Liquid chromatography–electrospray tandem mass spectrometric method for quantification of monensin in plasma and edible tissues of chicken used in pharmacokinetic studies: Applying a total error approach

Chéneau, Estelle; Henri, Jérôme; Pirotais, Yvette; Abjean, Jean-Pierre; Roudaut, Brigitte; Sandérs, Pascal; Laurentie, Michel

doi:10.1016/j.jchromb.2006.10.072

Cited by 30 publications

(23 citation statements)

References 21 publications

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“…Edinburgh, UK). 3,11,12 Lower concentrations may have been found in earlier studies had methods with lower detection limits been used. In the present case series report, heart monensin concentrations were consistently higher than those detected in the liver.…”

Section: Research-article2014mentioning

confidence: 95%

Diagnostic value of tissue monensin concentrations in horses following toxicosis

et al. 2014

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Two separate incidents of monensin exposure in horses resulting in toxicosis provided insight into the diagnostic value and interpretive criteria of various biological samples. In case 1, 25 horses broke into a shed and ingested feed that was supplemented with 800 g/ton (880 µg/g) of monensin. Within 48 hr, 1 horse had died, 2 developed cardiac arrhythmias, lethargy, and recumbency, and another was euthanized due to severe deterioration. Minimal histologic lesions were noted in the horse that died peracutely, while another showed characteristic lesions of acute cardiomyocyte degeneration and necrosis. Stomach content, heart, liver, urine, and serum revealed various detectable concentrations of monensin in clinically affected and unaffected horses with known exposure. In case 2, a pastured horse had access to a mineral mix containing 1,600 g/ton (1,760 µg/g) of monensin. Within 48 hr, the horse became symptomatic and was euthanized because of severe respiratory distress. Histologic cardiac lesions were minimal but detectable amounts of monensin were found in blood, heart, liver, and stomach contents. In both cases, monensin toxicosis was confirmed with toxicological analysis. These cases demonstrate an overall lack of correlation of monensin concentrations in various biological samples with clinical outcome. However, serum, urine, blood, liver, heart, and stomach content can be tested to confirm exposure. More importantly, the consistently higher concentrations found in heart tissue suggest this is the most useful diagnostic specimen for postmortem confirmation of toxicosis in horses especially in cases in which associated feed cannot be tested for monensin or in cases with no histologic lesions.

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Section: Research-article2014mentioning

confidence: 95%

Diagnostic value of tissue monensin concentrations in horses following toxicosis

et al. 2014

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“…While the latter group can be characterized with similar structural elements, the group of chemical coccidiostats is rather heterogeneous. This might be the reason why many methods target only a limited number of coccidiostats [4][5][6][7][8][9][10][11][12][13][14] or only the group of ionophores [15][16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Determination of Coccidiostats in Milk Products by LC–MS and Its Application to a Fermentation Experiment

et al. 2012

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A liquid chromatographic tandem mass spectrometric (LC-MS-MS) method for the determination of five chemical coccidiostats (decoquinate, diclazuril, halofuginone, nicarbazin, and robenidine) and five ionophore coccidiostats (maduramicin, monensin, narasin, salinomycin, and semduramicin) in yoghurt, kefir, and sour cream is presented. Lasalocid, the sixth ionophore listed in 124/2009/EC was not included because of its extremely dissimilar behavior during sample preparation. Main steps of the method include extraction with acetonitrile, centrifugation, clean-up on Oasis HLB solid phase extraction cartridge, evaporation under nitrogen stream, and LC-MS-MS determination. Selectivity, linearity, sensitivity, accuracy, repeatability, within-laboratory reproducibility, limit of determination, and limit of quantitation were determined during the validation procedure. The method proved to be applicable for both qualitative and quantitative determination of the ten above-mentioned target compounds. In our in-house fermentation experiments, milk fortified with coccidiostats was fermented to get yoghurt, kefir, and sour cream. Our results show that the coccidiostat content did not change significantly during fermentation for any of the target compounds.

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“…This approach has been used extensively for physicochemical methods [16] and Findlay et al [17] recommended using it for immunoassay validation in the pharmaceutical field. We suggest investigating the usefulness with an immunoassay method for residues of veterinary drugs.…”

Section: Introductionmentioning

confidence: 99%

Use of the total error approach to evaluate the performance of a semi-quantitative immunological method (BIACORE method) for detecting sulfamethazine in bovine milk

Laurentie

Gaudin

2009

Journal of Chromatography B

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Liquid chromatography–electrospray tandem mass spectrometric method for quantification of monensin in plasma and edible tissues of chicken used in pharmacokinetic studies: Applying a total error approach

Cited by 30 publications

References 21 publications

Diagnostic value of tissue monensin concentrations in horses following toxicosis

Diagnostic value of tissue monensin concentrations in horses following toxicosis

Determination of Coccidiostats in Milk Products by LC–MS and Its Application to a Fermentation Experiment

Use of the total error approach to evaluate the performance of a semi-quantitative immunological method (BIACORE method) for detecting sulfamethazine in bovine milk

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