Liquid Chromatographic Determination of Ethambutol in Serum Samples Based on Intramolecular Excimer-forming Fluorescence Derivatization

Nakano, Yoritaka; Nohta, Hitoshi; Yoshida, Hideyuki; Todoroki, Kenichiro; Saita, Tetsuya; Fujito, Hiroshi; Mori, Masato; Yamaguchi, Masatoshi

doi:10.2116/analsci.20.489

Cited by 15 publications

(12 citation statements)

References 23 publications

(19 reference statements)

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“…19,20 In the methods, the primary and secondary amino moieties in histamine were labeled with a pyrene reagent, 4-(1-pyrene)butyric acid N-hydroxysuccinimide ester (PSE), and the excited dipyrenelabeled derivative afforded an intramolecular excimer to fluoresce in a wavelength region (440 -540 nm) longer than that for the usual pyrene derivatives (360 -420 nm). By using these characteristics, not only histamine, but also other polyamines, [21][22][23][24] were determined with high selectivity and sensitivity, even though the sample was contaminated by monoamino compounds.…”

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confidence: 99%

Simultaneous Determination of Histamine and Histidine by Liquid Chromatography Following Intramolecular Excimer-forming Fluorescence Derivatization with Pyrene-labeling Reagent

et al. 2004

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confidence: 99%

Simultaneous Determination of Histamine and Histidine by Liquid Chromatography Following Intramolecular Excimer-forming Fluorescence Derivatization with Pyrene-labeling Reagent

et al. 2004

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“…In our previous method, 13 Tyr and Tyra in urine could be determined without any pretreatments, owing to the selectivity of intramolecular excimer-forming fluorescence derivatization. [14][15][16] In this study, however, deproteinization with perchloric acid and solid-phase extraction were applied as a pretreatment of urine samples to obtain simpler chromatograms and to prevent any damage of the column. As shown in Table 2, when we applied the pretreatment procedure described in 487 ANALYTICAL SCIENCES APRIL 2007, VOL.…”

Section: Urine Assaymentioning

confidence: 99%

“…The resulting dipyrene-labeled derivatives provided intramolecular excimer fluorescence at the wavelength region of 440 -520 nm, which was shifted markedly to higher emission wavelengths, as compared to the wavelengths of the monopyrene-labeled monofunctional compounds (360 -420 nm). This chemistry allowed up to perform the selective analysis of tyrosine and tyramine, even in complex samples containing monoaminergic and monophenolic compounds, as is the cases with polyamine 14,15 and bisphenol 16 analyses.…”

Section: Introductionmentioning

confidence: 99%

Determination of Catecholamines and Indoleamines in Human Urine Based on Intramolecular Excimer-forming Derivatization and Fluorescence Detection

et al. 2007

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Experimental Reagents, solutions, and materialAll of the chemicals and solvents were of highest purity available, and were used as received. Distilled water, which was further purified with a MilliQ gradient system (Millipore, Milford, MA, USA), was used to prepare all aqueous solutions. PBC was obtained from Toronto Research Chemicals (North York, Ontario, Canada), and was used without further purification.Stock solutions (1.0 mM) of bioactive amines were prepared in water and stored at 4˚C. These solutions were stable for at least one week, and were diluted further with acetonitrile to the required concentrations before use. A 5 mM solution of PBC A liquid chromatographic (LC) determination of catecholamines and indoleamines is described. This is based on intramolecular excimer-forming fluorescence derivatization with 4-(1-pyrene)butanoyl chloride, followed by reversedphase LC. The analytes, containing an amino moiety and phenolic hydroxyl moieties in a molecule, were converted to the corresponding polypyrene-labeled derivatives by one-step derivatization. They afforded intramolecular excimer fluorescence, which can clearly be discriminated from the normal fluorescence emitted from reagent blanks. The detection limits (S/N = 3) for catecholamines and indoleamines were femto-mole levels per 20-μL injection. Furthermore, this method was applied to a urine assay.

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“…Most of these techniques focus on analytical separations under chromatographic (including thin layer chromatography) [4][5][6][7][8] or electrophoretic conditions [9][10][11][12]. In some cases, ethambutol undergoes to chemical reactions such as complexation with copper ions [10], formation of ionic-pairs [13,14] and derivatisation processes with different reagents [15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Flow Injection Analysis of Ethambutol in Antituberculosis Drugs Using a Graphite‐Paraffin Electrode as Amperometric Detector

et al. 2011

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A graphite-paraffin composite electrode has been used for the determination of ethambutol, an antituberculosis drug, in pharmaceutical samples by anodic oxidation. Voltammetric studies were used to characterise the electrochemical behaviour of the ethambutol in different pH solutions (3.0, 8.0 and 12.0). Quantification was carried out using amperometric detection. The detector was assembled in a flow injection apparatus and operated at + 1.1 V (vs. Ag/AgCl (NaCl,sat) ). The conditions for the best electrodes response were determined by studying the influence of sample volume and flow rate. The linear response for the method was extended up to a 1.5 mmol L À1 ethambutol solution with a detection limit of 0.1 mmol L À1. The repeatability for successive injections of 1.0 mmol L À1 ethambutol solution was 0.9 % (n = 26), demonstrating a good performance of the developed amperometric sensor. The analytical frequency was calculated as approximately 47 determinations h À1. Two different commercial samples were successfully analysed and the results were in good agreement with those obtained by using spectrophotometry. The advantages of the proposed amperometric method over the reference method include simplicity, low consumption of reagents and increased analytical frequency.

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Liquid Chromatographic Determination of Ethambutol in Serum Samples Based on Intramolecular Excimer-forming Fluorescence Derivatization

Cited by 15 publications

References 23 publications

Simultaneous Determination of Histamine and Histidine by Liquid Chromatography Following Intramolecular Excimer-forming Fluorescence Derivatization with Pyrene-labeling Reagent

Simultaneous Determination of Histamine and Histidine by Liquid Chromatography Following Intramolecular Excimer-forming Fluorescence Derivatization with Pyrene-labeling Reagent

Determination of Catecholamines and Indoleamines in Human Urine Based on Intramolecular Excimer-forming Derivatization and Fluorescence Detection

Flow Injection Analysis of Ethambutol in Antituberculosis Drugs Using a Graphite‐Paraffin Electrode as Amperometric Detector

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