Liquid chromatography–tandem mass spectrometry for the simultaneous quantitation of artemether and lumefantrine in human plasma: Application for a pharmacokinetic study

César, Isabela Costa; Ribeiro, José Antônio de Aquino; Teixeira, Leonardo de Souza; Bellorio, Karini Bruno; Abreu, Fernanda Crunivel de; Moreira, Josianny Mesquita; Chellini, Paula Rocha; Pianetti, Gérson Antônio

doi:10.1016/j.jpba.2010.07.027

Cited by 33 publications

(19 citation statements)

References 23 publications

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“…The developed method uses 20 l of mouse WB or plasma which is the smallest sample volume as compared to other published methods [10][11][12][13][14][15]. The protein precipitation extraction method was robust, efficient and provided high recovery rates for LF in both WB and plasma.…”

Section: Intra-assay (N = 6)mentioning

confidence: 99%

“…The mean percentage recovery for LF in plasma, evaluated at high, medium and low concentrations, were 114.2% (%CV = 0.6), 109.8% (%CV = 2.7) and 100.5% (%CV = 8.7), respectively. Cesar et al [13] used a protein precipitation extraction method and reported recovery values for LF ranging from 81.4 to 83.2%. Munjal et al [12] also used a protein precipitation extraction method and reported a mean LF recovery of 58.8%.…”

Section: Intra-assay (N = 6)mentioning

confidence: 99%

“…), ensures that the method is robust and reproducible for analysing samples of unknown concentrations. There are five published LC-MS/MS methods for the quantification of LF in human plasma and one for the quantification of LF in rat plasma [10][11][12][13][14][15]. There seems to be no LC-MS/MS methods for the quantification of LF in mouse WB or plasma.…”

Section: Introductionmentioning

confidence: 99%

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Development and validation of a LC–MS/MS method for the quantitation of lumefantrine in mouse whole blood and plasma

Govender

Gibhard

Plessis

et al. 2015

Journal of Chromatography B

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Section: Intra-assay (N = 6)mentioning

confidence: 99%

Section: Intra-assay (N = 6)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Development and validation of a LC–MS/MS method for the quantitation of lumefantrine in mouse whole blood and plasma

Govender

Gibhard

Plessis

et al. 2015

Journal of Chromatography B

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“…Some other recent methods for the assay of these drugs alone or in combination with other antimalarials have also been reported [24][25][26][27][28]. However, these methods still had some limitations related to the chromatography and detection in terms of resolution, time-consuming, and requiring prolonged derivatization.…”

Section: Introductionmentioning

confidence: 99%

Highly sensitive liquid chromatographic analysis of artemisinin and its derivatives after pre-column derivatization with 4-carboxyl-2,6-dinitrobenzene diazonium ion

Adegoke¹,

Xiang²,

Chen³

2012

Acta Chromatographica

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Summary.A highly sensitive and reproducible isocratic liquid chromatographic method has been developed for the analysis of artemisinin and its three commonly used derivatives (artesunate, dihydroartemisinin, and artemether). The method involves a precolumn derivatization reaction with 4-carboxyl-2,6-dinitrobenzene diazonium ion to produce azo adducts that are UV-active. The critical parameters for the derivatization such as temperature, reaction time, and reagent concentrations were studied and optimized. The chromatographic separations were carried out on a C-18 column with mobile phase consisting of acetonitrile-0.1% acetic acid (60:40) at a flow rate of 1 mL min −1 . UV detection was set at 254 nm. Dynamic linear calibration range was obtained at concentrations of artemisinins ranging from 0.26 to 1.44 μg mL −1 . The low limits of detections of artemisinin, artesunate, dihydroartemisinin, and artemether were found to be 0.091, 0.0125, 0.0489, and 0.0128 ng μL −1 , respectively. The developed methods were precise (RSD <3%) and accurate (% error <5%). The developed methods may find application in dosage form analysis and pharmacokinetic studies.

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“…Early attempts on application of LC–MS method for determination of LF in human plasma failed due to matrix effect (ME) [11]. Recently, several LC–MS/MS methods were reported, but the issue of ME has not been addressed sufficiently [12–15]. Using protein precipitation for sample preparation, Hodel et al reported the first LC–MS/MS method for LF, but the ME was significant: 22.7–29.1% (100% = no ME) [12], while César et al claimed the ME in their method was within the 85 to 115% range, but the calibration curve required a weighted quadratic regression [15].…”

mentioning

confidence: 99%

Determination of Lumefantrine in Small-Volume Human Plasma By Lc–MS/MS: Using A Deuterated Lumefantrine to Overcome Matrix Effect And Ionization Saturation

Huang

Marzan

et al. 2012

Bioanalysis

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Background To support pediatric study, a method to determine lumefantrine (LF) with small sample volume is needed. Matrix effect (ME) is a daunting issue in LF quantification in human plasma with LC MS/MS. Results Here we report an LC–MS/MS method with a deuterated LF as the internal standard (IS). Plasma sample (25 μl) was acidified with 5% formic acid prior to extraction with ethyl acetate. The recovery was over 80%. The absolute ME was within the range of 100 ± 8% for both LF and the IS, but cumulative ME was observed via large variation of IS as the IS. The linear signal. The cumulative ME and ionization saturation were overcome with the co-eluting LF-D9 range of calibration curve was 50–20,000 ng/ml. Conclusion ME and ionization saturation was overcome with a deuterated IS. The method utilized a small sample volume, suitable for pediatric study with capillary tube blood collection method.

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Liquid chromatography–tandem mass spectrometry for the simultaneous quantitation of artemether and lumefantrine in human plasma: Application for a pharmacokinetic study

Cited by 33 publications

References 23 publications

Development and validation of a LC–MS/MS method for the quantitation of lumefantrine in mouse whole blood and plasma

Development and validation of a LC–MS/MS method for the quantitation of lumefantrine in mouse whole blood and plasma

Highly sensitive liquid chromatographic analysis of artemisinin and its derivatives after pre-column derivatization with 4-carboxyl-2,6-dinitrobenzene diazonium ion

Determination of Lumefantrine in Small-Volume Human Plasma By Lc–MS/MS: Using A Deuterated Lumefantrine to Overcome Matrix Effect And Ionization Saturation

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