Liquid chromatography–tandem mass spectrometry measurement of 26 steroid hormones in human serum and plasma samples

Abstract

The measurement of steroid hormones provided critical information in the clinical evaluation of endocrine disorders. In this study, we developed a highthroughput solid-phase extraction method for the analysis of 26 steroids in human serum and plasma samples by liquid chromatography-tandem mass spectrometry. Chromatographic conditions and sample preparation were optimized to achieve good separation and maximum sensitivity for these analytes.Under the optimum conditions, good linearities were achieved in the quantitative range for each steroid hormone with the correlation coefficients (r) larger than 0.99. The limits of quantitation of the method were in the range from 0.0005 to 0.7901 ng/mL. The recoveries were in the range of 87.2-114.2% with intra-and interday precision lower than 9.94%. This method has already been applied to series of samples from clinical trials, and there was no significant difference between serum and ethylenediaminetetraacetic acid plasma samples.

Simultaneous quantitation of 17 female sex hormones in illegal products by validated liquid chromatography‐high resolution mass spectrometry and liquid chromatography‐tandem mass spectrometry methods

“…Therefore, we aimed to develop a method for the simultaneous measurement of 17 female sex hormones using LC-HRMS and LC-MS/MS. Among the 17 compounds analyzed, 11 of the hormone compounds had not been considered in the previous studies [17,18,33] and are being analyzed simultaneously for the first time in this study. In addition, this is the first study to evaluate female sex hormones in diverse sample types and dosage forms.…”