Liquid chromatography quadrupole linear ion trap mass spectrometry for quantitative steroid hormone analysis in plasma, urine, saliva and hair

Gaudl, Alexander; Kratzsch, Juergen; Bae, Yoon Ju; Kieß, Wieland; Thiery, Joachim; Ceglarek, Uta

doi:10.1016/j.chroma.2016.07.087

Cited by 123 publications

(72 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Finally, we used saliva samples to measure the neurosteroid levels in this study. Although a previous study demonstrated that hormone concentrations in plasma, urine, saliva, and hair are highly correlated72, the DHEA(S) levels measured in saliva may not necessarily represent the concentration and activity of neurosteroids in the brain.…”

Section: Discussionmentioning

confidence: 94%

Polymorphisms of STS gene and SULT2A1 gene and neurosteroid levels in Han Chinese boys with attention-deficit/hyperactivity disorder: an exploratory investigation

Wang

Chan

Chou

et al. 2017

Sci Rep

View full text Add to dashboard Cite

This study examined the relationships among polymorphisms of the STS gene and SULT2A1 gene, dehydroepiandrosterone (DHEA) and its sulfated form (DHEA-S), and characteristics of attention-deficit/hyperactivity disorder (ADHD). We used cheek swabs to obtain the genomic DNA of 200 ADHD male probands (mean age: 8.7 years), 192 patients’ mothers and 157 patients’ fathers. Three SNPs in the STS gene (rs6639786, rs2270112, and rs17268988) and one SNP in the SULT2A1 gene (rs182420) were genotyped. Saliva samples were collected from the ADHD patients to analyze DHEA and DHEA-S levels. The behavioral symptoms were evaluated with the Swanson, Nolan, and Pelham, and Version IV Scale for ADHD (SNAP-IV), and the neuropsychological function was assessed using the Conners’ Continuous Performance Tests (CPT). We found the C allele of rs2270112 within the STS gene to be over-transmitted in males with ADHD. Polymorphisms of rs182420 within the SULT2A1 gene were not associated with ADHD. In addition, the C allele carriers of rs2270112 demonstrated significantly higher DHEA-S levels than the G allele carriers. Levels of DHEA were positively correlated with attention as measured by the CPT. These findings support a potential role in the underlying biological pathogenesis of ADHD with regard to STS polymorphisms and neurosteroid levels.

show abstract

Section: Discussionmentioning

confidence: 94%

Polymorphisms of STS gene and SULT2A1 gene and neurosteroid levels in Han Chinese boys with attention-deficit/hyperactivity disorder: an exploratory investigation

Wang

Chan

Chou

et al. 2017

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Last but not least, an undesirable effect of pulverisation was mentioned by Gaudl et al . where homogenization of hair samples with a ball mill resulted in worsened reproducibility and there was no benefit in signal/noise ratios 23 . Worth pointing out is that in our extraction protocol cooling of the samples in liquid nitrogen prevents a negative effect of heat during the homogenization and it ameliorates the pulverisation by making the hairs more brittle.…”

Section: Discussionmentioning

confidence: 99%

Testosterone-like immunoreactivity in hair measured in minute sample amounts - a competitive radioimmunoassay with an adequate limit of detection

Slezak

Ström

Theodorsson

2017

Sci Rep

View full text Add to dashboard Cite

The concentrations of testosterone deposited in hair during hair growth may provide a retrospective reflection of the concentrations of bioactive testosterone in plasma. The objective of this study was to develop a radioimmunoassay with a sufficiently low limit of detection to measure the testosterone-like immunoreactivity in smaller hair samples (5 mg) than used in earlier studies, and to compare three different extraction procedures. The competitive radioimmunoassay consisted of a polyclonal antiserum (immunogen testosterone-7α-BSA) and a radioligand synthesised from testosterone-3-CMO-histamine. The within-assay and total coefficients of variation in the working range was 3% and 4.5%, respectively. The limit of detection was 0.87 pg/mL, which is equivalent to 0.12 pg/mg testosterone in 5 mg of hair. The concentration of testosterone-like immunoreactivity in hair samples was 1.23 (SD 0.47) pg/mg in women and 2.67 (SD 0.58) pg/mg in men (pulverised hair). Significantly improved precision was found when pulverised hair was used compared to non-pulverised hair. Our data indicate that pulverisation of the hair prior to hormone extraction is crucial. Detection limits fit for the intended purpose are achievable with 5 mg samples of hair.

show abstract

“…Several new LC-MS/MS-based methods for multiple detections of both androgenic and estrogenic steroids have recently been developed (4,14,54). However, simultaneous measurement of multiple steroids often comes at the cost of decreased assay sensitivity.…”

Section: Methodological Aspectsmentioning

confidence: 99%

Sex-specific estrogen levels and reference intervals from infancy to late adulthood determined by LCMS/MS

Frederiksen¹,

Th²,

Andersen³

et al. 2020

ESPE

View full text Add to dashboard Cite

The lack of sensitive and robust analytical methods has hindered the reliable quantification of estrogen metabolites in subjects with low concentrations. Objective: To establish sex-specific reference ranges for estrone (E1) and estradiol (E2) throughout life and to evaluate sex-differences using the state-of-the-art liquid chromatography tandem mass spectrometry (LC-MS/MS) method for quantification of E1, E2, and estriol (E3). Design: LC-MS/MS method development and construction of estrogen reference ranges. Settings: Population-based cross-sectional cohorts from the greater Copenhagen and Aarhus areas. Participants: Healthy participants aged 3 months to 61 years (n = 1838). Results: An isotope diluted LC-MS/MS method was developed and validated for measurements of serum E1, E2, and E3. Limits of detections (LODs) were 3 pmol/L (E1), 4 pmol/L (E2), and 12 pmol/L (E3), respectively. This sensitive method made it possible to differentiate between male and female concentration levels of E1 and E2 in children. In girls, E2 levels ranged from

show abstract

Liquid chromatography quadrupole linear ion trap mass spectrometry for quantitative steroid hormone analysis in plasma, urine, saliva and hair

Cited by 123 publications

References 26 publications

Polymorphisms of STS gene and SULT2A1 gene and neurosteroid levels in Han Chinese boys with attention-deficit/hyperactivity disorder: an exploratory investigation

Polymorphisms of STS gene and SULT2A1 gene and neurosteroid levels in Han Chinese boys with attention-deficit/hyperactivity disorder: an exploratory investigation

Testosterone-like immunoreactivity in hair measured in minute sample amounts - a competitive radioimmunoassay with an adequate limit of detection

Sex-specific estrogen levels and reference intervals from infancy to late adulthood determined by LCMS/MS

Contact Info

Product

Resources

About

Liquid chromatography quadrupole linear ion trap mass spectrometry for quantitative steroid hormone analysis in plasma, urine, saliva and hair

Cited by 123 publications

References 26 publications

Polymorphisms of STS gene and SULT2A1 gene and neurosteroid levels in Han Chinese boys with attention-deficit/hyperactivity disorder: an exploratory investigation

Polymorphisms of STS gene and SULT2A1 gene and neurosteroid levels in Han Chinese boys with attention-deficit/hyperactivity disorder: an exploratory investigation

Testosterone-like immunoreactivity in hair measured in minute sample amounts - a competitive radioimmunoassay with an adequate limit of detection

Sex-specific estrogen levels and reference intervals from infancy to late adulthood determined by LCMS/MS

Contact Info

Product

Resources

About

Sex-specific estrogen levels and reference intervals from infancy to late adulthood determined by LCMS/MS