Liquid Chromatography-Mass Spectrometry (LC-MS)-Based Analysis of Molecular Lipids in Algae Samples

Nygrén, Heli; Seppänen‐Laakso, Tuulikki; Rischer, Heiko

doi:10.1007/7651_2017_108

Cited by 5 publications

(3 citation statements)

References 9 publications

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“…Lipid extraction-Lipid extraction was performed based on a previously published protocol 20,21 . Briefly, serum samples were thawed on ice at room temperature (30 min).…”

Section: Metabolite Extraction By Meoh-h 2 O-mentioning

confidence: 99%

Metabolic Profiling and Compound-Class Identification Reveal Alterations in Serum Triglyceride Levels in Mice Immunized with Human Vaccine Adjuvant Alum

et al. 2019

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Alum has been widely used as an adjuvant for human vaccines; however, the impact of Alum on host metabolism remains largely unknown. Herein, we applied mass spectrometry (LCMS)-based metabolic and lipid profiling to monitor the effects of Alum adjuvant on mouse serum at 6, 24, 72 and 168 h post-vaccination. We propose a new strategy termed Subclass Identification and Annotation for Metabolomics (SIAM) for class-wise identification of untargeted metabolomics data generated from high-resolution MS. Using this approach, we identified and validated the levels of several lipids in mouse serum that were significantly altered following Alum administration. These lipids showed a biphasic response even 168 h after vaccination. The majority of the lipids were triglycerides (TAGs), where TAGs with long chain unsaturated fatty acids were decreased at 24 h, and TAGs with short chain fatty acids were decreased at 168 h. To *

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“…Lipid extraction-Lipid extraction was performed based on a previously published protocol 20,21 . Briefly, serum samples were thawed on ice at room temperature (30 min).…”

Section: Metabolite Extraction By Meoh-h 2 O-mentioning

confidence: 99%

Metabolic Profiling and Compound-Class Identification Reveal Alterations in Serum Triglyceride Levels in Mice Immunized with Human Vaccine Adjuvant Alum

et al. 2019

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“…Lipids can also be analyzed using other chromatography techniques such as TLC or HPTLC [11,12] (Thin Layer Chromatography) or HPLC [13] (High Performance Liquid J o u r n a l P r e -p r o o f 6 Chromatography). These chromatographic techniques can be hyphenated with MS (Mass Spectrometry) detection [14][15][16]. This is a non-exhaustive list for the analysis of the extracted lipids, but these offline techniques all require considerable amount of time.…”

Section: Introductionmentioning

confidence: 99%

Using benchtop NMR spectroscopy as an online non-invasive in vivo lipid sensor for microalgae cultivated in photobioreactors

Bouillaud

Drouin

Charrier

et al. 2020

Process Biochemistry

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HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

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“…That is, we are able to pinpoint whether thermal stress affects thylakoid membranes by characterizing their specific glycolipids rather than bulk fatty acids derived from other pool of lipids such as the triglycerides or phospholipids. Such detailed and comprehensive lipid analysis could only be achieved by recent analytical developments in mass spectrometry and the advance of lipidomics (Jones et al, 2012;Yao et al, 2015;Nygren et al, 2017).…”

Section: Lipid Membrane Profiles Of Symbionts and Their Fate After Thmentioning

confidence: 99%

The role of >i<Symbiodinium>/i< membrane lipids in response to heat shock: implications for coral bleaching

Botana¹

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Coral reefs around the world have been largely devastated by the phenomenon of "coral bleaching", which causes have been reported to be strongly related to oxidative stress promoted by climate change drivers, including mainly global warming and catastrophic El Niño events. Genetic variability in coral endosymbionts from the Family Symbiodiniacea was also suggested as determinant of host susceptibility to stress because they present distinct physiological boundaries when in free living or in symbiosis. Here we present for the first time the successful use of lipidomics (i.e., the global characterization of lipids in a given organism) supporting molecular investigation in the oxidative mechanisms related to thermal stress in coral endosymbionts phylotypes. Symbiodinium minutum was thermal sensitive, whereas S. microadriaticum and S. goreaui presented different levels of thermal tolerance. Their lipid phenotypes after stress, including the photosystem electron transporter-plastoquinone-suggested they had different survival strategies. In addition, chloroplast specific lipids with polyunsaturated fatty acids (PUFAs) mainly formed by omega 3 (n-3) seemed to be essential to sustain Symbiodinium cells bioenergetics in the long term (10 days after stress). S. microadriaticum and S. goreaui capability of keeping high n-3 concentrations in the chloroplast membranes determined their survival. The present thesis reports, for the first-time, upregulation of oxidized lipids derived from precursor chloroplast membranes and free fatty acids (FFA) in response to oxidative stress damage caused by heat. The study of lipid membranes is of paramount importance to better understand the bioenergetics of symbionts and to determine the host/endosymbiont vulnerability to climate change stressors in a warmer future.

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Liquid Chromatography-Mass Spectrometry (LC-MS)-Based Analysis of Molecular Lipids in Algae Samples

Cited by 5 publications

References 9 publications

Metabolic Profiling and Compound-Class Identification Reveal Alterations in Serum Triglyceride Levels in Mice Immunized with Human Vaccine Adjuvant Alum

Metabolic Profiling and Compound-Class Identification Reveal Alterations in Serum Triglyceride Levels in Mice Immunized with Human Vaccine Adjuvant Alum

Using benchtop NMR spectroscopy as an online non-invasive in vivo lipid sensor for microalgae cultivated in photobioreactors

The role of >i<Symbiodinium>/i< membrane lipids in response to heat shock: implications for coral bleaching

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