Liquid-chromatographic measurement of biopterin and neopterin in serum and urine

Slazyk, W E; Spierto, F. W.

doi:10.1093/clinchem/36.7.1364

Cited by 28 publications

(5 citation statements)

References 10 publications

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“…10 Differential diagnosis is most commonly performed by analysis of urinary neopterin and biopterin. 11 Quantication of urinary neopterin and biopterin has been successfully performed by high-performance liquid chromatography (HPLC) coupled with electrochemical measurements, uorescence or MS. [12][13][14][15] Due to the complicated matrix, extensive sample pre-treatment, including extraction, pre-concentration and chromatographic separation, etc., which can take tens of minutes or even hours, is usually required. The cost of consumables, such as consumable kits for the extraction of samples, and solvents for sample extractions and chromatographic separations, is also relatively high.…”

Section: Rapid Assay Of Neopterin and Biopterin In Urine By Wooden-ti...mentioning

confidence: 99%

Rapid assay of neopterin and biopterin in urine by wooden-tip electrospray ionization mass spectrometry

et al. 2015

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Section: Rapid Assay Of Neopterin and Biopterin In Urine By Wooden-ti...mentioning

confidence: 99%

Rapid assay of neopterin and biopterin in urine by wooden-tip electrospray ionization mass spectrometry

et al. 2015

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“…Recently, a UPLC-MS/MS-based method has also been described by [124]. Analysis of pterin levels in human plasma and serum usually involves oxidation with either MnO 2 or differential oxidation with iodide/iodine after a filtration step [121]. Recently, a new protocol containing a derivatization step for the precise measurement of BH 4 has been published by [119].…”

Section: Diagnostic Methodsmentioning

confidence: 99%

Analysis of Catecholamines and Pterins in Inborn Errors of Monoamine Neurotransmitter Metabolism—From Past to Future

Jung-Klawitter

Hübschmann

2019

Cells

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Inborn errors of monoamine neurotransmitter biosynthesis and degradation belong to the rare inborn errors of metabolism. They are caused by monogenic variants in the genes encoding the proteins involved in (1) neurotransmitter biosynthesis (like tyrosine hydroxylase (TH) and aromatic amino acid decarboxylase (AADC)), (2) in tetrahydrobiopterin (BH4) cofactor biosynthesis (GTP cyclohydrolase 1 (GTPCH), 6-pyruvoyl-tetrahydropterin synthase (PTPS), sepiapterin reductase (SPR)) and recycling (pterin-4a-carbinolamine dehydratase (PCD), dihydropteridine reductase (DHPR)), or (3) in co-chaperones (DNAJC12). Clinically, they present early during childhood with a lack of monoamine neurotransmitters, especially dopamine and its products norepinephrine and epinephrine. Classical symptoms include autonomous dysregulations, hypotonia, movement disorders, and developmental delay. Therapy is predominantly based on supplementation of missing cofactors or neurotransmitter precursors. However, diagnosis is difficult and is predominantly based on quantitative detection of neurotransmitters, cofactors, and precursors in cerebrospinal fluid (CSF), urine, and blood. This review aims at summarizing the diverse analytical tools routinely used for diagnosis to determine quantitatively the amounts of neurotransmitters and cofactors in the different types of samples used to identify patients suffering from these rare diseases.

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“…The mean value obtained in healthy controls is 0.55 =k 0.14 IU mL-I (manufacturer's information). Determination of neopterin was by reversed-phase high-performance liquid chromatography with minor modifications [9]. Briefly, urine samples were centrifuged to remove debris, diluted in 1 to 10 with water containing dimethylpterine as an internal standard and injected directly onto a Techsphere 5 ODS column (HPLC Technology Ltd).…”

Section: Laboratory Measurementsmentioning

confidence: 99%

Interferon‐γ and urine neopterin in attacks of the hyperimmunoglobulinaemia D and periodic fever syndrome

Drenth

Powell

Brown

et al. 1995

Eur J Clin Investigation

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Abstract. The hyperimmunoglobulinaemia D and periodic fever (hyper-IgD) syndrome is typified by recurrent unpredictable febrile attacks with abdominal pain, joint involvement (arthralgias/arthritis), headache, skin lesions and a polyclonal elevation of serum IgD ( > 100 U mL-'). Interferon-gamma (IFN-y) is a major proinflammatory cytokine which could play a role in the pathogenesis of the attacks. There is a need for parameters (if possible non-invasive) to monitor disease activity. A potential candidate is neopterin which is released by monocytes/macrophages when stimulated with IFN-y, excreted unchanged in urine, and appears to be an early and sensitive marker for activation of the immune system. We measured rectal body temperature, serum IFN-y, and urine neopterin in 10 hyper-IgD patients both during and between attacks. The body temperature rose to a mean of 38.9"C on the first day of the attack and normalized within 5 days. Serum IFN-y during the first day of the attack was 2.98 IU mL-' and was significantly lower during remissions. The urine neopterin excretion was 268 f 170 pmolmol-' creatinine between attacks and was significantly increased to 638 f 275 pmolmol-' creatinine on the first day of symptoms. Maximal urine neopterin values were reached on the fourth day of the attack (1051 f 387 pmol mol-' creatinine) and excretion gradually declined and attained values below 400 pmol mol-' creatinine after 9 days. There was a good correlation between serum IFN-y and urine neopterin. The increases in serum IFN-y and urine neopterin suggest activation of the cellular immunity during the febrile attacks of the hyper-IgD syndrome. Furthermore, the activation of the cellular immune system appears to persist several days after normalization of the body temperature. The significant correlation between IFN-y and urine neopterin in the hyper-IgD syndrome accords with experimental data suggesting that IFN-y is the dominant factor in the release of neopterin. Our study shows that urinary neopterin is a good quantitative and qualitative parameter to monitor disease activity in patients with the hyper-IgD syndrome

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Liquid-chromatographic measurement of biopterin and neopterin in serum and urine

Cited by 28 publications

References 10 publications

Rapid assay of neopterin and biopterin in urine by wooden-tip electrospray ionization mass spectrometry

Rapid assay of neopterin and biopterin in urine by wooden-tip electrospray ionization mass spectrometry

Analysis of Catecholamines and Pterins in Inborn Errors of Monoamine Neurotransmitter Metabolism—From Past to Future

Interferon‐γ and urine neopterin in attacks of the hyperimmunoglobulinaemia D and periodic fever syndrome

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