No abstract

A high-performance liquid chromatographic method for the separation of twenty one constituents of Shao-yao-tang including six flavonoids, six alkaloids, three anthraquinones, three carboxylic acids, one monoterpene, one saponin, and one aldehyde was developed. Detection at 275 nm with a linear gradient elution system consisting of tetrabutylammonium bromide (TBA) and acetic acid solution was found to be suitable for determination of nineteen marker substances in an unpretreated Shao-yao-tang extract within 75 min. This method can also be used to analyze 11 -14 components in a number of Chinese herbal preparations such as San-huang-hsieh-hsin-tang, Chingchieh-lien-chiao-tang, San-tsung-kuei-chien-tang, Chai-hu-ching-kantang, and Ching-yin-li-ke-tang.

A high‐performance liquid chromatographic method for the separation of twenty one constituents of Shao‐yao‐tang including six flavonoids, six alkaloids, three anthraquinones, three carboxylic acids, one monoterpene, one saponin, and one aldehyde was developed. Detection at 275 nm with a linear gradient elution system consisting of tetrabutylammonium bromide (TBA) and acetic acid solution was found to be suitable for determination of nineteen marker substances in an unpretreated Shao‐yao‐tang extract within 75 min. This method can also be used to analyze 11–14 components in a number of Chinese herbal preparations such as San‐huang‐hsieh‐hsin‐tang, Ching‐chieh‐lien‐chiao‐tang, San‐tsung‐kuei‐chien‐tang, Chai‐hu‐ching‐kan‐tang, and Ching‐yin‐li‐ke‐tang.