Liquid-diet-induced alterations of rat parotid acinar cells studied by electron microscopy and enzyme cytochemistry

Hand, Arthur R.; Ho, Betty

doi:10.1016/0003-9969(81)90032-7

Cited by 52 publications

(32 citation statements)

References 37 publications

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“…Although early liquid diet studies reported atrophic acinar cells undergoing necrosis (Wilborn and Schneyer 1970) and degenerating acinar cells (Hand and Ho 1981), those studies were performed before the notion of apoptosis was widely accepted; therefore, several of the reported necrotic or degenerating acinar cells might have been apoptotic acinar cells. Taken together with other reports that acinar cell apoptosis occurs in parotid gland atrophy induced by different conditions (Walker and Gobe 1987;Chisholm et al 1995;Scott et al 1999), acinar cell apoptosis appears to be essential to atrophic changes of salivary glands, regardless of the cause of atrophy.…”

Section: Discussionmentioning

confidence: 99%

“…Our data strongly support this speculation, and suggest that depletion of acinar cells is a factor in decreased parotid gland weight. Previous studies that use histomorphometric analysis (Scott et al 1990;Scott and Gunn 1991) and TEM observations (Wilborn and Schneyer 1970;Hand and Ho 1981) have already shown that reduced parotid gland weight to be caused by the shrinkage of acinar cell; implying that liquid 12 diet-caused parotid gland atrophy results from both fewer and smaller acinar cells.…”

Section: Discussionmentioning

confidence: 99%

“…Parotid glands also reportedly show atrophic alterations in animals fed a liquid diet. In atrophic parotid glands induced by liquid diet, shrinkage of acinar cells, in which secretory granules decrease, has been morphologically observed (Hall and Schneyer 1964;Wilborn and Schneyer 1970;Hand and Ho 1981;Scott et al 1990;Scott and Gunn 1991) and reductions in parotid gland amylase activity have been shown by biochemical analysis (Hall and Schneyer 1964;Hand and Ho 1981;Johnson 1982;Scott and Gunn 1994).…”

Section: Introductionmentioning

confidence: 99%

“…

AbstractParotid glands of experimental animals fed a liquid diet are reported to show atrophy (Hall and Schneyer 1964;Wilborn and Schneyer 1970;Hand and Ho 1981;Scott et al 1990;Scott and Gunn 1991). To clarify whether apoptosis and proliferation of acinar cells participate in atrophy of rat parotid glands induced by liquid diet, rats were fed a liquid diet and compared to pellet-fed controls.

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Involvement of apoptosis and proliferation of acinar cells in atrophy of rat parotid glands induced by liquid diet

et al. 2012

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Parotid glands of experimental animals fed a liquid diet are reported to show atrophy (Hall and Schneyer 1964;Wilborn and Schneyer 1970;Hand and Ho 1981;Scott et al. 1990;Scott and Gunn 1991). To clarify whether apoptosis and proliferation of acinar cells participate in atrophy of rat parotid glands induced by liquid diet, rats were fed a liquid diet and compared to pellet-fed controls. Parotid glands were removed at 3, 7, 14 or 21 days, weighed, and examined using transmission electron microscopy (TEM), and studied immunohistochemically for cleaved-caspase-3 (Casp-3), a marker of apoptotic cells, and 5-bromo-2`-deoxyuridine (BrdU), a marker for proliferating cells. Body weights of experimental rats fed liquid diets were not significantly different from controls fed pellet diets; however weights of experimental parotid glands were smaller than those of controls. In the experimental parotid glands, structures like apoptotic bodies were histologically observed in acini at each time point; more Casp-3-positive acinar cells were identified in experimental parotid glands than in the controls on days 3, 7, and 14. Experimental glands showed fewer BrdU-positive acinar cells at each time point. TEM confirmed typical apoptotic acinar cells in the atrophic glands. These findings suggest that increased acinar cell apoptosis and reduced acinar cell proliferation occur in atrophic parotid glands of rats fed a liquid diet.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…

