLC-ICP-AES coupling for the simultaneous determination of carbohydrates, carboxylic acids, alcohols, and metals in a single chromatographic run has been demonstrated in the present work. Five saccharides, glucose, fructose, sucrose, sorbitol, and lactose; five carboxylic acids, citric, tartaric, malic, lactic, and acetic; and three alcohols, glycerol, ethanol, and methanol, have been determined. A H+ cation exchange column has been used to separate these compounds. The chromatograms have been obtained by monitoring the carbon emission signal at 193.09 nm. The results obtained by HPLC-ICP-AES have been compared against those found with conventional detection systems (i.e., refractive index, UV, and photodyode array detectors). The HPLC-ICP-AES method has shown the following features: (i) organic compounds and metals can be simultaneously determined; (ii) the detection method is universal; (iii) for nonvolatile organic compounds, a complete calibration line can be obtained from a single injection; and (iv) it provides absolute limits of detection similar to or lower than those found with conventional detection systems (i.e., on the order of several tens of nanograms of organic compound). The methodology has been validated through the analysis of food samples such as juices, isotonic beverages, wines, and a certified nonfat milk powder sample.

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“…The universal refractive index detector has been used extensively for the determination of major sugars present in food and beverage samples. Olive plants, strawberries, apple juices, wines, legume seeds, and citrus juices are a few of the substances recently assayed for carbohydrates using this mode of detection. − The practical utility of refractive index detection, however, is generally limited to major sugar determinations because of poor sensitivity. When the vulnerability to minor mobile-phase and environmental changes is coupled to the poor sensitivity of refractive index systems, this mode of detection is often “the choice of last resort”…”

mentioning

confidence: 99%

An Inductively Coupled Plasma Carbon Emission Detector for Aqueous Carbohydrate Separations by Liquid Chromatography

2000

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An inductively coupled plasma atomic emission spectrometer is used to detect carbon-containing compounds following separation by high-performance liquid chromatography. A calcium form ligand exchange column with distilled and deionized water as the mobile phase is used to separate carbohydrates. The eluting species are detected by monitoring the carbon atomic emission line at 193.09 nm. The mass detection limits using a photomultiplier tube for sucrose and glucose are 50 ng, while that for fructose is 60 ng. The carbon emission detector should provide the same detection limit for any compound with a similar mass percent of carbon, whether or not the compound exhibits appreciable absorption characteristics. While the carbon emission detector will universally detect any organic compound, it will discriminate against species with high molar absorptivity that may be present at low concentration. Such species may act as interferences in chromatograms generated with conventional UV-visible absorption detectors. To demonstrate the utility of the carbon emission detector, three sugars (glucose, fructose, sucrose) are determined in apple, crangrape, and orange juice.

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Liquid chromatographic analysis of non-volatile strawberry compounds

Cited by 9 publications

References 18 publications

Biochemical Changes during Fruit Development of Four Strawberry Cultivars

Biochemical Changes during Fruit Development of Four Strawberry Cultivars

Simultaneous Determination of Carbohydrates, Carboxylic Acids, Alcohols, and Metals in Foods by High-Performance Liquid Chromatography Inductively Coupled Plasma Atomic Emission Spectrometry

An Inductively Coupled Plasma Carbon Emission Detector for Aqueous Carbohydrate Separations by Liquid Chromatography

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