Liquid Chromatography

Lembke, Peter; Henze, Günter; Cabrera, Karin; Brünner, Wolfgang; Müller, Egbert

doi:10.1002/14356007.b05_237

Cited by 1 publication

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“…Detection by diode-array or more generally by UV is very suitable for the multiresidue method ,, . However, due to the low sensitivity of the UV detectors and the presence of UV interferents in extracts, LC−MS techniques have been widely applied for the determination of a wide range of residual pesticides ,− . Anyway, as the number of pesticides in coated seeds is generally between one and three and their content is very high, the use of UV detection is sufficient for the quantification of pesticides on seed coatings.…”

Section: Introductionmentioning

confidence: 99%

Development of a Rapid Determination of Pesticides in Coated Seeds Using a High-Performance Liquid Chromatography−UV Detection System

Bourgin¹,

Bize²,

Durand³

et al. 2009

J. Agric. Food Chem.

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For the determination of pesticides in coated seeds, this study compared two HPLC-UV methods, using a short column or a conventional column, as well as two extraction procedures, by ultrasonic extraction at room temperature or by pressurized liquid extraction (PLE). The comparison of selected column performances showed that the short column enabled the 3-fold reduction of analysis time (ca. 9 min vs 29 min) and eluent consumption (ca. 6.1 mL vs 20.8 mL) for the separation of five insecticides (bitertanol, fludioxonil, imidacloprid, metalaxyl-M and tefluthrin) and one bird repellent (anthraquinone) without altering peak resolutions. Recovery rates for pressurized liquid extraction at 120 degrees C were similar (between 84% and 102%) to those obtained by ultrasonication. Both methods were then applied for the extraction of loaded seeds. Rates for ultrasonic extraction at room temperature were lower (from 16% to 95%) than those observed for recovery tests, unlike PLE at 120 degrees C which showed good rates, ranging between 82% and 95%, for all the loaded pesticides.

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