Liquid chromatographic and tandem mass spectrometric assay for evaluation of in vivo inhibition of rat brain monoamine oxidases (MAO) A and B following a single dose of MAO inhibitors: application of biomarkers in drug discovery

Lang, Wensheng; Masucci, John A.; Caldwell, Gary W.; Hageman, William; Hall, J. E.; Jones, Walter W.; Rafferty, Bryan M.

doi:10.1016/j.ab.2004.07.001

Cited by 17 publications

(7 citation statements)

References 43 publications

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“…Finally, it must be highlighted that one of the advantage of the use of UHPLC is the decreasing of the analysis time in relation to previous published methods. Thus, the total run time is 8 min, but when conventional LC is used (Lang et al, 2004;Bourcier et al, 2006), analysis time is higher than 25 min.…”

Section: Determination Of Neurotransmitters In Rat Brainmentioning

confidence: 99%

Development and validation of an ultra-high performance liquid chromatography–tandem mass-spectrometry (UHPLC–MS/MS) method for the simultaneous determination of neurotransmitters in rat brain samples

González¹,

Fernández²,

Vidal³

et al. 2011

Journal of Neuroscience Methods

148

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Section: Determination Of Neurotransmitters In Rat Brainmentioning

confidence: 99%

Development and validation of an ultra-high performance liquid chromatography–tandem mass-spectrometry (UHPLC–MS/MS) method for the simultaneous determination of neurotransmitters in rat brain samples

González¹,

Fernández²,

Vidal³

et al. 2011

Journal of Neuroscience Methods

148

View full text Add to dashboard Cite

“…A variety of methods have been developed to measure serotonin including spectrophotometry [21,22], LCfluorometry [23,24], enzyme immunoassay, and LCelectrochemical detection [25,26]. LC-mass spectrometric methods is however the method of choice as tandem LC-MS methods provide increased selectivity while reducing the need for dependence on chromatographic separation [27][28][29][30][31][32][33][34][35]. While these methods report good results for the analytes under investigation, the methods do not facilitate multi-target analysis which was the aim of this study.…”

Section: Introductionmentioning

confidence: 99%

Development of an LC-MS/MS method for the analysis of serotonin and related compounds in urine and the identification of a potential biomarker for attention deficit hyperactivity/hyperkinetic disorder

et al. 2011

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Serotonin is a major neurotransmitter and affects various functions both in the brain and in the rest of the body. It has been demonstrated that altered serotinergic function is implicated in various psychiatric disorders including depression and schizophrenia. Serotonin has also been implicated along with dopamine in attention deficithyperkinetic disorder (AD-HKD). This study provides a versatile validated method for the analysis of serotonin, hydroxyindole acetic acid and dopamine in urine using LC-MS/MS. This method was then used to quantify these analytes in a test group of 17 children diagnosed with severe AD-HKD. This group was compared to a matched control group to investigate the possibility that one of these compounds may be a potential biomarker for this condition. The developed method provided good linear calibration curves for the multiplex assay of analytes in urine (0.05-3.27 nmol/L; R 2 ≥0.9977). Acceptable inter-day repeatability was achieved for all analytes with RSD values (n=9) ranging from 1.1% to 9.3% over a concentration range of 0.11-3.27 μmol/L in urine. Excellent limits of detection (LOD) and limits of quantitation (LOQ) were achieved with LODs of 8.8-18.2 nmol/L and the LOQs of 29.4-55.7 nmol/L for analytes in urine. Recoveries were in the ranges of 98-104%, 100-106% and 91-107% for serotonin, 5-HIAA and dopamine, respectively. An appropriate sample clean-up procedure for urine was developed to ensure efficient recovery and reproducibility on analysis. Evaluation of matrix effects was also carried out and the influence of ion suppression on analytical results reported. Confirmatory analysis was carried out on a linear trap quadrupole-Orbitrap mass spectrometer to obtain high mass accuracy data of the target analytes in the clinical samples.

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“…Analyses using capillary electrophoresis (CE) with UV or mass spectrometry (MS) detection have also been reported 11–15. LC and CE coupled with tandem mass spectrometry (MS/MS)16–24 have been used to increase the specificity of analyses. Recently, mixtures of few catecholamines in brain tissue17, 18 and in urine19–21 were analyzed by LC/MS/MS with good sensitivity.…”

mentioning

confidence: 99%

Detection of 28 neurotransmitters and related compounds in biological fluids by liquid chromatography/tandem mass spectrometry

Bourcier

Benoist

Clerc

et al. 2006

Rapid Comm Mass Spectrometry

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This work presents two liquid chromatography/tandem mass spectrometry (LC/MS/MS) acquisition modes: multiple reaction monitoring (MRM) and neutral loss scan (NL), for the analysis of 28 compounds in a mixture. This mixture includes 21 compounds related to the metabolism of three amino acids: tyrosine, tryptophan and glutamic acid, two pterins and five deuterated compounds used as internal standards. The identification of compounds is achieved using the retention times (RT) and the characteristic fragmentations of ionized compounds. The acquisition modes used for the detection of characteristic ions turned out to be complementary: the identification of expected compounds only is feasible by MRM while expected and unexpected compounds are detected by NL. In the first part of this work, the fragmentations characterizing each molecule of interest are described. These fragmentations are used in the second part for the detection by MRM and NL of selected compounds in mixture with and without biological fluids. Any preliminary extraction precedes the analysis of compounds in biological fluids.

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Liquid chromatographic and tandem mass spectrometric assay for evaluation of in vivo inhibition of rat brain monoamine oxidases (MAO) A and B following a single dose of MAO inhibitors: application of biomarkers in drug discovery

Cited by 17 publications

References 43 publications

Development and validation of an ultra-high performance liquid chromatography–tandem mass-spectrometry (UHPLC–MS/MS) method for the simultaneous determination of neurotransmitters in rat brain samples

Development and validation of an ultra-high performance liquid chromatography–tandem mass-spectrometry (UHPLC–MS/MS) method for the simultaneous determination of neurotransmitters in rat brain samples

Development of an LC-MS/MS method for the analysis of serotonin and related compounds in urine and the identification of a potential biomarker for attention deficit hyperactivity/hyperkinetic disorder

Detection of 28 neurotransmitters and related compounds in biological fluids by liquid chromatography/tandem mass spectrometry

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