Liquid chromatographic determination of vitamins B1 and B2 in foods. A collaborative study

Arella, F; Lahély, S; Bourguignon, J.B.; Hasselmann, C.

doi:10.1016/0308-8146(95)00149-2

Cited by 83 publications

(43 citation statements)

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“…Due to the extensive phosphorylated and protein-bound vitamins in natural foods, quantitative analysis of the entire array of B-group vitamins at endogenous levels requires vigorous sample extraction to release the free vitamins using strong acids and enzyme hydrolysis. [19][20][21][22][23][24][25][26][27][28][29][30] However, these tedious, complex and harsh methods are not necessary to extract the vitamins from vitamin-supplemented food products.…”

Section: Resultsmentioning

confidence: 99%

“…[19][20][21][22]24,25,27,30 Interference in the chromatograms and poor recovery indicate degradation of some vitamins due to the harsh acid. Organic solvents can also be used to precipitate proteins and are milder toward the samples.…”

Section: Resultsmentioning

confidence: 99%

“…Extraction techniques based on different acid treatments and a These authors contributed equally to this work. enzymatic digestion such as hydrochloric acid, sulfuric acid, perchloric acid or amylase and taka-diastase [19][20][21][22][23][24][25][26][27][28][29][30] have been developed, but the acids or enzymes require a long incubation (normally over 10-h) and high heat. Thus, these processes may decrease the recovery of unstable vitamins, such as folic acid.…”

Section: -17mentioning

confidence: 99%

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Simultaneous Determination of B Group Vitamins in Supplemented Food Products by High Performance Liquid Chromatography-Diode Array Detection

Suh¹,

Yang²,

Lee³

et al. 2011

Bulletin of the Korean Chemical Society

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A simple HPLC-DAD method was developed and validated to determine B group vitamin content (thiamine, riboflavin, nicotinamide, pantothenic acid, pyridoxine and folic acid) in supplemented food samples, i.e., infant formula, cereal, low-calorie food, a multi-vitamin pill and a vitamin drink. In this study, the most significant advantages were simultaneous determination of the six B group vitamins in various food matrices and a small number of sample treatment steps that required only an organic solvent, acetonitrile. Moreover, this method prevents reduction of column durability, because the mobile phase does not contain ion-pairing reagents. Analytes were separated on a Develosil RPAQUEOUS C 30 (4.6 mm × 250 mm, 5 µm particle size) column with a gradient elution of acetonitrile and 20 mM phosphate buffer (pH 3.0) at a flow rate between 0.8 and 1.0 mL/min. Detection was performed at 275 nm, except for that of pantothenic acid (205 nm). The calibration curves for all six vitamins showed good linearity with correlation coefficients (r 2 ) higher than 0.995. The developed method was validated with respect to linearity, intra-and inter-day accuracy and precision, limit of quantification (LOQ), recovery and stability. The method showed good precision and accuracy, with intra-and inter-assay coefficients of variation less than 15% at all concentrations. The recovery was carried out according to the standard addition procedure, with yields ranging from 89.8 to 104.4%. This method was successfully applied to the determination of vitamin B groups in supplemented food products.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: -17mentioning

confidence: 99%

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Simultaneous Determination of B Group Vitamins in Supplemented Food Products by High Performance Liquid Chromatography-Diode Array Detection

Suh¹,

Yang²,

Lee³

et al. 2011

Bulletin of the Korean Chemical Society

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“…A literatura apresenta métodos analí-ticos para determinação das vitaminas nesses produtos como, por ex., a determinação das vitaminas do complexo B pode ser realizada através de métodos microbiológicos 13,14 , CLAE [15][16][17][18][19][20][21][22] , cromatografia micelar eletrocinética 23 , cromatografia gasosa 24 e por espectrofotometria, empregando sistema de análises por injeção em fluxo 25 . Entretanto, a literatura é deficiente em relação à avaliação dos níveis e da estabilidade das vitaminas em bebidas fortificadas.…”

Section: Introductionunclassified

Avaliação do teor e da estabilidade de vitaminas do complexo B e vitamina C em bebidas isotônicas e energéticas

