Liquid Chromatography coupled to Isotope Ratio Mass Spectrometry (LC-IRMS): A review

Perini, Matteo; Bontempo, Luana

doi:10.1016/j.trac.2021.116515

Cited by 26 publications

(9 citation statements)

References 82 publications

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“…The development of LC-IRMS application methods is challenging due to the content of freshly stewed bird's nest components, the need for a mobile phase free of organic solvents and certain limitations of the system. 28 Therefore, the requirements for the columns are more stringent. As sialic acid is an “acidic sugar”, two columns were chosen for comparison that retains polar substances well and is resistant to pure aqueous solutions: the Hyper REZ XP Carbohydrate H+ column (300 mm × 7.7 mm, 8 μm) and the Syncronis aQ (250 mm × 4.6 mm, 5 μm) liquid chromatographic columns.…”

Section: Resultsmentioning

confidence: 99%

Stable isotope ratio analysis of carbon to distinguish sialic acid from freshly stewed bird's nest products

Feng

Wang

et al. 2022

Anal. Methods

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Section: Resultsmentioning

confidence: 99%

Stable isotope ratio analysis of carbon to distinguish sialic acid from freshly stewed bird's nest products

Feng

Wang

et al. 2022

Anal. Methods

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“…The technology potentially has broad use and improved detection of a variety of substances that are applicable to academic and industrial research as reviewed in the literature. 20,21 Here we apply this technology as an interface between LC and IRMS to measure the CO 2 and forego the methanizer to the FID in order to expand the technique to IRMS sourcing applications. We establish a proof-of-principle with flow injection analysis of carbon containing analytes dissolved in H 2 O−CH 3 OH solvent, reporting precision, trueness, and linearity of repeated δ 13 C VPDB measurements.…”

Section: T H Imentioning

confidence: 99%

“…This is a new carbon selective detector system recently made commercially available in 2021 for the conversion of LC analytes to CO 2 and then methanized to CH 4 to enable quantitative flame ionization detector (FID) measurement with LC separations demonstrated using various mobile phases and reported through application notes on the ARC Web site. The technology potentially has broad use and improved detection of a variety of substances that are applicable to academic and industrial research as reviewed in the literature. , Here we apply this technology as an interface between LC and IRMS to measure the CO 2 and forego the methanizer to the FID in order to expand the technique to IRMS sourcing applications. We establish a proof-of-principle with flow injection analysis of carbon containing analytes dissolved in H 2 O–CH 3 OH solvent, reporting precision, trueness, and linearity of repeated δ 13 C VPDB measurements.…”

Section: Introductionmentioning

confidence: 99%

Liquid Chromatography Coupled to Isotope Ratio Mass Spectrometry Expanded to Include Organic Mobile Phases

Tobias,

Jones,

Saunders

et al. 2024

Anal. Chem.

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Current commercially available liquid chromatography coupled to isotope ratio mass spectrometry systems (LC-IRMS) oxidize all eluent and thus can only operate with all-aqueous mobile phases, limiting their application to a small subset of analytes and mixtures that can be separated without organic solvents. We report a novel rotating-catalytic disc desolvation device with subsequent laser-activated photocatalytic analyte combustion to create CO 2 for high precision carbon isotope ratio measurements compatible with both aqueous and organic liquid mobile phases. Sucrose, glucose, androsterone, or androsterone acetate in 20% and 50% H 2 O− CH 3 OH solutions were introduced by flow injection to the interface to IRMS for sugars and steroids, respectively. Sucrose δ 13 C VPDB linearity was excellent over 1−10 μg (33−655 nmol C) injections, using IRMS compatible He/1%O 2 oxidation gas. The limit of precise isotope analysis (LOIA) of δ 13 C VPDB was 1 μg (35 nmol C) for sucrose and 10 μg (655 nmol C) for androsterone with average precisions of SD(δ 13 C) ± 0.8‰. Calibration was performed with and bracketed the δ 13 C VPDB isotope ratio range using androsterone-acetate and glucose. With further development to improve sensitivity and application to chromatography, the prototype proof-of-principle LC-IRMS shows promise to resolve a major drawback in current LC-IRMS systems and may open LC-IRMS to many more compounds than currently possible.

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“…Advances in the field have been illustrated by analyzing from time-to-time both successful and unsuccessful current applications of the technique and by exploring the growing trend of incorporating isotopes with multiple markers. While the Molecules 2023, 28, 4300 3 of 26 present review does not include a comprehensive explanation of the current instrumentations and methodologies used, interested readers can find more information by referring to other extensive literature reviews [20][21][22][23][24][25][26].…”

Section: Introductionmentioning

confidence: 99%

Isotope Fingerprinting as a Backup for Modern Safety and Traceability Systems in the Animal-Derived Food Chain

et al. 2023

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In recent years, due to the globalization of food trade and certified agro-food products, the authenticity and traceability of food have received increasing attention. As a result, opportunities for fraudulent practices arise, highlighting the need to protect consumers from economic and health damages. In this regard, specific analytical techniques have been optimized and implemented to support the integrity of the food chain, such as those targeting different isotopes and their ratios. This review article explores the scientific progress of the last decade in the study of the isotopic identity card of food of animal origin, provides the reader with an overview of its application, and focuses on whether the combination of isotopes with other markers increases confidence and robustness in food authenticity testing. To this purpose, a total of 135 studies analyzing fish and seafood, meat, eggs, milk, and dairy products, and aiming to examine the relation between isotopic ratios and the geographical provenance, feeding regime, production method, and seasonality were reviewed. Current trends and major research achievements in the field were discussed and commented on in detail, pointing out advantages and drawbacks typically associated with this analytical approach and arguing future improvements and changes that need to be made to recognize it as a standard and validated method for fraud mitigation and safety control in the sector of food of animal origin.

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Liquid Chromatography coupled to Isotope Ratio Mass Spectrometry (LC-IRMS): A review

Cited by 26 publications

References 82 publications

Stable isotope ratio analysis of carbon to distinguish sialic acid from freshly stewed bird's nest products

Stable isotope ratio analysis of carbon to distinguish sialic acid from freshly stewed bird's nest products

Liquid Chromatography Coupled to Isotope Ratio Mass Spectrometry Expanded to Include Organic Mobile Phases

Isotope Fingerprinting as a Backup for Modern Safety and Traceability Systems in the Animal-Derived Food Chain

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