Rapid assay of neopterin and biopterin in urine by wooden-tip electrospray ionization mass spectrometry

Yang, Bicheng; Liu, Faying; Guo, Jiubai; Wan, Lei; Wu, Juan; Wang, Feng; Liu, Huai; Huang, Ouping

doi:10.1039/c5ay00004a

Cited by 17 publications

(20 citation statements)

References 29 publications

(33 reference statements)

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“…According to the previous studies and our experimental investigation, 11,[30][31][32] in general, when wooden tips were used for sample loading and ionization, no signicant background noise and interfering mass peaks were observed, probably because the wooden tip is an inert material. The wooden toothpicks (purchased from the Nanchang supermarket) were manufactured by SunChua wood and bamboo company (Zhejiang, China).…”

Section: Wooden-tip Esi-ms Analysissupporting

confidence: 58%

Serum metabolic profiling study of endometriosis by using wooden-tip electrospray ionization mass spectrometry

et al. 2015

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A high throughput metabolite fingerprinting tool based on wooden-tip electrospray ionization mass spectrometry (WT-ESI-MS) has been established for the serum metabolic profiling study of endometriosis with little sample pre-treatment, no chromatography and instrument cycle times of less than 5 min. Serum samples from endometriosis patients and healthy controls were analyzed by direct WT-ESI-MS with a high resolution ESI-Q-TOF-MS. The resulting data were analyzed by multivariate data analysis. MS/MS experiments were carried out to identify potential biomarkers. Global metabolic profiling and subsequent multivariate analysis clearly distinguished endometriosis patients from healthy controls.A total of ten metabolites, up-regulated or down-regulated, were identified which contribute to the progress of endometriosis. These promising identified biomarkers underpin the metabolic pathway including steroid hormone biosynthesis, glycerophospholipid metabolism, sphingolipid metabolism, pyruvate metabolism, bile acid biosynthesis, and androgen and estrogen metabolisms. Considering that a much higher throughput can be obtained without a chromatographic step, the present WT-ESI-MS method could be developed as a fast prognostic or diagnostic method for endometriosis.

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Section: Wooden-tip Esi-ms Analysissupporting

confidence: 58%

Serum metabolic profiling study of endometriosis by using wooden-tip electrospray ionization mass spectrometry

et al. 2015

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“…Since 2009, liquid chromatography coupled to tandem MS was used to detect N and B in plasma [30], as well as for the simultaneous detection of BH4, BH2, and B in urine samples, cell extracts, and rat brains [31][32][33][34]. However, according to the authors, MS/MS detection is less sensitive than FD for pterins quantification [31].…”

Section: Hplc-ms Methodsmentioning

confidence: 99%

Pterin determination in cerebrospinal fluid: state of the art

Guibal

Maître

et al. 2017

Pteridines

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AbstractThe analysis of pterins in the cerebrospinal fluid (CSF) is mandatory for the etiologic diagnosis of inborn errors of dopamine and serotonin metabolism. The success of the available therapeutic strategies for preventing the ongoing brain dysfunction is tightly dependent of the early diagnosis of these neurotransmitter disorders. Previous methods of pterins determination in the CSF have in common at least one reversed phase HPLC step coupled to electrochemical or fluorescence detection (FD). They differ in the oxidation procedure of the reduced forms of pterins into their oxidized fluorescent counterparts. Most of the methods using the FD include at least one offline chemical oxidation procedure and cannot allow the direct quantification of tetrahydrobiopterin (BH4). A recent method proposed a single step simultaneous quantification of all forms of pterins including BH4 by HPLC coupled to FD after post-column coulometric oxidation. Nowadays, recent advances in mass spectrometry (MS), notably in term of sensitivity, allow the direct unambiguous determination of all forms of pterins in the CSF by LC-MS/MS.

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“…19,25,26 For sampling of the liquid samples (i.e., soup, beverage) and viscous sample (i.e., residual drink), 1.0 cm of the dry wooden tip was directly immersed in the sample for about 10 seconds, then about 10 mL of the sample was absorbed by the porous wooden tips (which was calculated by original volume minus the volume aer sampling). 19,25,26 For sampling of the liquid samples (i.e., soup, beverage) and viscous sample (i.e., residual drink), 1.0 cm of the dry wooden tip was directly immersed in the sample for about 10 seconds, then about 10 mL of the sample was absorbed by the porous wooden tips (which was calculated by original volume minus the volume aer sampling).…”

Section: Wooden-tip Samplingmentioning

confidence: 99%

“…[12][13][14] The development of ESI techniques with solid substrates has taken place since ESI with copper wires was developed in the late 1990s. ESI using wooden tips (common toothpicks) is readily available with very low costs and can be directly mounted on a nanoESI device without hardware modication, 18 showing that wooden-tips could be used for many applications due to their convenient sampling and rapid analysis, e.g., pharmaceutical analysis, 23 metabolite analysis, 24,25 forensic analysis, 26 and plant identication. ESI using wooden tips (common toothpicks) is readily available with very low costs and can be directly mounted on a nanoESI device without hardware modication, 18 showing that wooden-tips could be used for many applications due to their convenient sampling and rapid analysis, e.g., pharmaceutical analysis, 23 metabolite analysis, 24,25 forensic analysis, 26 and plant identication.…”

Section: Introductionmentioning

confidence: 99%

Wooden-tip electrospray ionization mass spectrometry for trace analysis of toxic and hazardous compounds in food samples

et al. 2015

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Wooden-tip electrospray ionization (wooden-tip ESI) is applied to the rapid, in situ, direct qualitative and quantitative trace analysis of toxic and hazardous compounds in food samples. To evaluate the potential of wooden-tip ESI mass spectrometry (MS) in food analysis, pesticides, toxicants, date-rape drugs, and illicit additives were detected in various food samples. Wooden-tip ESI-MS experiments were performed using wooden tip sampling and ionization, with examination using MS and tandem mass spectrometry (MS/MS). The results proved that wooden-tip ESI allows the detection and confirmation of traces of toxic and hazardous compounds in food. In addition, selected analytes in beverages were obtained at absolute levels as low as $10 pg. Quantitation of analytes in liquid and powder samples was also evaluated. Single sample analysis was completed within 2 min. The data obtained shows that the wooden-tip ESI-MS is a promising tool for rapid analysis of food samples.

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Rapid assay of neopterin and biopterin in urine by wooden-tip electrospray ionization mass spectrometry

Abstract: Rapid detection and quantitation of neopterin and biopterin in clinical urine by wooden-tip electrospray ionization mass spectrometry.

Cited by 17 publications

References 29 publications

Serum metabolic profiling study of endometriosis by using wooden-tip electrospray ionization mass spectrometry

Serum metabolic profiling study of endometriosis by using wooden-tip electrospray ionization mass spectrometry

Pterin determination in cerebrospinal fluid: state of the art

Wooden-tip electrospray ionization mass spectrometry for trace analysis of toxic and hazardous compounds in food samples

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