Liquid chromatographic analysis of pharmaceutical syrups using pre-columns and salt-adsorption on Amberlite XAD-2

Mohammed, Hussain Y.; Cantwell, Frederick F.

doi:10.1021/ac50025a033

Cited by 37 publications

(14 citation statements)

References 29 publications

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“…The literature survey revealed the availability of few methods for the assay of MDH in pharmaceutical formulations. Quantification of MDH has been achieved by high-performance liquid chromatography (HPLC) [4][5][6], liquid chromatography [7], spectrofluorimetry [8], differential fluorimetry, and differential UV-spectrophotometry [9]. Some visible spectrophotometric methods are also reported for the assay of on pharmaceuticals [10][11][12][13][14][15][16][17][18][19][20][21][22].…”

Section: Introductionmentioning

confidence: 99%

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Development and Validation of Stability-Indicating RP-UPLC Method for the Determination of Methdilazine in Bulk Drug and in Pharmaceutical Dosage Form

Raghu

Basavaiah

Xavier

et al. 2012

ISRN Chromatography

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A simple, precise, and accurate, and stability-indicating isocratic Ultraperformance Liquid Chromatography (UPLC) method was developed for the determination of methdilazine hydrochloride (MDH) in bulk drug and in its tablets. The use of UPLC, with a rapid 5-minute-reversed-phase isocratic separation on a 1.7 µm reversed-phase packing material to provide rapid "high throughput" support for MDH, is demonstrated. The method was developed using Waters Acquity BEH C18 column (100 mm × 2.1 mm, 1.7 µm) with mobile phase consisting of a mixture of potassium dihydrogenorthophosphate and 1-pentane sulphonic acid buffer of pH 4.0 and acetonitrile (60 : 40 v/v). The eluted compound was detected at 254 nm with a UV detector. The standard curve of mean peak area versus concentration showed an excellent linearity over a concentration range 0.5-80 µg mL −1 MDH with regression coefficient (r) value of 0.9999. The limit of detection (S/N = 3) was 0.2 µg mL −1 and the limit of quantification (S/N = 10) was 0.5 µg mL −1 . Forced degradation of the bulk sample was conducted in accordance with the ICH guidelines. Acidic, basic, hydrolytic, oxidative, thermal, and photolytic degradations were used to assess the stability indicating power of the method. The drug was found to be stable in acidic, basic, thermal, hydrolytic, and photolytic stress conditions and showed slight degradation in oxidative stress condition.

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Section: Introductionmentioning

confidence: 99%

“…Though a number of liquid chromatographic methods have earlier been proposed for MDH [4][5][6][7], no attempt has been made to apply this new type of LC (UPLC) for pharmaceuticals despite its many-fold advantages.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of Stability-Indicating RP-UPLC Method for the Determination of Methdilazine in Bulk Drug and in Pharmaceutical Dosage Form

Raghu

Basavaiah

Xavier

et al. 2012

ISRN Chromatography

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“…21 There are many methods for the simultaneous determination of paracetamol and caffeine, including spectrophotometric, [22][23][24][25] electroanalytical, 26,27 HPLC [28][29][30] and GC techniques. 31 HPLC and GC methods require expensive instrumentation and are relatively highly time-consuming.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Paracetamol and Caffeine by Flow Injection–Solid Phase Spectrometry Using C18 Silica Gel as a Sensing Support

2002

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Experimental ReagentsAll solutions were prepared from analytical reagent-grade chemicals using deionized water.Paracetamol ( Department of Physical and Analytical Chemistry, Faculty of Experimental Sciences, University of Jaén, Paraje Las Lagunillas, E-23071 Jaén, SpainA continuous and simple UV-photometric flow-through biparameter-sensing device has been developed for the simultaneous determination of paracetamol and caffeine at 275 nm. The sensor is based on temporary sequentiation in the arrival of the analytes to the sensing zone by on-line separation using C18 bonded phase beads (the same as that used in the sensing zone) placed into a minicolumn just before the flow cell. The sample containing these compounds is injected into the carrier solution; paracetamol is determined first because it passes through the minicolumn, while caffeine is strongly retained in it. Then, caffeine is conveniently eluted from the precolumn and develops its transitory signal. Using 200 µl of a sample and deionized water as a carrier, the analytical signal showed a very good linearity in the ranges of 10 -160 µg ml -1 and 3.5 -50 µg ml -1 with detection limits of 0.75 and 0.56 µg ml -1 for paracetamol and caffeine, respectively. If deionized water with the pH adjusted at 12 was used as a carrier solution, these parameters were 25 -400 and 4 -55 µg ml -1 with 2.0 and 0.50 µg ml -1 as the detection limits, respectively. The biparameter optosensor was satisfactorily applied to the simultaneous determination of these two analytes in pharmaceuticals.