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mentioning

confidence: 99%

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Involvement of apoptosis and proliferation of acinar cells in atrophy of rat parotid glands induced by liquid diet

et al. 2012

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“…To investigate these influences, an experimental model of feeding a liquid diet to animals is widely used. Parotid glands from liquid-fed animals become atrophic, acinar cells are reduced in size (Hand and Schneyer, 1964;Wilborn and Schneyer, 1970;Hand and Ho, 1981;Scott and Gunn, 1991;Takahashi et al, 2012) and acinar cell numbers decrease in the atrophic parotid glands (Johnson, 1982;Scott et al, 1990;Takahashi et al, 2012). Despite an increasing accumulation of knowledge regarding parotid glands of liquid-fed animals, few reports have investigated other salivary glands such as the submandibular and sublingual glands.…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical and ultrastructural investigation of acinar cells in submandibular and sublingual glands of rats fed a liquid diet

et al. 2014

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Running Title: Submandibular and sublingual gland of rat fed liquid diet Keywords: submandibular gland, sublingual gland, liquid diet, cell proliferation, apoptosis, ABSTRACTIn atrophic parotid glands induced by liquid diet, acinar cell apoptosis is increased while proliferative activity is reduced. This study aimed to clarify how liquid diet affects submandibular and sublingual glands, including acinar cell apoptosis and proliferation.Seven-week-old male Wistar rats were fed either a liquid (experimental group) or pellet diet (control group) from 3 to 21 days, respectively. Submandibular and sublingual glands were weighed and examined histologically, ultrastructurally, and immunohistochemically using antibodies to cleaved caspase-3 (Casp-3) and 5-bromo-2'-deoxyuridine (BrdU). Weights of submandibular and sublingual gland from the experimental group were not significantly different from controls at any time point. Histological and ultrastructural characteristics of experimental acinar cells in both glands were normal. Acinar cells in control and experimental submandibular glands were positively stained with periodic acid Schiff (PAS) and weakly stained by alcian blue (AB). In control and experimental sublingual glands, mucous acinar cells were PAS-positive and strongly AB-positive. Although Casp-3-and BrdU-positive acinar cells were identified in both glands in the experimental group, their labeling indices were not significantly different from controls. In conclusion, liquid diet in rats does not induce atrophic alterations to acinar cells, including apoptosis and proliferative activity in submandibular and sublingual glands.3

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Ultrastructure of the principal and accessory submandibular glands of the common vampire bat

1990

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The principal and accessory submandibular glands of the common vampire bat, Desmodus rotundus, were examined by electron microscopy. The secretory endpieces of the principal gland consist of serous tubules capped at their blind ends by mucous acini. The substructure of the mucous droplets and of the serous granules varies according to the mode of specimen preparation. With ferrocyanide-reduced osmium postfixation, the mucous droplets are moderately dense and homogeneous; the serous granules often have a polygonal outline and their matrix shows clefts in which bundles of wavy filaments may be present. With conventional osmium postfixation, the mucous droplets have a finely fibrillogranular matrix; the serous granules are homogeneously dense. Mucous cells additionally contain many small, dense granules that may be small peroxisomes, as well as aggregates of 10-nm cytofilaments. Intercalated duct cells are relatively unspecialized. Striated ducts are characterized by highly folded basal membranes and vertically oriented mitochondria. Luminal surfaces of all of the secretory and duct cells have numerous microvilli, culminating in a brush borderlike affair in the striated ducts. The accessory gland has secretory endpieces consisting of mucous acini with small mucous demilunes. The acinar mucous droplets contain a large dense region; the lucent portion has punctate densities. Demilune mucous droplets lack a dense region and consist of a light matrix in which fine fibrillogranular material is suspended. A ring of junctional cells, identifiable by their complex secretory granules, separates the mucous acini from the intercalated ducts. The intercalated ducts lack specialized structure. Striated ducts resemble their counterparts in the principal gland. As in the principal gland, all luminal surfaces are covered by an array of microvilli. At least some of the features of the principal and accessory submandibular glands of the vampire bat may be structural adaptations to the exigencies posed by the exclusively sanguivorous diet of these animals and its attendant extremely high intake of sodium chloride.

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Liquid-diet-induced alterations of rat parotid acinar cells studied by electron microscopy and enzyme cytochemistry

Cited by 52 publications

References 37 publications

Involvement of apoptosis and proliferation of acinar cells in atrophy of rat parotid glands induced by liquid diet

Involvement of apoptosis and proliferation of acinar cells in atrophy of rat parotid glands induced by liquid diet

Immunohistochemical and ultrastructural investigation of acinar cells in submandibular and sublingual glands of rats fed a liquid diet

Ultrastructure of the principal and accessory submandibular glands of the common vampire bat

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