Liquid chromatography-mass spectrometry for the determination of chemical contaminants in food

Hird, Simon; Lau, Benjamin P.‐Y.; Schuhmacher, Rainer; Krska, Rudolf

doi:10.1016/j.trac.2014.04.005

Cited by 166 publications

(90 citation statements)

References 140 publications

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“…The upper phase was removed and transferred to a clean 50 mL Falcon tube. Extraction salts were added: 1.5 g of CH 3 COONa and 6 g of MgSO 4 . The contents were mixed in a Vortex stirrer (2.5 min), centrifuged (6000 rpm, 10 min), and transferred to a clean Falcon tube.…”

Section: Sample Preparation Methods Developmentmentioning

confidence: 99%

“…The next stage was dispersive solid-phase extraction (dSPE). The following reagents were added: sorbents-400 mg of PSA and 400 mg of C 18 , and salts-1200 mg of MgSO 4 . The contents were mixed in a Vortex stirrer (2.5 min), centrifuged (6000 rpm, 10 min), and transferred to a glass tube.…”

Section: Sample Preparation Methods Developmentmentioning

confidence: 99%

“…This group of compounds includes zearalenone/fusarium-2 (ZEA/F-2) which occurs mainly in corn cobs, rice, cereals, and wheat [1][2][3][4]. Zearalenone is absorbed from contaminated food shortly after ingestion, and it is rapidly metabolized in the gastrointestinal tract to produce more toxic compounds: α-zearalanol (α-ZAL), α-zearalenol (α-ZEL), β-zearalanol (β-ZAL), and β-zearalenol (β-ZEL).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The determination of zearalenone and its major metabolites in endometrial cancer tissues

et al. 2018

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Endometrial cancer is one of the most commonly diagnosed cancers in women. The search for factors that contribute to the development of cancer cells in reproductive organs should involve the detection of xenoestrogens, in particular zearalenone (ZEA) and its metabolites. Xenoestrogens are endocrine disruptors-ZEA and its metabolites are structurally similar to estrogens (macrocyclic lactone ring) and show high affinity for estrogen receptors. This study proposes a new method for the preparation of samples of human tissues with endometrial cancer by the use of the QuEChERS technique. Analytical parameters such as centrifugation temperature, extraction solvent, and adsorbents were modified to obtain satisfactory recovery for ZEA (R = 82.6%, RSD = 2.9%) and one of its metabolites, α-zearalenol (R = 50.1%, RSD = 3.2%). High-performance liquid chromatography (HPLC) with fluorescence detection and tandem mass spectrometry (LC-QTOF-MS) were used for the identification and quantitative determination of the analyzed compounds. The developed procedure was applied for analyses of human tissues with endometrial cancer. The presence of α-zearalenol was detected in 47 out of the 61 examined tissue samples.

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Section: Sample Preparation Methods Developmentmentioning

confidence: 99%

Section: Sample Preparation Methods Developmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The determination of zearalenone and its major metabolites in endometrial cancer tissues

et al. 2018

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“…A detailed overview and discussion of the advantages and disadvantages of current MS systems allowing measurements in low, medium and (ultra)high mass resolving power modes was provided in several recent comprehensive reviews [18,19]. From the available studies, the MS detectors used in mycotoxin analysis include triple quadrupole (QqQ), ion trap, time-of-flight (TOF), and orbital ion trap mass analyzers, as well as hybrid systems that combine two types of analyzers.…”

Section: Introductionmentioning

confidence: 99%

Advanced LC–MS-based methods to study the co-occurrence and metabolization of multiple mycotoxins in cereals and cereal-based food

Malachová

Stranska-Zachariasova

Václavíková

et al. 2017

Anal Bioanal Chem

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130

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Liquid chromatography (LC) coupled with mass spectrometry (MS) is widely used for the determination of mycotoxins in cereals and cereal-based products. In addition to the regulated mycotoxins, for which official control is required, LC-MS is often used for the screening of a large range of mycotoxins and/or for the identification and characterization of novel metabolites. This review provides insight into the LC-MS methods used for the determination of co-occurring mycotoxins with special emphasis on multiple-analyte applications. The first part of the review is focused on targeted LC-MS approaches using cleanup methods such as solid-phase extraction and immunoaffinity chromatography, as well as on methods based on minimum cleanup (quick, easy, cheap, effective, rugged, and safe; QuEChERS) and dilute and shoot. The second part of the review deals with the untargeted determination of mycotoxins by LC coupled with high-resolution MS, which includes also metabolomics techniques to study the fate of mycotoxins in plants.

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“…The gold standard for confirmatory analysis is tandem mass spectrometry (MS/MS) using triple-quadrupole (QqQ) or (linear) ion trap [(L)IT] mass spectrometry operated in selected reaction monitoring (SRM), isolating one precursor ion with a 1 Dam/z selection window and monitoring two selected product ions [9]. Using this set-up, four identification points are rewarded according to EU regulation [1], which is sufficient for the analysis of both regulated and banned compounds [1,2].…”

Section: Introductionmentioning

confidence: 99%

The Assessment of Selectivity in Different Quadrupole-Orbitrap Mass Spectrometry Acquisition Modes

Berendsen

Wegh

Meijer

et al. 2014

J. Am. Soc. Mass Spectrom.

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Abstract. Selectivity of the confirmation of identity in liquid chromatography (tandem) mass spectrometry using Q-Orbitrap instrumentation was assessed using different acquisition modes based on a representative experimental data set constructed from 108 samples, including six different matrix extracts and containing over 100 analytes each. Single stage full scan, all ion fragmentation, and product ion scanning were applied. By generating reconstructed ion chromatograms using unit mass window in targeted MS 2 , selected reaction monitoring (SRM), regularly applied using triplequadrupole instruments, was mimicked. This facilitated the comparison of single stage full scan, all ion fragmentation, (mimicked) SRM, and product ion scanning applying a mass window down to 1 ppm. Single factor Analysis of Variance was carried out on the variance (s 2 ) of the mass error to determine which factors and interactions are significant parameters with respect to selectivity. We conclude that selectivity is related to the target compound (mainly the mass defect), the matrix, sample clean-up, concentration, and mass resolution. Selectivity of the different instrumental configurations was quantified by counting the number of interfering peaks observed in the chromatograms. We conclude that precursor ion selection significantly contributes to selectivity: monitoring of a single product ion at high mass accuracy with a 1 Da precursor ion window proved to be equally selective or better to monitoring two transition products in mimicked SRM. In contrast, monitoring a single fragment in all ion fragmentation mode results in significantly lower selectivity versus mimicked SRM. After a thorough interlaboratory evaluation study, the results of this study can be used for a critical reassessment of the current identification points system and contribute to the next generation of evidence-based and robust performance criteria in residue analysis and sports doping.

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Liquid chromatography-mass spectrometry for the determination of chemical contaminants in food

Cited by 166 publications

References 140 publications

The determination of zearalenone and its major metabolites in endometrial cancer tissues

The determination of zearalenone and its major metabolites in endometrial cancer tissues

Advanced LC–MS-based methods to study the co-occurrence and metabolization of multiple mycotoxins in cereals and cereal-based food

The Assessment of Selectivity in Different Quadrupole-Orbitrap Mass Spectrometry Acquisition Modes

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