Liquid culture for efficient in vitro propagation of adventitious shoots in wild Vaccinium vitis-idaea ssp. minus (lingonberry) using temporary immersion and stationary bioreactors

Arigundam, Usha; Variyath, Asokan Mulayath; Siow, Yaw L.; Marshall, Dawn H; Debnath, Samir C.

doi:10.1016/j.scienta.2020.109199

Cited by 37 publications

(53 citation statements)

References 27 publications

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“…Regeneration is achieved through organogenesis through unipolar organ (shoots or roots) formation, as well as by somatic embryogenesis, where the somatic embryos' root and shoot meristem are developed [65]. Shoot organogenesis in lingonberries can be started with bud formation on explants, the elongation of the buds into shoots and the rooting of the shoots to form whole plants (Figure 3) [58]. Regeneration is a part of many micropropagation protocols; for example, haploid plants require adventitious shoot regeneration to form whole plants from somatic cells.…”

Section: Adventitious Shoot Regenerationmentioning

confidence: 99%

“…Ex vitro rooting is more common and successful for rooting lingonberry microshoots. While Miners et al [56] reported the ex vitro rooting of Red Pearl lingonberry microshoots in a humidity chamber (98% relative humidity) without any PGR treatment, Arigundam et al [58] treated leaf culture-derived wild lingonberry (ssp. minus) shoots with 39.4 mM of IBA powder, planted in two peats : one perlite (v/v) medium and maintained at 24 ± 2 °C and 95% humidity for a 16 h photoperiod (55 µ mol m −2 s −1 photosynthetic photon flux, PPF) for rooting.…”

Section: Rooting and Acclimatizationmentioning

confidence: 99%

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In Vitro Propagation Strategies of Medicinally Important Berry Crop, Lingonberry (Vaccinium vitis-idaea L.)

Debnath

Arigundam

2020

Agronomy

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Lingonberry (Vaccinium vitis-idaea L.) is a health-promoting small fruit crop rich in antioxidant metabolites that helps to reduce the incidence of degenerative diseases. Being heterozygous, lingonberries cannot preserve genetic characteristics through seed propagation. Conventional vegetative propagation, although it produces true-to-type plants, is not economically viable. In vitro propagation can multiply plants much faster than conventional methods. A liquid cultures system under a bioreactor micropropagation system is of significant importance to increase the multiplication rates of in vitro-produced shoots. Enhanced vegetative growth and variation in biochemical constituents are observed in micropropagated plants. Clonal fidelity, although it may be a serious problem for commercial micropropagation, can be verified efficiently by molecular markers. The current review provides detailed and updated information on lingonberry micropropagation along with conventional methods and their effects on morphological, molecular and biochemical characteristics in micropropagated plants, filling the gap in literature.

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Section: Adventitious Shoot Regenerationmentioning

confidence: 99%

Section: Rooting and Acclimatizationmentioning

confidence: 99%

In Vitro Propagation Strategies of Medicinally Important Berry Crop, Lingonberry (Vaccinium vitis-idaea L.)

Debnath

Arigundam

2020

Agronomy

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“…The addition of 6-BA, 6-furfuryladenine (6-KN), or Thidiazuron (TDZ) at 1–12 µM increased the rate of regeneration, number of shoots per K. parviflora rhizome bud, and number of roots per shoot. Cytokinins are important PGRs that can promote axillary shoot multiplication [ 29 , 31 , 40 ], somatic embryogenesis [ 41 ], and adventitious shoot regeneration [ 42 ] in numerous plants. However, the application of cytokinin often adversely affects in vitro rhizogenesis [ 31 ].…”

Section: Resultsmentioning

confidence: 99%

Establishment of a Rapid Micropropagation System for Kaempferia parviflora Wall. Ex Baker: Phytochemical Analysis of Leaf Extracts and Evaluation of Biological Activities