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“…They are routinely used to concentrate trace components from both biological and environmental samples (1, 2). They have been used as packings for moderate efficiency liquid chromatography columns (3,4) and, in the form of microparticles, as reverse-phase packings for high-efficiency liquid chromatography columns (5-7). These polymers are essentially nonionic and tend to strongly adsorb hydrophobic, nonionic sample molecules from aqueous solution.…”

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Sorption of magnesium–picolinate complexes on a macroporous styrene–divinylbenzene copolymer containing immobilized 8-hydroxyquinoline and on the copolymer itself

Persaud¹,

Cantwell²

1992

Can. J. Chem.

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GOCOOL PERSAUD and FREDERICK F. CANTWELL. Can. J. Chem. 70, 926 (1992).The sorbent XAD-OXINE was prepared by covalently binding 8-hydroxyquinoline onto the surface of the macroporous copolymer Amberlite XAD-2 to give about 20% surface coverage. Sorption, onto both XAD-2 and XAD-OXINE, of the magnesium-containing species that are present in solutions of magnesium ion and picolinic acid was studied by the column equilibration/atomic absorption technique at solution pH of 6.00 and 7.50 and ionic strength of 0.25. By using solutions containing various amounts of added magnesium and picolinic acid, in which the ratio of the two metalligand complex species, MgP'IMgP,, varied greatly (P-is the ligand picolinate), it was shown that neither the species Mg2' nor the species MgP+ sorb to an appreciable extent onto the parent polymer XAD-2, but the neutral s ecies MgP, 6' +sorbs strongly and exhibits a linear isotherm. Then, by measuring the sorption isotherm of the species Mg-on XAD-OXINE in the absence of picolinic acid, by measuring the overall sorption of magnesium-containing species on XAD-OXINE in the presence of picolinic acid, and by assuming that the sorption of the species MgPz onto XAD-OXINE was about the same as it was on XAD-2, it was possible to show that the species MgP+ sorbs strongly onto XAD-OXINE, and exhibits linear sorption isotherms. Thus, XAD-OXINE sorbs all three species Mgz+, MgP+, and MgP,.Key words: Amberlite XAD-2, immobilized oxine, magnesium ion. Chem. 70, 926 (1992).On a prepark I'adsorbant XAD-OXINE en liant d'une fagon covalente de la 8-hydroxyquinoleine sur la surface du copolyrnere rnacroporeux Arnberlite XAD-2 de manibre a couvrir environ 20% de la surface. Macroporous copolymers of styrene and divinylbenzene (DVB) find many uses in analytical chemistry. They are routinely used to concentrate trace components from both biological and environmental samples (1, 2). They have been used as packings for moderate efficiency liquid chromatography columns (3, 4) and, in the form of microparticles, as reverse-phase packings for high-efficiency liquid chromatography columns (5-7). These polymers are essentially nonionic and tend to strongly adsorb hydrophobic, nonionic sample molecules from aqueous solution. However, they also sorb ionic sample species (8). They have been used as HPLC packings for "dynamic ion exchange" chromatography of lanthanide ions (9). In this latter type of application a large surfactant ion is reversibly sorbed onto the surface of all of the packing in the column and lanthanide sample ions are chromatographed on this reversibly modified packing.Macroporous styrene-DVB copolymers have also been derivatized by covalently attaching various groups to the polymer surface. Low-capacity cation and anion exchangers have been prepared by attaching sulfonate or quaternary 'Author to whom correspondence may be addressed. ammonium groups. They have been used as liquid chromatographic column packings for separating both organic and inorganic ions (10,11). A physico-chemical model has be...

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Liquid chromatographic analysis of pharmaceutical syrups using pre-columns and salt-adsorption on Amberlite XAD-2

Cited by 37 publications

References 29 publications

Development and Validation of Stability-Indicating RP-UPLC Method for the Determination of Methdilazine in Bulk Drug and in Pharmaceutical Dosage Form

Development and Validation of Stability-Indicating RP-UPLC Method for the Determination of Methdilazine in Bulk Drug and in Pharmaceutical Dosage Form

Simultaneous Determination of Paracetamol and Caffeine by Flow Injection–Solid Phase Spectrometry Using C18 Silica Gel as a Sensing Support

Sorption of magnesium–picolinate complexes on a macroporous styrene–divinylbenzene copolymer containing immobilized 8-hydroxyquinoline and on the copolymer itself

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