Liquid Chromatographic Assay for the Analysis of Kanamycin sulphate nanoparticles in Rat after intramuscular administration: Application to a Pharmacokinetic Study

Mustafa, Sanaul; Devi, V. Kusum

doi:10.7324/japs.2016.60809

Cited by 6 publications

(4 citation statements)

References 13 publications

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“…Accuracy was with the deviation between the nominal concentration and calculated concentration for CUR well below the limit of ± 15%. The results obtained for the determination of precision and accuracy were reproducible and robust 20 .…”

Section: Results and Discussion: Specificity And Selectivitymentioning

confidence: 69%

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2022

IJPSR

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Curcumin is used as a potent anti-inflammatory agent in the treatment of rheumatoid arthritis and its clinical pharmacokinetics requires a sensitive method for estimation of its plasma concentration. Hence the aim of the study was to develop a rapid, selective, and sensitive HPLC method coupled with UV detection for the determination of Curcumin in rat plasma. The process of elution was carried out on Phenomenex Luna C18 (250*4.6 mm id, 5 μm particle size, 130 Å) column using acetonitrile and 1 % formic acid mixture in the ratio of 70:30 % v/v as mobile phase delivered with a flow rate of 1.0 mL/min. Detection was carried out using a UV detector at 420 nm. The method was found to be linear in the range of 100 to 3000 ng/ml with R2 close to one (0.9969). The limit of detection (LOD) and limit of quantification (LOQ) of CUR was found to be 2.82 ng/mL and 8.5 ng/mL, respectively. The method was validated for accuracy, precision, linearity, LOD, LOQ, and robustness. Hence the method developed can be used in routine analysis of curcumin in rat plasma which can be useful to determine the pharmacokinetics of curcumin.

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Section: Results and Discussion: Specificity And Selectivitymentioning

confidence: 69%

“…Followed collection, it was subjected to centrifugation to extract the plasma. The collected plasma was stored in a deep freezer at -4 °C until further analysis 19,20 .…”

Section: Separation Of Plasmamentioning

confidence: 99%

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2022

IJPSR

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“…• Mustafa S and Devi K reported a method to detect Kenamycin using a mobile phase containing 0.1M disodium tetraborate (pH 9.0) and water (25:75) with Phenomenex C18 column under isocratic condition using 205 nm wavelength [19].…”

Section: Liquid Chromatography (Lc)mentioning

confidence: 99%

Analytical Methods for the Determination of Aminoglycosides Antibiotics by Chromatrographic Technique

Sofiqul

Murugan

Kumari

2020

Int J Pharm Pharm Sci

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Aminoglycosides antibiotics are considered to be the antimicrobial agents used frequently in the treatment of human diseases caused by a bacterial infection. Most of the aminoglycosides antibiotics are highly polar in nature and they are lacking the UV absorbing chromophore in the molecules. The present articles accentuate the analytical method associated with the analysis of aminoglycosides molecules. Various chromatographic techniques like liquid chromatography, gas chromatography; mass spectrometry were used for the detection of aminoglycosides antibiotics. However, due to its limitation in the ultraviolet-visible spectrophotometry (UV/Vis) technique, different types of detection techniques like corona-charged aerosol detector (CAD), electrochemical detector (ECD) were used as a most powerful and versatile technique for the demonstration of these molecules in the analytical field. Analytical methods help to ensure the quality of the drug products. This review paper is devoted to providing an overview of the key performance technique used for the application and detection of these aminoglycosides molecules.

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“…Five standard solutions were prepared by serial dilution of SER stock solution (1000 g/ml) in the range of 50, 100, 110, 120, 130, 140 and 150 μg/ml in order to determine the limit of detection (LOD) and limit of quantification (LOQ). LOD and LOQ were calculated according to LOD = 3.3 σ/S and LOQ = 10 σ /S, where σ is the standard deviation of the response and S is the slope of the calibration curve 13 .…”

Section: Limit Of Detection and Limit Of Quantificationmentioning

confidence: 99%

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2019

IJPSR

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A novel method of estimation and validation of Sertaconazole nitrate (SER) by Reverse Phase-High Performance Liquid Chromatography coupled with Ultra-violet detection was developed which had high potential in determining drug concentration with more precision and high accuracy. The process of elution was conducted using Phenomenex C 18 (250 mm × 4.6 mm i.d., 5.0 μm) using 0.01M monobasic sodium phosphate and acetonitrile in a ratio of 28:72% v/v as mobile phase at 4.5 pH and a flow rate of 1.0 mL/min. Detection was carried out using a UV detector at 260 nm. This precise method was linear between a range of 10 to 500 μg/ml with R 2 close to one (0.999). The limit of detection (LOD) and limit of quantification (LOQ) of SER was found to be 0.1 μg/ml and 0.15 μg/ml respectively. The method was validated for accuracy, precision, linearity, LOD, LOQ, and robustness. Validation studies demonstrated that this HPLC method is simple, specific, rapid, reliable and reproducible. The 3-level 2-factor facecentred central composite design was employed using Design Expert Software ver. 7.0.0 to examine the effect of independent chromatographic factors like pH of the mobile phase and the ratio between 0.01M monobasic sodium phosphate and acetonitrile on the dependent factors like peak area, theoretical plates and tailing factor. The ANOVA studies proved that the model employed for this study was significant. This method can be used as a more convenient and efficient option for the analysis of SER to establish the quality of the drug substance during routine analysis with consistent and reproducible results.

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Liquid Chromatographic Assay for the Analysis of Kanamycin sulphate nanoparticles in Rat after intramuscular administration: Application to a Pharmacokinetic Study

Cited by 6 publications

References 13 publications

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Analytical Methods for the Determination of Aminoglycosides Antibiotics by Chromatrographic Technique

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