Listeria monocytogenes induced dysbiosis in snails and rebiosis achieved by administration of the gut commensal Lactobacillus plantarum Sgs14 strain

Dushku, Esmeralda; Kotzamanidis, Christos; Avgousti, Kalodoti; Ζdragas, Αntonios; Vafeas, George; Giantzi, Virginia; Στάικου, Αλεξάνδρα; Yiangou, Minas

doi:10.1016/j.fsi.2020.04.041

Cited by 10 publications

(7 citation statements)

References 41 publications

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“…Dushku et al [44] reported that the biofilm formation capability of L. monocytogenes was associated with cell surface properties, such as hydrophobicity and auto-aggregation, which were essential for its increased tolerance to gut barriers, survival in the host, and hypervirulence activity. Hydrophobicity influences bacterial adhesion, proliferation, and distribution.…”

Section: Discussionmentioning

confidence: 99%

“…monocytogenes biofilms formed in food processing environments are resistant to sanitizers and hard to eradicate, which poses a serious food safety concern. Dushku et al [ 44 ] reported that the biofilm formation capability of L . monocytogenes was associated with cell surface properties, such as hydrophobicity and auto-aggregation, which were essential for its increased tolerance to gut barriers, survival in the host, and hypervirulence activity.…”

Section: Discussionmentioning

confidence: 99%

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Antimicrobial Activity of Ohelo Berry (Vaccinium calycinum) Juice against Listeria monocytogenes and Its Potential for Milk Preservation

Liu

Nakamoto

et al. 2022

Microorganisms

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Listeria monocytogenes is a foodborne pathogen and causes illnesses with a high mortality rate in susceptible populations. Several dairy-related outbreaks have been attributed to contamination by L. monocytogenes, which requires antimicrobial interventions to enhance the safety of these products. This study aimed to determine the antimicrobial activity of the ohelo berry (Vaccinium calycinum), a Hawaiian wild relative of cranberry, against L. monocytogenes in culture media and milk products. The effect of ohelo berry juice at its sub-inhibitory concentrations on the physicochemical properties, biofilm formation, and gene expression of L. monocytogenes was also investigated. The minimum inhibitory concentration of ohelo berry juice against L. monocytogenes was 12.5%. The sub-inhibitory concentration of ohelo berry juice (6.25%) significantly increased the auto-aggregation and decreased the hydrophobicity, swimming motility, swarming motility, and biofilm formation capability of L. monocytogenes. The relative expression of genes for motility (flaA), biofilm formation and disinfectant resistance (sigB), invasion (iap), listeriolysin (hly), and phospholipase (plcA) was significantly downregulated in L. monocytogenes treated by the 6.25% juice. L. monocytogenes was significantly inhibited in whole and skim milk supplemented with 50% ohelo berry juice, regardless of the fat content. These findings highlight the potential of ohelo berry as a natural preservative and functional food to prevent L. monocytogenes infection.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Antimicrobial Activity of Ohelo Berry (Vaccinium calycinum) Juice against Listeria monocytogenes and Its Potential for Milk Preservation

Liu

Nakamoto

et al. 2022

Microorganisms

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“…This may have helped to digest gastric mucus, thus facilitating the invasion of gastric epithelial cells by Helicobacter sp. L. monocytogenes was reported to induce dysbiosis in snails ( Dushku et al, 2020 ), and H. pylori is thought to be a new fish-borne pathogen of tilapia ( Abdel-Moein et al, 2015 ). Thus, L. monocytogenes and H. pylori may attack the gut of crayfish.…”

Section: Discussionmentioning

confidence: 99%

Metagenomics Analysis Reveals Compositional and Functional Differences in the Gut Microbiota of Red Swamp Crayfish, Procambarus clarkii, Grown on Two Different Culture Environments

