Liquid Nutrient Medium for Submerged Cultivation of Tularemia Microbe

Volokh, O. A.; Антонычева, М. В.; Авдеева, Н. Г.; Kuznetsova, E. M.; Холматов, К. И.; Бибиков, Д. Н.; Никифоров, А. К.

doi:10.21055/0370-1069-2017-2-81-83

Cited by 4 publications

(4 citation statements)

References 2 publications

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“…сконструированная питательная среда расширяет спектр питательных сред для накопления бактериальной массы F. tularensis в условиях глубинного культивирования. на разработанную питательную среду был получен патент на изобретение [1].…”

Section: материалы и методыunclassified

Enhancement of the Technology for Live Tularemia Vaccine Production

Volokh¹,

Комиссаров²,

Антонычева³

et al. 2016

Problemy Osobo Opasnykh Infektsii

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вакцинопрофилактика является одним из наиболее развитых направлений противоэпидемической защиты. в рФ для специфической профилактики туляремии применяют туляремийную живую сухую вакцину, которая в 2011 г. приказом министерства здравоохранения и социального развития рФ (№ 51н от 31 января 2011 г.) включена в национальный календарь профилактических прививок по эпидемическим показаниям. в этой связи становится очевидным необходи-Биотехнология, иММунология

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Section: материалы и методыunclassified

Enhancement of the Technology for Live Tularemia Vaccine Production

Volokh¹,

Комиссаров²,

Антонычева³

et al. 2016

Problemy Osobo Opasnykh Infektsii

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“…Наряду с выпуском этих лекарственных средств ведутся активные научные исследования по направлению малоотходных технологий в иммунобиологическом производстве. В настоящее время в качестве основы для питательных сред активно используется фибрин -отход при производстве АИГ [1,2]; разработана технология регенерации спиртовых отходов [3]; из отходов производства специфических компонентов холерной вакцины получены биологически активные вещества [4].…”

Section: Introductionunclassified

Ecological safety and prospects of development of low-waste technologies in the biotechnology industry

Vol’nikov¹,

Ul’yanov²,

Salikhov³

et al. 2021

CBE

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Environmental pollution with industrial waste is an urgent problem today. A special place in the list of pollutants belongs to waste from biotechnological enterprises and industries, whose activities are related to the production of various drugs. Russian Research Anti-Plague Institute «Microbe» is the only manufacturer of unique immunobiological drugs in the Russian Federation – bivalent chemical cholera vaccine and rabies immunoglobulin from horse blood serum (AIG). At present, the institute actively uses fibrin as a basis for nutrient media – a waste in the production of AIG; a technology for the regeneration of alcohol waste has been developed; biologically active substances were obtained from the production waste of specific components of the cholera vaccine. The aim of the work was to assess the prospects of using waste products from the production of specific components of cholera vaccine (cholerogen-toxoid – X-AT, and O-antigen – O-AG) – formalized detoxified microbial-free filtrate (FMF), as a nutrient medium for the cultivation of industrial strains of microorganisms. It has been shown that the best methods for reducing formalin concentration are autoclaving and chemical neutralization with aqueous ammonia. During low-volume cultivation of Vibrio cholerae 569B and V. cholerae M-41 strains on all variants of experimental media based on PBP, an increase in biomass was noted. The production of Vibrio cholerae antigens at a level comparable to that of growing on a control nutrient medium was recorded in a medium variant based on O-AG production waste. The use of FMF as a nutrient medium in the future will reduce the volume of waste generated and reduce the load on the treatment facilities of the Institute, which will increase the environmental safety of production.

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“…одной из перспективных производственных питательных сред является среда на основе панкреатического гидролизата фибрина (ФгФ), полученного из отхода производства гетерологичного антирабического иммуноглобулина, согласно патенту RU 2425866 C1 [3]. в институте «микроб» разработаны и апробированы питательные среды на основе ФгФ для глубинного культивирования Vibrio cholerae и Francisella tularensis [4,5]. использование ФгФ в производственной практике получения штаммов-адсорбентов Y. pseudotuberculosis позволяет сократить расходы при получении клеточной массы, что в конечном итоге может значимо отразиться на себестоимости препарата.…”

unclassified

Optimization of the Conditions for Cultivation of Yersinia pseudotuberculosis in the Process of Obtaining Cell Mass

Salikhov

Borisova

Авдеева

et al. 2022

Problemy Osobo Opasnykh Infektsii

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Objective of the study was to optimize the conditions of submerged recurrent cultivation and the composition of the nutrient medium for obtaining the cell mass of Yersinia pseudotuberculosis strains, used as an adsorbent in the preparation of diagnostic plague immunoglobulins. Materials and methods. We utilized Y. pseudotuberculosis adsorbent strains 6; 31; 68; 69; and 70 belonging to V, I, III, IV, and V serotypes, respectively, received from the State Collection of Pathogenic Bacteria at the premises of the RusRAPI “Microbe”. The cultivation process was carried out on an incubator-shaker, laboratory and pilot fermenters with variation of the process parameters, different options for feeding and nutrient media. Results and discussion. In the course of work, the optimal parameters of recurring submerged cultivation have been established. It was found that the highest biomass yield is provided by a combination of a nutrient medium – a carbon substrate in the form of a broth, based on an enzymatic fibrin hydrolysate, with the addition of galactose as a substrate feeding. Thereat, the morphology and immunochemical properties of microbial cells obtained through modified preparation process do not differ from those produced in the control run. The optimization of the parameters for Y. pseudotuberculosis cell mass cultivation with subsequent upscaling has been performed.

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Liquid Nutrient Medium for Submerged Cultivation of Tularemia Microbe

Cited by 4 publications

References 2 publications

Enhancement of the Technology for Live Tularemia Vaccine Production

Enhancement of the Technology for Live Tularemia Vaccine Production

Ecological safety and prospects of development of low-waste technologies in the biotechnology industry

Optimization of the Conditions for Cultivation of Yersinia pseudotuberculosis in the Process of Obtaining Cell Mass

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