Lithospermum officinale callus produces shikalkin

Haghbeen, Kamahldin; Mozaffarian, Valiollah; Ghaffari, F.; Pourazeezi, Elahe; Saraji, Mohammad; Joupari, Morteza Daliri

doi:10.2478/s11756-006-0077-x

Cited by 12 publications

(8 citation statements)

References 26 publications

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“…Only a few in vitro processes, based on plant cell cultures, are already established in the cosmetic industry. Examples for such commercially available products are Shikonine as cosmetic pigment (Fujita and Tabata 1986;Payne et al 1991) (produced from Lithospermum erythrorhizon by Mitsui Petrochemical Industries, Japan), Arbutin as whitening ingredient (Misawa 1994;Yokoyama and Yanaigi (1991) (produced from Catharanthus roseus by Mitsui Petrochemical Industries, Japan) or Carthamin as cosmetic pigment (Yamamoto et al 2002;Haghbeen 2006;Ekiert 2004;Oda 2005) (produced from Carthamus tinctorius by Kibun, Japan). Nevertheless, the use of plant cell culture or their metabolite derived cosmetic active ingredients is very rare and in its infant stage.…”

Section: Biotechnology and Cosmeticsmentioning

confidence: 98%

Potential of plant cells in culture for cosmetic application

2007

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Plants and plant derived ingredients are common and of major importance in the fields of pharmacy, food and cosmetics. The cosmetic industry is a fast moving market. Products have short lifecycles and the industry has to come up with innovative products constantly. Most cosmetic products and their applications are defined by active ingredients. These active ingredients may derive from either synthetic sources or from plant sources. Beside this, no other origin like human or animal are accepted or allowed in cosmetics nor are genetically modified plant sources. The whole cosmetic research and development society is therefore desperately seeking for new innovative plant ingredients for cosmetic application. Unfortunately, new plant derived ingredients are limited because several plants of cosmetic interest are not to be used due to following facts: the plants contain toxic metabolites, the plants grow too slow and a seasonal harvesting is not possible, the concentration of plant constituents differ from harvest to harvest or the plant is endangered and not allowed to harvest. With the plant cell culture technology we bring complete new aspects in the development of novel cosmetic plant derived actives. Due to all these findings, we decided to risk the step into plant cell culture derived cosmetic active ingredient production. This article describes the successful establishment of an apple suspension culture producing a high yield of biomass, cultured in disposable, middle-scale bioreactors. The use of a bioactive extract out of these cells for cosmetic application and the efficacy of this extract on mammalian stem cells is also outlined in this article. To obtain a suitable cosmetic product we used the high pressure homogenization technique to decompose the plant cells and release all the beneficial constituents while encapsulating these components at the same time in liquid Nanoparticles. With the plant cell culture technology we bring complete new aspects in the development of novel cosmetic plants derived actives.

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Section: Biotechnology and Cosmeticsmentioning

confidence: 98%

Potential of plant cells in culture for cosmetic application

2007

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“…The callus sample was taken from the solid medium, was removed from cells and dried at 37°C for 48 h. The callus was powdered and subjected to extraction by radical-free tetrahydrofuran (THF) (11). Extraction was carried out at room temperature by stirring the powder in THF for 10 min.…”

Section: Pigment Extraction and Measurementsmentioning

confidence: 99%

“…Extraction was carried out at room temperature by stirring the powder in THF for 10 min. Concentration of the pigment in THF was determined spectrophotometrically by reading the absorbance at 520 nm (11). A calibration plot, obtained for the shikalkin pigment extracted from the natural root of Iranian A. euchroma, was used in calculations.…”

Section: Pigment Extraction and Measurementsmentioning

confidence: 99%

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Enhancement of Shikalkin Production in Arnebia euchroma Callus by a Fungal Elicitor, Rhizoctonia solani

2015

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Background: There is a growing demand for mass production of shikalkin (a natural pigment consisted of shikonin and alkannin) due to its increasing applications in cosmetics, pharmaceutical and nutrition industries. The root of Iranian Arnebia euchroma produces shikalkin. The promising capability of this plant for shikalkin production has already been demonstrated in cell culture studies. Objectives: Elicitation effect of Rhizoctonia solani (R. solani) in comparison with the effects of Cu 2+ , methyl jasmonate (MJ), and salicylic acid (SA) on the shikalkin production was investigated in A. euchroma callus. Materials and Methods:The calli from different origins (leaf, collar and root) were proliferated on a modified LinsmaierSkoog (mLS) medium and were subsequently transferred onto the pigment production medium containing various amounts of the desirable elicitor. Observations were quantified and the pigment production was precisely measured spectrophotometrically. Results: Pigment biosynthesis was induced on White medium containing IAA (1 μM) and kinetin (10 μM) in dark at 25°C. Use of R. solani increased the pigment production by 7 fold greater than normal White medium. Cu 2+ only doubled the shikalkin production. MJ and SA showed enhancing effects comparable to that of Cu 2+ . Discussions: It is assumed that upon binding of the polysaccharides of the fungal cells to the plant cell surface, a cascade of signaling is initiated that led to expression of genes involving in the biosynthesis of shikalkin.

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“…[6][7][8] Shikonin and alkanin are red and lipophilic pigments seen in most species. The other species of Arnebia such as nobilis, hispidissima, densiflora, and decumbens are found all over the world.…”

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confidence: 99%