Simultaneous Determination of Histamine and Histidine by Liquid Chromatography Following Intramolecular Excimer-forming Fluorescence Derivatization with Pyrene-labeling Reagent

Liquid Chromatographic Determination of Ethambutol in Serum Samples Based on Intramolecular Excimer-forming Fluorescence Derivatization

“…In previous studies for the determinations of polyamino compounds, PSE [15][16][17][18][19][20] and PBC 22 were used as pyrene-labeling reagents for amino groups. EMB was reacted only with highly reactive PBC, probably due to hindrance of the bulky alkyl groups with the derivatization reaction.…”

“…In our previous research, we have already developed highly selective fluorometric determination methods for polyamines, 15 basic amino acids, 16 triethylenetetramine, 17 and histamine, [18][19][20] based on intramolecular excimer-forming fluorescence derivatization. All of these polyamino compounds were converted to the corresponding polypyrene-labeled derivatives by a reaction with 4-(1-pyrene)butyric acid Nhydroxysuccinimide ester (PSE), and the derivatives generated an intramolecular excimer fluorescence.…”

An easy-to-use excimer fluorescence derivatization reagent, 2-chloro-4-methoxy-6-(4-(pyren-4-yl)butoxy)-1,3,5-triazine, for use in the highly sensitive and selective liquid chromatography analysis of histamine in Japanese soy sauces

“…The use of dansyl chloride further results in an excess of unreacted reagents, which are detected as large peaks in the chromatograms and interfere with Him quantification. Yoshitake et al described a fluorometric LC method to determine Him concentrations in human urine, rat brain microdialysate and rat plasma and tissue, based on an intramolecular excimer-forming fluorescence derivatization with 4-(1-pyrene) butyric acid N-hydroxysuccinimide ester (PSE) [16][17][18][19]. The resulting polypyrene-labeled derivatives of Him provided intramolecular excimer fluorescence at a wavelength of 440-520 nm.…”

Highly Selective and Simple Method for Determination of Polythiols Based on Liquid Chromatography with Postcolumn Excimer Fluorescence Derivatization

“…[11][12][13][14][15][16][17][18][19][20][21][22] Pyrene-containing precolumn derivatization reagents such as 4-(1-pyrene)butanoic acid N-hydroxysuccinimide ester, [11][12][13]18 4-(1-pyrene)butanoyl chloride, 14,17,19,21 4-(1-pyrene) butanoic acid hydrazide, 15,16,20 N-(1-pyrene)iodoacetamide, 22 and N-(1-pyrenemethyl)iodoacetamide 22 were reacted with corresponding reactive functional groups of target molecules. The resulting polypyrene-labeled derivatives exhibited intramolecular excimer fluorescence at wavelength of 440 -520 nm, which was a significant shift towards longer wavelengths when compared to those of derivatization reagents and monopyrene-labeled derivatives (360 -420 nm).…”