et al. 2021

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The structure and function of intestinal microorganisms are closely related to host metabolism, development, physiology, and health. The red swamp crayfish, Procambarus clarkii, is an important farmed aquatic species in China, which is grown in aquaculture ponds and rice paddy fields. Since these are two distinct cultivation environments with important differences in nutrient input and ecological community composition, we hypothesized that they may have different effects on the gut microbiota of the crayfish. Here, we sought to examine this hypothesis. To that aim, metagenomics analyses were applied to unveil the taxonomic composition and functional diversity of the microbiota in the intestines of red swamp crayfish grown in aquaculture ponds and rice-crayfish cultivation environments. The results showed that Firmicutes and Proteobacteria were the two most abundant microbial components. In addition, the relative abundance of bacterial and archaeal communities, but not that of fungal and viral communities, significantly differed between the two environments. The abundance of genes involved in pathways related to genetic information processing and human diseases was lower in the guts of red swamp crayfish grown in rice-crayfish cultivation environments. In particular, the abundance of two gene sets, K13730 and K08303, which are related to epithelial cell invasion by Listeria monocytogenes and Helicobacter pylori, respectively, decreased in this culture environment. In addition, the samples from rice-crayfish cultivation environments tended to have lower relative abundance of glycosyltransferases (GTs), which were the most abundant carbohydrate-active enzymes in the samples from both groups, higher abundance of glycoside hydrolases, and lower abundance of GT2.

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“…It has been recently reported that isolated strains of the genera Lactobacillus from the intestinal tract of the terrestrial gastropods Cornu aspersum (O.F. Müller, 1774) act as probiotics by enhancing their immune system when administered together with the diet [ 25 , 26 ]. A total of 48 candidate probiotic strains of Lactobacillus and 15 potential pathogen strains of Listeria monocytogenes were isolated and investigated at a functional, phenotypic, and genetic level [ 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

“…Müller, 1774) act as probiotics by enhancing their immune system when administered together with the diet [ 25 , 26 ]. A total of 48 candidate probiotic strains of Lactobacillus and 15 potential pathogen strains of Listeria monocytogenes were isolated and investigated at a functional, phenotypic, and genetic level [ 25 , 26 ]. The aim of the present work was to further investigate whether the use of probiotics exerts beneficial physiological effects associated with protein synthesis, mitochondrial metabolism, apoptosis, and stress responses on immature individuals of the land snail species C. aspersum .…”

Section: Introductionmentioning

confidence: 99%

Evidence for Beneficial Physiological Responses of the Land Snail Cornu aspersum to Probiotics’ (Lactobacillus plantarum) Dietary Intervention

Efstratiou,

Feidantsis,

Makri

et al. 2024

Animals

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A large variety of microorganisms ingested with food constitute animals’ intestinal microflora, enhancing and maintaining the homeostasis of the host. Rations enriched with probiotics are a method recommended to prevent undesirable conditions. To date, research has been limited to farmed animals and reared fish, creating a knowledge gap concerning the effect of probiotics on the growth rate, physiological responses, and energy metabolism of invertebrates such as the land snail Cornu aspersum. Herein, juvenile snails (26.23 ± 0.5 mm shell diameter and 8.23 ± 1.02 g body weight) were fed L. plantarum probiotic-enriched rations in two different proportions (1.25 mg and 2.5 mg), and their growth rate was monitored for three months. Additionally, the RNA/DNA and Bax/Bcl-2 ratios, HSP gene expression and protein levels, and ND2 expression, were measured in the hepatopancreas, digestive tract, and mantle. Although the snails’ growth rate was not affected, the RNA/DNA ratio presented an increase in various tissues, indicating an intense physiological response. Also, probiotic administration demonstrated low levels of the Bax/Bcl-2 ratio. HSP levels were higher in the presence of probiotics, probably signaling an attempt by the animal to face potentially stressful situations. Finally, ND2 expression levels in the hepatopancreas indicate intense metabolic and antioxidant activity.

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Listeria monocytogenes induced dysbiosis in snails and rebiosis achieved by administration of the gut commensal Lactobacillus plantarum Sgs14 strain

Cited by 10 publications

References 41 publications

Antimicrobial Activity of Ohelo Berry (Vaccinium calycinum) Juice against Listeria monocytogenes and Its Potential for Milk Preservation

Antimicrobial Activity of Ohelo Berry (Vaccinium calycinum) Juice against Listeria monocytogenes and Its Potential for Milk Preservation

Metagenomics Analysis Reveals Compositional and Functional Differences in the Gut Microbiota of Red Swamp Crayfish, Procambarus clarkii, Grown on Two Different Culture Environments

Evidence for Beneficial Physiological Responses of the Land Snail Cornu aspersum to Probiotics’ (Lactobacillus plantarum) Dietary Intervention

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