Liquid chromatographic-thermospray tandem mass spectrometric quantitative analysis of some drugs with hypnotic. sedative and tranquillising properties in whole blood

Verweij, Anthonie M.A.; Hordijk, Maarten L.; Lipman, Peter J.L.

doi:10.1016/s0378-4347(96)00035-7

Cited by 42 publications

(14 citation statements)

References 23 publications

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“…However, high-performance liquid chromatography with mass spectrometric detection is becoming increasingly the method of choice for simultaneous quantification and confirmation of many polar and/or chemically instable drugs, including benzodiazepines [13][14][15][16][17]. The analysis of benzodiazepines can be performed by using APCI-LC-MS [7,18,19] as well as electro spray ionization (ESI)-LC-MS [20,21].…”

Section: Discussionmentioning

confidence: 99%

Quantitative analysis of 33 benzodiazepines, metabolites and benzodiazepine-like substances in whole blood by liquid chromatography?(tandem) mass spectrometry

Smink

BRANDSMA

Dijkhuizen

et al. 2004

Journal of Chromatography B

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A quantitative method using high-performance liquid chromatography-mass spectrometry (LC-MS, ion trap) after matrix supported liquid-liquid extraction is described for the simultaneous determination in whole blood of 33 benzodiazepines including metabolites and benzodiazepine-like substances. The limits of detection (LOD) range from 0.0001 to 0.0126 mg/l. Linearity is satisfactory for all compounds. The extraction recoveries for the benzodiazepines in whole blood are between 60 and 91%, desmethyldiazepam, OH-bromazepam and brotizolam excepted. Selectivity, accuracy and precision are satisfactory for clinical and forensic purposes.

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Section: Discussionmentioning

confidence: 99%

Quantitative analysis of 33 benzodiazepines, metabolites and benzodiazepine-like substances in whole blood by liquid chromatography?(tandem) mass spectrometry

Smink

BRANDSMA

Dijkhuizen

et al. 2004

Journal of Chromatography B

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“…The determination of DZP and its metabolites in plasma, brain and cerebrospinal fluid (CSF) would have a significant impact on the study of treatments for various neurological disorders. Several analytical techniques have been published for the determination of DZP and its metabolites, namely high‐performance liquid chromatography (HPLC‐UV),3–11 gas chromatography (GC),12 gas chromatography/mass spectrometry (GC/MS),13–15 and liquid chromatography/mass spectrometry (LC/MS, LC/MS‐MS) 16,17. Most of the biological matrices assayed using these published methods are plasma, serum, blood, and urine from various animal species.…”

mentioning

confidence: 99%

Simple and sensitive liquid chromatography/tandem mass spectrometry method for the determination of diazepam and its major metabolites in rat cerebrospinal fluid

Wang

Shen

Fenyk-Melody

et al. 2003

Rapid Comm Mass Spectrometry

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Diazepam (DZP) is one of the most commonly prescribed drugs for treating status epilepticus (SE). A simple, sensitive and selective LC/MS/MS method with a wide linear calibration range was developed to quantify DZP and its major metabolites, N-desmethyldiazepam (DMDZP), temazepam (TZP), and oxazepam (OZP), in rat cerebrospinal fluid (CSF). The method was used to simultaneously determine the concentrations of all analytes in a small sample volume (as little as 25 microL) of rat CSF. The lower limits of quantification (LLOQ) of the method are 0.04 ng/mL for DZP and 0.1 ng/mL for its metabolites. The calibration range is 0.04-200 ng/mL for DZP and 0.1-200 ng/ml for the metabolites. All intra- and inter-assay coefficients of variation (%CV) and mean percent errors of the method are less than 12%. This method successfully addresses the need to determine low therapeutic drug concentrations in small physiological samples, namely rat CSF. Moreover, it can be used to investigate the distribution of the drug and its metabolites among blood plasma, brain tissue, and CSF in pharmacokinetic and pharmacodynamic studies in a variety of laboratory animals. With respect to animal experiments involving assays in CSF, this method addresses two of the three criteria of Russell and Bruch (Principles of Humane Experimental Techniques, 1959, Methuen and Co., London) for minimizing animal use, namely refinement and reduction.

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“…In fine HPLC, a method of increasing importance for these purposes is being used with ultraviolet, fluorescence or electrochemical detection and even with MS applying soft ionization interfaces like thermospray ionization (TSP), electrospray ionization (ESI), sonic spray ionization (SSI), atmospheric pressure chemical ionization (APCI) or photoionization (APPI). The application of HPLC-MS-MS for qualitative and quantitative analysis of benzodiazepines has been described more recently [38,[59][60][61]. MS modes; electrospray ionization (ESI), multiple reaction monitoring (MRM), information dependent acquisition (IDA), enhanced product ion scan (EPI) were compared [62] for detection and quantification of 21 benzodiazepines in human hair samples.…”

Section: High Performance Liquid Chromatography (Hplc)mentioning

confidence: 99%

An Overview on Total Analytical Methods for the Detection of 1,4-Benzodiazepines

Uddin¹,

Victoria²,

Samanidou³

et al. 2014

Pharm Anal Acta

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Liquid chromatographic-thermospray tandem mass spectrometric quantitative analysis of some drugs with hypnotic. sedative and tranquillising properties in whole blood

Cited by 42 publications

References 23 publications

Quantitative analysis of 33 benzodiazepines, metabolites and benzodiazepine-like substances in whole blood by liquid chromatography?(tandem) mass spectrometry

Quantitative analysis of 33 benzodiazepines, metabolites and benzodiazepine-like substances in whole blood by liquid chromatography?(tandem) mass spectrometry

Simple and sensitive liquid chromatography/tandem mass spectrometry method for the determination of diazepam and its major metabolites in rat cerebrospinal fluid

An Overview on Total Analytical Methods for the Detection of 1,4-Benzodiazepines

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