Liquid-chromatographic profiling of solutes in serum of uremic patients undergoing hemodialysis and chronic ambulatory peritoneal dialysis (CAPD); high concentrations of pseudouridine in CAPD patients.

Schoots, A.C.; Gerlag, P. G. G.; Mulder, Abbey; Peeters, J.A.G.; Cramers, C.A.M.G.

doi:10.1093/clinchem/34.1.91

Cited by 22 publications

(9 citation statements)

References 3 publications

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“…Pseudouridine (⌿), 1-methyladenosine (m 1 A), 1-methylinosine (m 1 I), 1-methylguanosine (m 1 G), N 4 -acetylcytidine (ac 4 C), N 2methylguanosine (m 2 G), adenosine (A), N 6 -methyladenosine (m 6 A), allopurinol ribonucleoside (APR), 3-methyluridine (m 3 U) and 5-bromouridine (br 5 U) were obtained from Sigma Chemical Co. (St. Louis, MO, USA). Uridine (U), guanosine (G) and cytidine (C) were obtained from Kohjin Co. (Tokyo, Japan), and xanthosine (X) from Nakarai Chemical Co. (Kyoto, Japan).…”

Section: Chemicalsmentioning

confidence: 99%

“…Pseudouridine derived predominantly from tRNA is not catabolized but excreted in urine as the intact molecule. The serum level of pseudouridine has been reported to be increased in uremic patients [3][4][5][6], especially when they are undergoing continuous ambulatory peritoneal dialysis (CAPD) [4]. Further analytical study on the metabolism of modified ribonucleosides in uremic patients is required to shed light on their possible roles as uremic toxins.…”

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confidence: 99%

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RNA metabolism in uremic patients: Accumulation of modified ribonucleosides in uremic serum

Niwa¹,

Takeda²,

Yoshizumi³

1998

Kidney International

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To determine the metabolism of ribonucleic acid (RNA) in uremia, serum and urine levels of ribonucleosides in uremic patients were analyzed using reversed-phase high-performance liquid chromatography. The serum levels of xanthosine and all modified ribonucleosides were increased in undialyzed patients with chronic renal failure (CRF), and patients undergoing hemodialysis (HD) and continuous ambulatory peritoneal dialysis (CAPD). The serum level of pseudouridine was markedly increased in all the uremic patients especially CAPD patients (32 times higher than normal). By contrast, the serum level of adenosine did not show any significant change in the uremic patients. Interestingly, the serum and urine levels of inosine were significantly decreased in all the uremic patients, suggesting that the production of inosine is decreased in uremic patients. The serum level of uridine was significantly elevated only in the HD patients. The serum levels of all ribonucleosides except inosine and uridine decreased significantly after HD. The urinary excretion of inosine, 1-methyladenosine, 1-methylguanosine, N2,N2-dimethylguanosine and N4-acetylcytidine was significantly decreased in the CRF patients, leading to the accumulation of these modified ribonucleosides in the uremic serum. CAPD patients showed markedly increased serum levels of modified ribonucleosides such as pseudouridine, 1-methylinosine, and N2,N2-dimethylguanosine and N4-acetylcytidine as compared with the HD patients. These results demonstrate that there was an altered metabolism of RNA in uremic patients with marked accumulation of modified ribonucleosides.

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Section: Chemicalsmentioning

confidence: 99%

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confidence: 99%

RNA metabolism in uremic patients: Accumulation of modified ribonucleosides in uremic serum

Niwa¹,

Takeda²,

Yoshizumi³

1998

Kidney International

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“…HPLC. This was performed as described previously (10,11). A column of "Ultrasphere Octyl" (C8-modifled silica), 4.6 mm (i.d.)…”

Section: Methodsmentioning

confidence: 99%

“…Twentytwo characteristic uremic solutes in sera of dialyzed and non-dialyzed uremic patients were subjected to principal component analysis, in order to explore interdependencies between these concentrations and their distribution in different patients. These ultraviolet-absorbing and (or) fluorescent solutes were analyzed by gradient-elution high-performance liquid chromatography (HPLC) as described previously (10,11).…”

