Listeria monocytogenes infection in beta 2 microglobulin-deficient mice

Roberts, Alan D.; Ordway, Diane; Orme, Ian M.

doi:10.1128/iai.61.3.1113-1116.1993

Cited by 88 publications

(24 citation statements)

References 17 publications

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“…Our data, in accordance with recently published data from two other investigators, 28,29 demonstrate that b 2 M7/7 knock-out mice have an impaired ability to clear promptly an infection with L. monocytogenes. Additionally, our data show several novel findings regarding the function of class II-restricted CTL as compared to class I-restricted CTL during a bacterial infection.…”

Section: Discussionsupporting

confidence: 93%

CD4⁺ cytolytic effectors are inefficient in the clearance of Listeria monocytogenes

et al. 1996

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SUMMARYCytotoxic T lymphocytes (CTL) recognize and lyse target cells through the interaction of the T-cell receptor complex with the class I or class II major histocompatibility complex (MHC). The production of class I-restricted CTL has been shown to be critical to the elimination of specific pathogens including Listeria monocytogenes. However, the function of class II-restricted CTL in the clearance of intracellular pathogens is poorly understood. H-2 b b2 -microglobulin-deficient mice (b 2 Mÿ/ÿ) are not able to produce CD8 CTL in response to infection with L. monocytogenes. We used this model to evaluate the efficacy of class II-restricted CTL, in the absence of a class I-restricted response, during a primary infection with L. monocytogenes. We demonstrate that, despite their effectiveness in adoptive transfer of protection, Listeria-specific CD4 class II-restricted cytotoxic lymphocytes are ineffective in decreasing titres of L. monocytogenes in the spleen after an established infection. In b 2 Mÿ/ÿ mice, persistence of L. monocytogenes in the spleen was found preferentially in class II-negative cells. Surprisingly, class Irestricted CTL from C57BL/6 mice were capable of decreasing bacterial titres during an established infection even in the absence of detectable class I on the surface of cells from b 2 Mÿ/ÿ mice. These data strongly suggest that, in the absence of a class I-restricted response, pathogens that elicit a class IIrestricted cytotoxic response may escape prompt eradication by the immune system.

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Section: Discussionsupporting

confidence: 93%

CD4⁺ cytolytic effectors are inefficient in the clearance of Listeria monocytogenes

et al. 1996

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“…Mice depleted of CD8 + , but not CD4 + , T cells by the administration of subset-specific monoclonal antibody (mAb) exhibit a decreased capacity to resolve both primary and secondary listerial infections (8,19,20). Similarly, MHC-deficient (knock-out) mice lacking functional CD8 + T cells are far more susceptible to listerial infections than mice lacking functional CD4 + T cells (11,21). In contrast to CD8 + T-cell-deficient animals, mice adoptively immunized with a CD8-enriched T-cell population exhibit a marked increase in resistance to Listeria (9,10).…”

Section: Mhc Class I-restricted Cd8 + T-cell-response To Listeriamentioning

confidence: 99%

CD8+ T-cell-mediated response to Listeria monocytogenes taken up in the liver and replicating within hepatocytes

Gregory

Liu

2000

Immunological Reviews

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Like most other organisms that enter the bloodstream, the bulk of Listeria monocytogenes injected i.v. into mice is taken up by the liver. Listeriae not killed rapidly by infiltrating neutrophils are internalized by hepatocytes which constitute the principal site of intracellular replication in the liver. CD8+ T cells play a critical role in eliminating infected hepatocytes and resolving listerial infections of the liver; the specific mechanisms involved are not understood fully. Here, we review recent data implicating the cytolytic activities expressed by MHC class Ia- and class Ib-restricted CD8+ T cells in host resistance to Listeria. Evidence demonstrating the perforin- and/or Fas ligand-dependent induction of caspase activity and the resultant apoptosis of Listeria-infected hepatocytes is discussed.

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“…Both CD4 + (helper, MHC class II restricted) and CD8 + (cytotoxic, MHC class I restricted) T cell subpopulations have been implicated [20]. Experimental evidence indicates, however, that CD8 + T cells play the predominant role in mediating protective immunity [21][22][23][24]. The ability of the recombinant L.inn::vgc to induce T-cell mediated immunity as a prerequisite for protective immunity was analyzed.…”

Section: Induction Of T Cell-mediated Immunity By the Recombinant LImentioning

confidence: 99%

Protective Immunity to Listeria Monocytogenes Infection Mediated by Recombinant Listeria innocua Harboring the VGC Locus

et al. 2012

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In this study we propose a novel bacterial vaccine strategy where non-pathogenic bacteria are complemented with traits desirable for the induction of protective immunity. To illustrate the proof of principle of this novel vaccination strategy, we use the model organism of intracellular immunity Listeria. We introduced a, low copy number BAC-plasmid harbouring the virulence gene cluster (vgc) of L. monocytogenes (Lm) into the non-pathogenic L. innocua (L.inn) strain and examined for its ability to induce protective cellular immunity. The resulting strain (L.inn::vgc) was attenuated for virulence in vivo and showed a strongly reduced host detrimental inflammatory response compared to Lm. Like Lm, L.inn::vgc induced the production of Type I Interferon's and protection was mediated by Listeria-specific CD8+ T cells. Rational vaccine design whereby avirulent strains are equipped with the capabilities to induce protection but lack detrimental inflammatory effects offer great promise towards future studies using non-pathogenic bacteria as vectors for vaccination.

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Listeria monocytogenes infection in beta 2 microglobulin-deficient mice

Cited by 88 publications

References 17 publications

CD4⁺ cytolytic effectors are inefficient in the clearance of Listeria monocytogenes

CD4⁺ cytolytic effectors are inefficient in the clearance of Listeria monocytogenes

CD8+ T-cell-mediated response to Listeria monocytogenes taken up in the liver and replicating within hepatocytes

Protective Immunity to Listeria Monocytogenes Infection Mediated by Recombinant Listeria innocua Harboring the VGC Locus

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Listeria monocytogenes infection in beta 2 microglobulin-deficient mice

Cited by 88 publications

References 17 publications

CD4+ cytolytic effectors are inefficient in the clearance of Listeria monocytogenes

CD4+ cytolytic effectors are inefficient in the clearance of Listeria monocytogenes

CD8+ T-cell-mediated response to Listeria monocytogenes taken up in the liver and replicating within hepatocytes

Protective Immunity to Listeria Monocytogenes Infection Mediated by Recombinant Listeria innocua Harboring the VGC Locus

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Product

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CD4⁺ cytolytic effectors are inefficient in the clearance of Listeria monocytogenes

CD4⁺ cytolytic effectors are inefficient in the clearance of Listeria monocytogenes