Liquid Chromatographic Determination of the Constituents inShao-yao-tang and Related Chinese Herbal Preparations

Sheu, Shuenn‐Jyi; Li, Kwei-Lan

doi:10.1002/(sici)1521-4168(19981001)21:10<569::aid-jhrc569>3.0.co;2-r

Cited by 15 publications

(3 citation statements)

References 17 publications

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“…This is followed by multiple step extraction using chloroform with final analysis by TLC [3,4]. The content of glycyrrhizin was determined by HPLC [10,11]. In other reports, multiple step ultrasonic extractions using a mixture of methanol/water (70 : 30) and other solvents such as aqueous ethanol or methanol were used to extract glycyrrhizin present in Radix glycyrrhizae and other herbal preparations.…”

Section: Introductionmentioning

confidence: 99%

Chemical assay of glycyrrhizin in medicinal plants by pressurized liquid extraction (PLE) with capillary zone electrophoresis (CZE)

Ong¹

2002

J. Sep. Science

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Chemical assay of glycyrrhizin in medicinal plants by pressurized liquid extraction (PLE) with capillary zone electrophoresis (CZE)Glycyrrhizin in Radix glycyrrhizae or liquorice was determined using a laboratory made dynamic pressurized liquid extraction (PLE) system coupled with capillary zone electrophoresis (CZE). The amount of glycyrrhizin was determined using CZE with an aqueous buffer of 10 mM of sodium tetraborate at pH 9.25 with UV detection at 254 nm. The effects of pH, buffer concentration, and the applied voltage on the electrophoretic separation were investigated. Temperature was found to be the important parameter in PLE as it was observed that the amount of thermally labile components such as glycyrrhizin extracted from medicinal plants decreased with increasing temperature from 100 to 1608C. The amount of glycyrrhizin extracted by PLE with methanol as solvent for glycyrrhizin in medicinal plants was found to be comparable or higher compared to multiple step ultrasonic extraction using a mixture of methanol/ water (70 : 30). The overall method precision was found to vary from 6.9 to 8.1% (RSD) for glycyrrhizin in medicinal plants. The specificity of the method was checked using standard addition experiments. Figure 1. Chemical structures of A) 18b-glycyrrhetinic acid and B) glycyrrhizin.

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Section: Introductionmentioning

confidence: 99%

Chemical assay of glycyrrhizin in medicinal plants by pressurized liquid extraction (PLE) with capillary zone electrophoresis (CZE)

Ong¹

2002

J. Sep. Science

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“…1b) is found in medicinal plants such as Strychnos nuxvomica and Semen strychni pulveratum (maqianzi). Multiple steps of ultrasonic extraction were used to extract berberine in medicinal plants and related herbal preparations with analysis by HPLC and HPLC-MS, respectively [6][7][8]. Therapeutic dose was reported to produce a tonic effect on the alimentary canal, a limited amount of respiratory, and vasomotor stimulation.…”

Section: Introductionmentioning

confidence: 99%

Determination of berberine and strychnine in medicinal plants and herbal preparations by pressurized liquid extraction with capillary zone electrophoresis

Ong

Apandi

2001

Electrophoresis

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A simple and rapid method for the determination of berberine and strychnine in medicinal plants and herbal preparations for regulatory purposes using a home-made pressurized liquid extraction (PLE) system with capillary zone electrophoresis (CZE) using ultraviolet detection at 254 nm was developed. The effects of pH, concentration of buffer, and organic modifiers in the electrophoretic separation were investigated. The buffer used for CZE contained 50 mM ammonium acetate, pH 3.1. The effect of temperature on the extraction efficiency of strychnine in medicinal plants by PLE was demonstrated. Comparable or higher extraction efficiency was achieved with PLE for strychnine in medicinal plants and berberine in herbal preparations compared to soxhlet extraction. The effect of matrix interference in medicinal plants and herbal preparations containing a number of medicinal plants samples using CZE was investigated by standard additional experiments. The reproducibility of the method using PLE with CZE was found to vary between 2.4 and 10.7% (n = 5/6) for different types of samples on different days.

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“…In terms of gradient elution, it is the reverse case of isocratic analysis as the mobile composition changes overtime. Gradient elution with reverse-phase is the most commonly used in the HPLC analysis of multiple constituent present in herbal preparation or medicinal plants [184–189]. …”

Section: High Performance Liquid Chromatography (Hplc)mentioning

confidence: 99%

Review: DNA Barcoding and Chromatography Fingerprints for the Authentication of Botanicals in Herbal Medicinal Products

Abubakar

Salleh²,

Shamsir³

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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In the last two decades, there has been a tremendous increase in the global use of herbal medicinal products (HMPs) due to their claimed health benefits. This has led to increase in their demand and consequently, also, resulted in massive adulteration. This is due to the fact that most of the traditional methods cannot identify closely related species in a process product form. Therefore the urgent need for simple and rapid identification methods resulted in the discovery of a novel technique. DNA barcoding is a process that uses short DNA sequence from the standard genome for species identification. This technique is reliable and is not affected by external factors such as climates, age, or plant part. The difficulties in isolation of DNA of high quality in addition to other factors are among the challenges encountered using the DNA barcoding in the authentication of HMP. These limitations indicated that using DNA barcoding alone may ineffectively authenticate the HMP. Therefore, the combination of DNA barcoding with chromatographic fingerprint, a popular and generally accepted technique for the assessment and quality control of HMP, will offer an efficient solution to effectively evaluate the authenticity and quality consistency of HMP. Detailed and quality information about the main composition of the HMPs will help to ascertain their efficacy and safety as these are very important for quality control.

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Liquid Chromatographic Determination of the Constituents inShao-yao-tang and Related Chinese Herbal Preparations

Cited by 15 publications

References 17 publications

Chemical assay of glycyrrhizin in medicinal plants by pressurized liquid extraction (PLE) with capillary zone electrophoresis (CZE)

Chemical assay of glycyrrhizin in medicinal plants by pressurized liquid extraction (PLE) with capillary zone electrophoresis (CZE)

Determination of berberine and strychnine in medicinal plants and herbal preparations by pressurized liquid extraction with capillary zone electrophoresis

Review: DNA Barcoding and Chromatography Fingerprints for the Authentication of Botanicals in Herbal Medicinal Products

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