Liquid chromatographic determination of bisphenols based on intramolecular excimer-forming fluorescence derivatization

Determination of Catecholamines and Indoleamines in Human Urine Based on Intramolecular Excimer-forming Derivatization and Fluorescence Detection

“…In our previous method, 13 Tyr and Tyra in urine could be determined without any pretreatments, owing to the selectivity of intramolecular excimer-forming fluorescence derivatization. [14][15][16] In this study, however, deproteinization with perchloric acid and solid-phase extraction were applied as a pretreatment of urine samples to obtain simpler chromatograms and to prevent any damage of the column. As shown in Table 2, when we applied the pretreatment procedure described in 487 ANALYTICAL SCIENCES APRIL 2007, VOL.…”

“…The resulting dipyrene-labeled derivatives provided intramolecular excimer fluorescence at the wavelength region of 440 -520 nm, which was shifted markedly to higher emission wavelengths, as compared to the wavelengths of the monopyrene-labeled monofunctional compounds (360 -420 nm). This chemistry allowed up to perform the selective analysis of tyrosine and tyramine, even in complex samples containing monoaminergic and monophenolic compounds, as is the cases with polyamine 14,15 and bisphenol 16 analyses.…”

Highly Selective and Simple Method for Determination of Polythiols Based on Liquid Chromatography with Postcolumn Excimer Fluorescence Derivatization

“…[11][12][13][14][15][16][17][18][19][20][21][22] Pyrene-containing precolumn derivatization reagents such as 4-(1-pyrene)butanoic acid N-hydroxysuccinimide ester, [11][12][13]18 4-(1-pyrene)butanoyl chloride, 14,17,19,21 4-(1-pyrene) butanoic acid hydrazide, 15,16,20 N-(1-pyrene)iodoacetamide, 22 and N-(1-pyrenemethyl)iodoacetamide 22 were reacted with corresponding reactive functional groups of target molecules. The resulting polypyrene-labeled derivatives exhibited intramolecular excimer fluorescence at wavelength of 440 -520 nm, which was a significant shift towards longer wavelengths when compared to those of derivatization reagents and monopyrene-labeled derivatives (360 -420 nm).…”

“…E-mail: masayama@fukuoka-u.ac.jp selective determination of polyfunctional compounds was possible even in complex samples containing monofunctional compounds. [11][12][13][14][15][20][21][22] However, precolumn derivatization procedures, such as the addition of reagents, heating, and neutralization before injection, are required, even though the excimer fluorescence derivatization method is selective and sensitive. Therefore, a simple and convenient procedure is desired for analysis.…”

Liquid Chromatographic Determination of Ethambutol in Serum Samples Based on Intramolecular Excimer-forming Fluorescence Derivatization

“…All of these polyamino compounds were converted to the corresponding polypyrene-labeled derivatives by a reaction with 4-(1-pyrene)butyric acid Nhydroxysuccinimide ester (PSE), and the derivatives generated an intramolecular excimer fluorescence. Recently, we have developed the intramolecular excimer-forming fluorescence determination methods for bisphenols 21 and polyamines 22 by using 4-(1-pyrene)butanoyl chloride (PBC), whose reactivity to the amino group is higher than that of PSE. In this study, we applied the derivatization technique with PBC to the determination of EMB.…”