Castro¹,

Scherer²,

Godoy³

2006

Quím. Nova

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) in power beverages. The amount of vitamins was found to be above of that declared on the labels, even after the shelf life had been exceeded. A small decrease in the amount of B group vitamins was observed during the shelf life of the products. In the case of vitamin C this decrease was slightly higher. The present research shows the need of increased quality control and inspection.Keywords: HPLC; pyridoxine; ascorbic acid. INTRODUÇÃOBebidas isotônicas são repositores hidrolíticos formulados a partir da concentração variada de eletrólitos, associada a concentrações variadas de carboidratos, com o objetivo de reposição hídrica e eletrolítica decorrentes da prática de atividade física 1 . Esses produtos, segundo a ANVISA 1 (Agência Nacional de Vigilância Sanitária), devem apresentar concentrações variadas de sódio, cloreto e carboidratos. Opcionalmente, podem conter potássio, vitaminas e/ou minerais em concentrações semelhantes às encontradas nos fluidos orgânicos. Por outro lado, os repositores energéticos são produtos formulados com nutrientes que permitam o alcance e/ou manutenção do nível apropriado de energia para atletas. São produtos nos quais os carboidratos devem constituir, no mínimo, 90% dos nutrientes energéticos presentes na formulação. Opcionalmente, estes produtos podem conter vitaminas e/ou minerais 1 , ou ainda cafeína e alguns aminoácidos.A fortificação de bebidas isotônicas e energéticas com vitaminas tem sido uma prática adotada pela maioria das indústrias do ramo, sendo as vitaminas do complexo B, vitaminas C e ácido fólico as principais encontradas nos produtos. Vitaminas lipossolúveis, como A e E, também podem ser encontradas pelo uso de microencapsulação. Além da função repositória após exercícios físicos, as bebidas fortificadas podem ser utilizadas para outros objetivos, como por ex., para controlar deficiências vitamínicas em comunidades carentes, além de prevenir doenças.Um estudo realizado no sul da Espanha revelou que a população local consome de 2 a 3 vezes abaixo dos níveis recomendados para as vitaminas B 6 , B 12 e folatos 2 . Abrams et al. 3 avaliaram a eficácia de bebidas fortificadas com vitaminas em crianças de 6 a 11 anos em relação os níveis séricos de vitaminas, durante 8 semanas. Os autores citam que os níveis séricos de riboflavina e ácido fólico aumentaram significativamente e, ainda, concluem que a fortificação de bebidas para populações de risco pode ser uma alternativa viável para controle de deficiência vitamínica, devido à fácil aceitação do produto. Sichert-Hellert e Kersting 4 relatam que o consumo de vitaminas B 1 , B 2 , B 3 , B 6 , A, E, C e ácido fólico aumentou entre jovens e adolescentes na Alemanha devido ao consumo de bebidas fortificadas, como isotônicos e energéticos. Rimm et al. 5 relatam que o risco de doenças coronárias foi reduzido em mulheres com consumo regular de vitamina B 6 e ácido fólico. Zhang et al. 6 relatam uma associação inversa altamente significativa entre os níveis plasmáticos de folatos e vitamina B 12 com o risco de câncer de mama. ...

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“…10,13,[15][16][17] Ion-pairing reagents improve the chromatographic resolution and peak shape, particularly by reducing peak tailing. With these methods, the mixture of an acetate buffer with 1-octanesulfonate and organic solvents (acetonitrile and methanol) was selected as the mobile phase for the pharmaceutical analysis of thiamine in both United States Pharmacopoeia (USP) 18 and Korean Pharmacopoeia (KP) 19 monographs.…”

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confidence: 99%

Determination of Thiamine in Pharmaceutical Preparations by Reverse Phase Liquid Chromatography Without Use of Organic Solvent

Suh¹,

Kim²,

Jung³

et al. 2013

Bulletin of the Korean Chemical Society

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A novel green aqueous mobile phase modified with room temperature ionic liquids (RTILs) was employed in the absence of volatile organic solvents or ion-pairing reagents to analyze thiamine, a very polar compound, by reverse phase high performance liquid chromatography (RP-HPLC). Due to its strongly hydrophilic nature, thiamine was eluted near the column dead time (t 0 ) using a mobile phase without adding RTILs or ion-pairing reagents, even if a 100% aqueous mobile phase, which has weak elution power under reverse phase conditions, was used. Thus, 1-ethyl-3-methyl-imidazolium hexafluorophosphate ([EMIM][PF 6 ]), which has the strongest chaotropic effect, was selected as a mobile phase additive to improve retention and avoid baseline disturbances at t 0 . Various mobile phase parameters such as cation moiety, chaotropic anion moiety, pH and concentration of RTILs were optimized to determine thiamine at the proper retention time. Method validation was performed to assess linearity, intra-and inter-day accuracy and precision, recovery and repeatability; all results were found to be satisfactory. The developed method was also compared to the current official United States Pharmacopoeia (USP) and Korean Pharmacopoeia (KP) methods using an organic mobile phase containing an ionpairing reagent by means of evaluating various chromatographic parameters such as the capacity factor, theoretical plate number, peak asymmetry and tailing factor. The results indicated that the proposed method exhibited better efficiency of thiamine analysis than the official methods, and it was successfully applied to quantify thiamine in pharmaceutical preparations.

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Liquid chromatographic determination of vitamins B1 and B2 in foods. A collaborative study

Cited by 83 publications

References 1 publication

Simultaneous Determination of B Group Vitamins in Supplemented Food Products by High Performance Liquid Chromatography-Diode Array Detection

Simultaneous Determination of B Group Vitamins in Supplemented Food Products by High Performance Liquid Chromatography-Diode Array Detection

Avaliação do teor e da estabilidade de vitaminas do complexo B e vitamina C em bebidas isotônicas e energéticas

Determination of Thiamine in Pharmaceutical Preparations by Reverse Phase Liquid Chromatography Without Use of Organic Solvent

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