Park

Kim

et al. 2021

Plants

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This study aimed to establish a rapid in vitro plant regeneration method from rhizome buds of Kaempferia parviflora to obtain the valuable secondary metabolites with antioxidant and enzyme inhibition properties. The disinfection effect of silver oxide nanoparticles (AgO NPs) on rhizome and effects of plant growth regulators on shoot multiplication and subsequent rooting were investigated. Surface sterilization of rhizome buds with sodium hypochlorite was insufficient to control contamination. However, immersing rhizome buds in 100 mg L−1 AgO NPs for 60 min eliminated contamination without affecting the survival of explants. The number of shoots (12.2) produced per rhizome bud was higher in Murashige and Skoog (MS) medium containing 8 µM of 6-Benzyladenine (6-BA) and 0.5 µM of Thidiazuron (TDZ) than other treatments. The highest number of roots (24), with a mean root length of 7.8 cm and the maximum shoot length (9.8 cm), were obtained on medium MS with 2 µM of Indole-3-butyric acid (IBA). A survival rate of 98% was attained when plantlets of K. parviflora were acclimatized in a growth room. Liquid chromatography with tandem mass spectrometry (LC-MS/MS) was used to determine the chemical profile of K. parviflora leaf extracts. Results showed that several biologically active flavonoids reported in rhizomes were also present in leaf tissues of both in vitro cultured and ex vitro (greenhouse-grown) plantlets of K. parviflora. We found 40 and 36 compounds in in vitro cultured and ex vitro grown leaf samples, respectively. Greenhouse leaves exhibited more potent antioxidant activities than leaves from in vitro cultures. A higher acetylcholinesterase inhibitory ability was obtained for greenhouse leaves (1.07 mg/mL). However, leaves from in vitro cultures exhibited stronger butyrylcholinesterase inhibitory abilities. These results suggest that leaves of K. parviflora, as major byproducts of black ginger cultivation, could be used as valuable alternative sources for extracting bioactive compounds.

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“…In vitrogrown shoots on a semi-solid medium and in a bioreactor containing liquid medium were used in this study. The morphological data of in vitro-grown propagated explants: micropropagated and conventionally propagated plants in greenhouse were collected 11 . Shoots vigor and plant vigor were determined by visual assessment, ranging from scale 1 (very poor) to 8 (fully normal and healthy plants with large green leaves and excellent vigor).…”

Section: Data Collectionmentioning

confidence: 99%

Epigenomic Insight of Lingonberry: Health Promoting Trait Under Micropropagation

Sikdar

Sharma

Barua

et al. 2021

Preprint

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Epigenetic variation plays a role in developmental gene regulation and responses to the environment. An efficient interaction of zeatin induced cytosine methylation and secondary compounds has been displayed for the first time in tissue-culture shoots of lingonberry (Vaccinium vitis-idaea) in vitro, in vivo and its cutting-cultivar Erntedank. Through MSAP assay, we observed highest methylated sites in leaf regenerants (LC1) from all primer combinations (108 bands), with their highest variation in secondary metabolites. We measured that four tissue-culture plants showed higher methylation bands than cutting propagated donor plants (ED) which exhibited 79 bands of methylation, which is comparatively low. On the other hand, we observed the highest total phenolic content in node culture-derived greenhouse grown plants, NC3 but leaf culture-derived greenhouse grown plants, LC1 represented low phenolic content. Our study showed more methylation in micropropagated plants (NC1, NC2, NC3, LC1) than those derived from cutting propagated ED plants, where methylation was not present. On the contrary, we observed higher secondary metabolites in ED plants but comparatively less in micropropagated shoots (NC1, NC2) and plants (NC3, LC1). Our study displayed that higher methylation sites observed in micropropagated plants possessed less amount of secondary metabolites.

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Liquid culture for efficient in vitro propagation of adventitious shoots in wild Vaccinium vitis-idaea ssp. minus (lingonberry) using temporary immersion and stationary bioreactors

Cited by 37 publications

References 27 publications

In Vitro Propagation Strategies of Medicinally Important Berry Crop, Lingonberry (Vaccinium vitis-idaea L.)

In Vitro Propagation Strategies of Medicinally Important Berry Crop, Lingonberry (Vaccinium vitis-idaea L.)

Establishment of a Rapid Micropropagation System for Kaempferia parviflora Wall. Ex Baker: Phytochemical Analysis of Leaf Extracts and Evaluation of Biological Activities

Epigenomic Insight of Lingonberry: Health Promoting Trait Under Micropropagation

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