Characterization of 10 species ofMahonia by capillary electrophoresis

Li, Yang; Ji, Xiuhong; Liu, Huwei; Yan, Yi; Li, Jiashi

doi:10.1007/bf02490617

Cited by 21 publications

(7 citation statements)

References 8 publications

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“…It is a stimulus for the central nervous system, causes the sense organ to be more sensitive, stimulates the visceral organs and urogenital tract. Similarly, berberine in medicinal plants or CPM products are determined using ultrasonic extraction with CZE [9][10][11][12] and CE-ESI-MS [13]. However, the margin between useful and dangerous doses is very narrow as it was reported to be fatal to man at doses of 30-90 mg [1,5].…”

Section: Introductionmentioning

confidence: 99%

Determination of berberine and strychnine in medicinal plants and herbal preparations by pressurized liquid extraction with capillary zone electrophoresis

Ong

Apandi

2001

Electrophoresis

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A simple and rapid method for the determination of berberine and strychnine in medicinal plants and herbal preparations for regulatory purposes using a home-made pressurized liquid extraction (PLE) system with capillary zone electrophoresis (CZE) using ultraviolet detection at 254 nm was developed. The effects of pH, concentration of buffer, and organic modifiers in the electrophoretic separation were investigated. The buffer used for CZE contained 50 mM ammonium acetate, pH 3.1. The effect of temperature on the extraction efficiency of strychnine in medicinal plants by PLE was demonstrated. Comparable or higher extraction efficiency was achieved with PLE for strychnine in medicinal plants and berberine in herbal preparations compared to soxhlet extraction. The effect of matrix interference in medicinal plants and herbal preparations containing a number of medicinal plants samples using CZE was investigated by standard additional experiments. The reproducibility of the method using PLE with CZE was found to vary between 2.4 and 10.7% (n = 5/6) for different types of samples on different days.

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Section: Introductionmentioning

confidence: 99%

Determination of berberine and strychnine in medicinal plants and herbal preparations by pressurized liquid extraction with capillary zone electrophoresis

Ong

Apandi

2001

Electrophoresis

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“…Instead, it has also served as a qualitative tool when coupled with a mass spectrometer (Buchberger et al ., ; Wu et al ., ). Much attention has been paid to classification of the plant material based on compound occurrence and concentration, namely, species fingerprinting (Li et al ., ; Hu et al ., ) and sample origin estimation (Reid et al ., ; Yu et al ., ; Ding et al ., ). Work of particular interest in the field has been presented by Reid et al .…”

Section: Applicationmentioning

confidence: 99%

The advances of electromigration techniques applied for alkaloid analysis

Dziomba

Belka

Kowalski

et al. 2013

Biomedical Chromatography

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Alkaloids are natural products of metabolism found mainly in plants. Their diversified pharmacological activities as medical agents and extensive occurrence in nutritional products demand the development of reliable and sensitive analytical tools. This review presents the developments in the field of electromigration techniques and specifically capillary and microchip electrophoresis. We include the main aspects of interest to researches over the past 12 years. The scope of this review covers detection, on- and off-line preconcentration techniques, chiral separation and developments in the field of microchip electrophoresis. Their applications in alkaloid determination, capillary electrochromatography and inter-molecular interactions are also examined.

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“…Most alkaloids have basic properties with pK a values ranging from 6 to 12 [47]. Thus, the donor phase should be sufficiently basic to facilitate the deprotonation of the alkaloids and consequently reduced their aqueous solubility.…”

Section: Composition Of Donor and Acceptor Phasementioning

confidence: 99%