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Are the classical markers sufficient to describe uremic solute accumulation in dialyzed patients? Hippurates reconsidered.

et al. 1988

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Interdependencies of accumulated solutes, analyzed by liquid chromatography in dialyzed and non-dialyzed patients, were studied by multivariate statistical analysis. In principal component analysis, three principal components (PC1-PC3) were retained from the data on 22 accumulated compounds in dialyzed patients, whereas only one principal component was retained from analogous data of a non-dialyzed patient group. PC1 in the dialyzed patient group comprises concentrations of hippuric acid, p-hydroxyhippuric acid, tryptophan, and five unidentified fluorescent solutes in serum. Concentrations of the classical markers urea, uric acid, creatinine, and phosphate were closely related to PC2 in these patients. Indoleacetic acid and two unidentified fluorescent compounds constitute PC3. The compounds associated with the groups found by principal component analysis may be characterized by chemical structure and by the mechanism of their excretion via the remaining nephrons of dialyzed patients. In the non-dialyzed group, most of the solutes could be described by a single PC. This PC and PC1 from the dialyzed group correlated significantly with residual renal function, and with total ultraviolet absorbance and total fluorescence emission. The data suggest that it is of value to introduce a marker of uremic solute retention in addition to urea, to account for renal-function-related "organic-acid-like" compounds that are excreted by renal tubular secretion in dialyzed patients. The hippurates may serve this purpose.

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“…Organic acids in uremic serum can be analyzed, as a group, by use of temperature-programmed gas chromatography (17,18) or "high-performance" liquid chromatography (HPLC) with gradient-elution (9,(19)(20)(21), both of which are relatively time-consuming procedures. Specific analysis for HA has also been done colorimetrically (22) in the injected calibration solutions of 0, 12.5, 25, 50, and 125 p.moIJL for CZE analysis and 0, 25, 50, 100, and 250 p.moliL for HPLC analysis.…”

Section: Renal Function Tubular Secretion Anion Gap Uremic Toxins Hplc Compared Solvent Effectmentioning

confidence: 99%

Ultraviolet-absorbing organic anions in uremic serum separated by capillary zone electrophoresis, and quantification of hippuric acid

et al. 1990

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Organic anions accumulated in blood serum of patients with chronic renal failure were separated by a novel technique: closed-system capillary zone electrophoresis (CZE) in a pH6 carrier-electrolyte system. Hippuric acid (HA), p-hydroxyhippuric acid, and uric acid were identified by their co-elution with standards prepared in ultrafiltered normal serum and by comparison with the corresponding ultraviolet-detected peaks positively identified in the HPLC analyses. Analysis time for the entire profile is 8 min. Repeatabilities (CVs) of CZE migration times and peak areas of the three acids in serum samples were about 0.7% and 6%, respectively. We quantified HA in 10 ultrafiltered uremic serum samples and compared results with those by a previously described HPLC procedure. The very good agreement further supports the identification of hippuric acid. Accuracy and precision of the CZE method were similar to those for the HPLC gradient-elution method, but analysis time for HA (8 min) is much less than by HPLC (90 min). Our technique is very suitable for selective, rapid analysis for (ultraviolet-absorbing) anionic constituents in ultrafiltered uremic serum, without any sample pretreatment.

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Liquid-chromatographic profiling of solutes in serum of uremic patients undergoing hemodialysis and chronic ambulatory peritoneal dialysis (CAPD); high concentrations of pseudouridine in CAPD patients.

Cited by 22 publications

References 3 publications

RNA metabolism in uremic patients: Accumulation of modified ribonucleosides in uremic serum

RNA metabolism in uremic patients: Accumulation of modified ribonucleosides in uremic serum

Are the classical markers sufficient to describe uremic solute accumulation in dialyzed patients? Hippurates reconsidered.

Ultraviolet-absorbing organic anions in uremic serum separated by capillary zone electrophoresis, and quantification of hippuric acid

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