Determination of flavonoids and alkaloids in the scute-coptis herb couple by capillary electrophoresis

Li, Kwei-Lan; Sheu, Shuenn‐Jyi

doi:10.1016/0003-2670(95)00190-b

Cited by 66 publications

(27 citation statements)

References 7 publications

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“…Several researchers have utilized CE to analyze for various metabolites found in plants including members of the flavonoid family [23]. Sources of the flavonoids include commonly used herbs [24][25][26], common foods [27][28][29], and fruits [30]. In this report, we describe the development of a MEKC-based assay used to profile isoflavonoids related to the biosynthetic pathway of medicarpin.…”

Section: Introductionmentioning

confidence: 99%

Profiling isoflavonoids found in legume root extracts using capillary electrophoresis

et al. 2002

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Legumes such as alfalfa (Medicago sativa L.), barrel medic (Medicago truncatula), white sweet clover (Melilotus alba) and fenugreek (Trigonella graecum), normally accumulate (-)-medicarpin and its malonated glucose conjugate as natural inhibitors of fungal pathogens. These plants also accumulate the biosynthetic precursor formononetin as well as the malonated glycoside. We were interested in developing a robust high-throughput method to quantitate the levels of these two isoflavonoids, both free and conjugated, in legume root extracts, for use in screening for mutant plants accumulating altered levels of these compounds. Capillary electrophoresis was examined as an alternative to current high-performance liquid chromatography (HPLC) methods to generate isoflavonoid profiles. The developed assay used micellar electrokinetic capillary chromatography (MEKC) to provide the required selectivity in complex root extracts. The addition of 1,2-hexanediol to the sodium dodecyl sulfate (SDS) electrolyte provided improved resolution of adjacent isoflavonoids. We examined the role of several factors including sample preparation, buffer composition, buffer pH, and organic component in the injected sample. The use of capillaries with longer path lengths were also examined to increase sensitivity. A comparison of results obtained using MEKC and HPLC showed good correlation in the relative amounts of the isoflavonoids studied.

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Section: Introductionmentioning

confidence: 99%

Profiling isoflavonoids found in legume root extracts using capillary electrophoresis

et al. 2002

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“…Flavonoids are abundant in RS and six flavones are identified to be its major ingredients, namely baicalein (B), baicalin (BG), wogonin (W), wogonoside (WG), oroxylin A (OA) and oroxylin A-7-glucuronide (OAG) (Li et al, 2009;Li & Sheu, 1995) (Figure 1). In our previous studies on the pharmacokinetic characterization of bioactive flavones in RS, it was found that B, W and OA could be absorbed directly and underwent extensive Phase II metabolism mainly mediated by UDP-glucuronosultransferases (UGTs) (Li et al, 2012a,b;Zhang et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Influence of mefenamic acid on the intestinal absorption and metabolism of three bioactive flavones inRadix Scutellariaeand potential pharmacological impact

Fong²,

Mei

et al. 2013

Pharmaceutical Biology

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, whereas their percentage of metabolism was decreased from 72.75 AE 2.44%, 73.27 AE 3.25% and 89.84 AE 2.99% to 21.11 AE 0.69%, 17.90 AE 5.55% and 45.44 AE 3.38%. PGE 2 level was much lower in the co-administration group (49.04 AE 2.03 pg/ml) than in the MEF group (73.13 AE 3.03 pg/ml) or RS group (494.37 AE 11.75 pg/ml) 4 h post MEF dosing, suggesting a synergic effect. Discussion and conclusion: Co-administration of MEF and RS could induce potential alterations in their pharmacokinetic profiles and anti-inflammatory effects.

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“…Nevertheless, most reports have focused on the detection and determination of alkaloids in the plants and have paid little attention to the potential of CE in the construction of fingerprints for distinguishing different species and their identification [9][10][11][12][13][14]. At present, the identification of various species is performed by morphology and TLC.…”

Section: Introductionmentioning

confidence: 99%

Characterization of 10 species ofMahonia by capillary electrophoresis

Liu

et al. 2000

Chromatographia

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SummaryA simple, rapid and effective capillary electrophoresis method was developed for the characterization of 10 different species of Mahonia. A fingerprint of the extract of each species was constructed using a mixed buffer of borate and phosphate, containing methanol, pH 8.0. The effective electrophoretic mobility and % normalized area of each peak in electrophoregrams were evaluated to characterize various species. Three alkaloids: berberine, palmatine and jatrorrhizine were found in all 10 species. The CE technique appears to provide a powerful tool for the identification and quality control of plant drugs.

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Determination of flavonoids and alkaloids in the scute-coptis herb couple by capillary electrophoresis

Cited by 66 publications

References 7 publications

Profiling isoflavonoids found in legume root extracts using capillary electrophoresis

Profiling isoflavonoids found in legume root extracts using capillary electrophoresis

Influence of mefenamic acid on the intestinal absorption and metabolism of three bioactive flavones inRadix Scutellariaeand potential pharmacological impact

Characterization of 10 species ofMahonia by capillary electrophoresis

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