Liquid-Liquid Phase Separation of the DEAD-Box Cyanobacterial RNA Helicase Redox (CrhR) into Dynamic Membraneless Organelles in
            <i>Synechocystis</i>
            sp. Strain PCC 6803

Whitman, Brendan T; Wang, Yixiong; Murray, Cameron R A; Glover, Mark; Owttrim, George W.

doi:10.1128/aem.00015-23

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2023

2024

Publication Types

Select...

Article4

Preprint1

Relationship

Self Cite0

Independent5

Authors

Journals

Cited by 6 publications

References 79 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Proteomic composition of eukaryotic and bacterial RNA decay condensates suggests convergent evolution

Rathnayaka-Mudiyanselage,

Nandana,

Schrader

2024

Current Opinion in Microbiology

View full text Add to dashboard Cite

Proteomic composition of eukaryotic and bacterial RNA decay condensates suggests convergent evolution

Rathnayaka-Mudiyanselage,

Nandana,

Schrader

2024

Current Opinion in Microbiology

View full text Add to dashboard Cite

The molecular basis for cellular function of intrinsically disordered protein regions

Holehouse,

Kragelund

2023

Nat Rev Mol Cell Biol

153

View full text Add to dashboard Cite

R-DeeP/TripepSVM identifies the RNA-binding OB-fold-like protein PatR as regulator of heterocyst patterning

Brenes-Álvarez,

Ropp,

Papagiannidis

et al. 2024

Nucleic Acids Research

View full text Add to dashboard Cite

RNA-binding proteins (RBPs) are central components of gene regulatory networks. The differentiation of heterocysts in filamentous cyanobacteria is an example of cell differentiation in prokaryotes. Although multiple non-coding transcripts are involved in this process, no RBPs have been implicated thus far. Here we used quantitative mass spectrometry to analyze the differential fractionation of RNA–protein complexes after RNase treatment in density gradients yielding 333 RNA-associated proteins, while a bioinformatic prediction yielded 311 RBP candidates in Nostoc sp. PCC 7120. We validated in vivo the RNA-binding capacity of six RBP candidates. Some participate in essential physiological aspects, such as photosynthesis (Alr2890), thylakoid biogenesis (Vipp1) or heterocyst differentiation (PrpA, PatU3), but their association with RNA was unknown. Validated RBPs Asl3888 and Alr1700 were not previously characterized. Alr1700 is an RBP with two oligonucleotide/oligosaccharide-binding (OB)-fold-like domains that is differentially expressed in heterocysts and interacts with non-coding regulatory RNAs. Deletion of alr1700 led to complete deregulation of the cell differentiation process, a striking increase in the number of heterocyst-like cells, and was ultimately lethal in the absence of combined nitrogen. These observations characterize this RBP as a master regulator of the heterocyst patterning and differentiation process, leading us to rename Alr1700 to PatR.

show abstract

Liquid-Liquid Phase Separation of the DEAD-Box Cyanobacterial RNA Helicase Redox (CrhR) into Dynamic Membraneless Organelles in Synechocystis sp. Strain PCC 6803

Cited by 6 publications

References 79 publications

Proteomic composition of eukaryotic and bacterial RNA decay condensates suggests convergent evolution

Proteomic composition of eukaryotic and bacterial RNA decay condensates suggests convergent evolution

The molecular basis for cellular function of intrinsically disordered protein regions

R-DeeP/TripepSVM identifies the RNA-binding OB-fold-like protein PatR as regulator of heterocyst patterning

Contact Info

Product

Resources

About