Liquid Chromatographic Determination of the Constituents in Shao-yao-tang and Related Chinese Herbal Preparations

Sheu, Shuenn‐Jyi; Li, Kwei-Lan

doi:10.1002/(sici)1521-4168(19981001)21:10<569::aid-jhrc569>3.3.co;2-i

Cited by 6 publications

(6 citation statements)

References 8 publications

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“…Applications of chromatographic techniques to medicinal plants and Chinese traditional medicines are outlined [49]. Methods using gradient elution HPLC with reversed phase columns had been applied for the analysis of multiple constituents present in medicinal plants and herbal preparations [50][51][52]. Gradient elution HPLC with ultraviolet detection, using a C18 reversed phase column had been used to profile components present in C. rhizoma, Radix aristolochiae, ginseng, R. glycyrrhizae (liquorice), S. radix, R. codonopsis pilosula and S. miltiorrhiza [34,36,38,45,46].…”

Section: Analysis Of Botanicals and Herbal Preparation By Liquid Chromentioning

confidence: 99%

Extraction methods and chemical standardization of botanicals and herbal preparations

Ong

2004

Journal of Chromatography B

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Section: Analysis Of Botanicals and Herbal Preparation By Liquid Chromentioning

confidence: 99%

Extraction methods and chemical standardization of botanicals and herbal preparations

Ong

2004

Journal of Chromatography B

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“…4 Mainly, reverse phaseliquid chromatography (RP-HPLC) used for the estimation of various chemical compounds existing in single drug or mixture of drugs and its herbal formulations. 5 Adhatoda vasica Nees (Acanthaceae), also known as Vasaka in Ayurveda, is a perennial shrub prevalent in the hot regions of Southeast Asia. 6 The plant is extensively used in Indian system of medicine (ISM) for the medication of respiratory system related ailments such as cough, asthma, allergeninduced bronchial obstruction and it has been used as an herbal remedy for tuberculosis.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Quantification of Lupeol, Stigmasterol and β- Sitosterol in Extracts of Adhatoda vasica Nees Leaves and its Marketed Formulations by a Validated RP-HPLC Method

Nandhini¹,

Ilango²

2020

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Background: Adhatoda vasica Nees (Acanthaceae) is a well-known medicinal plant used for the treatment of respiratory disorders such as asthma and bronchitis. Objective: To develop a simple and precise RP-HPLC method for the simultaneous assessment of lupeol, stigmasterol and β-sitosterol of various extracts of Adhatoda vasica Nees. Materials and Methods: The compounds were separated on RP-Phenomenex C 18 (250mm×4.6mm; 5µ) column with a mobile phase comprising of 0.1%v/v formic acid in water and methanol (28:82%v/v) splashed at a flow of 0.8mL/min with PDA detector at 208nm. Results: The retention time of lupeol, stigmasterol and β-sitosterol was found to be 16.89, 18.26 and 20.72 minutes respectively. The amount of lupeol was abundant in hexane extract (0.952%w/w) and formulation III (23.72ng/g) whereas, stigmasterol (0.285%w/w) and β-sitosterol (8.649%w/w) was highly abundant in chloroform extract and formulation I stigmasterol (2.57ng/g) and β-sitosterol (0.98ng/g). The optimized method was validated for different parameters and all the validated constraints were within the limits as per ICH guidelines. The proposed method was linear over the concentration range of 12.5-200µg/mL with correlation coefficients greater than 0.997. The LOD and LOQ values of lupeol, stigmasterol and β-sitosterol were found to be 0.66, 5.64 and 12.8µg/mL and 2.01, 17.10 and 36.62µg/mL respectively. Conclusion: To conclude, the developed method for the simultaneous estimation of lupeol, stigmasterol and β-sitosterol was simple, precise, accurate and thus reliable for the quality control investigations of crude drugs and its herbal formulations.

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“…The advances in chromatographic separation techniques made it possible to quantify the chemical constituents in a mixture with comparatively little cleanup [3][4]. Particularly, methods using high-performance liquid chromatography (HPLC) with reversed phase columns are most commonly applied to the analysis of multiple constituents present in medicinal plants and herbal preparations [5].…”

Section: Introductionmentioning

confidence: 99%

A Novel Rp-HPLC Method for Simultaneous Estimation of Berberine, Quercetin, and Piperine in an Ayurvedic Formulation

Sahani

Jain

2019

Int J App Pharm

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Objective: The objective of this study was to develop and validate a novel, simple, rapid, precise and accurate reversed-phase high performance liquid chromatographic (RP-HPLC) method for simultaneous quantitative estimation of berberine, quercetin, and piperine in Ayurvedic formulation.Methods: The chromatographic separation was achieved using a stationary phase C18 shim-pack (150 mm x 4.6 mm, 5µ) column and mobile phase consisted of acetonitrile: 0.04 M potassium dihydrogen phosphate buffer (pH 3.0 adjusted using orthophosphoric acid) in a ratio of 65:35 v/v, with a flow rate of 1 ml/min and UV detection at 255 nm.Results: The retention time of berberine, quercetin, and piperine were found to be 2.7, 3.0 and 6.3 min respectively. Linearity for berberine, quercetin, and piperine were found in the range of 12-28 µg/ml. All calibration curve showed good linear correlation coefficients (r2˃ 0.999) within the tested ranges. Mean percent recoveries for berberine, quercetin, and piperine were found to be within the acceptance limits (98-120%). The percent relative standard deviation (% RSD) for precision was found to be less than 2% which indicates method is precise.Conclusion: The developed method is novel, simple, precise, accurate and can be used for quantitative analysis and quality control of the raw material as well as other commercial formulations containing these three markers.

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Liquid Chromatographic Determination of the Constituents in Shao-yao-tang and Related Chinese Herbal Preparations

Cited by 6 publications

References 8 publications

Extraction methods and chemical standardization of botanicals and herbal preparations

Extraction methods and chemical standardization of botanicals and herbal preparations

Simultaneous Quantification of Lupeol, Stigmasterol and β- Sitosterol in Extracts of Adhatoda vasica Nees Leaves and its Marketed Formulations by a Validated RP-HPLC Method

A Novel Rp-HPLC Method for Simultaneous Estimation of Berberine, Quercetin, and Piperine in an Ayurvedic Formulation

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