Lithotrophic and Heterotrophic Bacteria in Deep Subsurface Sediments and Their Relation to Sediment Properties

Fredrickson, Jim K.; Garland, T.; Hicks, Ronald J.; Thomas, John; Li, S.W.; McFadden, K.M.

doi:10.1080/01490458909377849

Cited by 160 publications

(76 citation statements)

References 21 publications

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“…Differences were noted in the microbial populations based on lithologic characteristics (Balkvdl 1989;Fredrickson et al 1989;Phelps et al 1989b;Sinclair and Ghiorse 1989) and depth of the samples (Fredrickson et al 1988;Sinclair and Ghiorse 1989). The results of these studies suggest that subsurface rn~crobialpopulations play a significant role in diagenesis and subsurface geochemistry at SRS.…”

Section: Microbiologymentioning

confidence: 67%

“…Fredrickson et al (1989) found that sediment texture, primarily clay content, was the best predictor of bacterial populations, showing an inverse relationship. The bacterial populations decreased as clay content increased.…”

Section: Llthologic Influences On Microbial Populationsmentioning

confidence: 92%

“…Microbial Diversity Fredrickson et al (1989) quantified autotrophic and heterotrophic bacterial populations in SRS subsurface sediments from the P24, P28 and P29 boreholes (Figure 6-6). Autotrophlc bacteria populations were generally less dense than 103 per gram of dry sediment, with sulfur-oxidizing bacteria being the most frequently encountered group.…”

Section: Microbiologymentioning

confidence: 99%

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SRS Geology/Hydrogeology Environmental Information Document

Denham¹

1999

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Section: Microbiologymentioning

confidence: 67%

Section: Llthologic Influences On Microbial Populationsmentioning

confidence: 92%

Section: Microbiologymentioning

confidence: 99%

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SRS Geology/Hydrogeology Environmental Information Document

Denham¹

1999

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“…Absorbance readings of 0.100 or above, after subtraction of the medium blanks, were accepted as positive. The presence of sulfur oxidizers was sought by employing the medium of Fredrickson et al [4]; these MPN tubes were tested at approximately 4-week intervals after inoculation for acidity by spotting samples onto pH paper. The MPN calculations were performed by using the BASIC program of Koch [12].…”

Section: Mpn Determinationsmentioning

confidence: 99%

Viability and metabolic features of bacteria indigenous to a contaminated deep aquifer

McCarthy

Murray

1996

Microb Ecol

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The quantitation and characterization of indigenous bacteria of a deep aquifer, located in the southwestern United States and contaminated with halogenated aliphatic compounds, was undertaken. Water samples were obtained aseptically from depths of 45 to 151 m from four sites that ranged from 260 to 1,800 m in distance from the location of contaminant release. Sediment samples were also obtained from the proximal and distal sites for analyses. Results for aerobic and anaerobic colony-forming units were obtained on four agar media that were used to retrieve heterotrophs, oligotrophs, and pseudomonads. Most probable number estimates were obtained from a liquid medium favorable for oligotrophs. Representative isolates were tested against Biolog plates (Biolog, Inc., Hayward, Calif.) for patterns of carbon source utilization. Of 103 Gram-negative (GN) isolates, 48 could not be identified and the others were only tentatively identified via the Biolog database, and none of the 35 Gram-positive (GP) isolates were identifiable. However, the metabolic patterns were subjected to average cluster linkage analyses; the GN and GP bacteria were separable into eight and four groups, respectively. The oligotroph group comprised one-third of the GN and one-half of the GP isolates. The consensus carbon source utilization pattern for each group was determined and will be useful in future characterization of additional aquifer bacterial isolates. Although predominantly aerobic and oligotrophic, the microbial community of this aquifer was highly diverse with discernible viability and metabolic features of the microbiota distinctive to each of the four water and two sediment samples.

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“…The earth's deep subsurface (depth of 100 to 1,000 m), once considered a lifeless place, has now firmly been shown to be home to a host of bacteria (1,3,13,31). In such environments, bacteria often obtain only limited nutrients and energy and hence are capable of maintaining only very slow growth.…”

mentioning

confidence: 99%

Novel Group I Intron in the tRNA ^Leu (UAA) Gene of a γ-Proteobacterium Isolated from a Deep Subsurface Environment

2002

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A group I intron has been found to interrupt the anticodon loop of the tRNA Leu (UAA) gene in a bacterium belonging to the ␥-subdivision of Proteobacteria and isolated from a deep subsurface environment. The subsurface isolate SMCC D0715 was identified as belonging to the genus Pseudomonas. The group I intron from this isolate is the first to be reported for ␥-proteobacteria, and the first instance of a tRNA Leu (UAA) group I intron to be found in a group of bacteria other than cyanobacteria. The 231-nucleotide (nt) intron's sequence has group I conserved elements and folds into a bona fide group I secondary structure with canonical base-paired segments P1 to P9 and a paired region, P10. The D0715 intron possesses the 11-nt motif CCUACG . . . UAUGG in its P8 region, a feature not common in bacterial introns. To date, phylogenetic analysis has shown that bacterial introns form two distinct families, and their complex distribution suggests that both lateral transfer and common ancestry have taken part in the evolutionary history of these elements.

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Lithotrophic and Heterotrophic Bacteria in Deep Subsurface Sediments and Their Relation to Sediment Properties

Cited by 160 publications

References 21 publications

SRS Geology/Hydrogeology Environmental Information Document

SRS Geology/Hydrogeology Environmental Information Document

Viability and metabolic features of bacteria indigenous to a contaminated deep aquifer

Novel Group I Intron in the tRNA ^Leu (UAA) Gene of a γ-Proteobacterium Isolated from a Deep Subsurface Environment

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Lithotrophic and Heterotrophic Bacteria in Deep Subsurface Sediments and Their Relation to Sediment Properties

Cited by 160 publications

References 21 publications

SRS Geology/Hydrogeology Environmental Information Document

SRS Geology/Hydrogeology Environmental Information Document

Viability and metabolic features of bacteria indigenous to a contaminated deep aquifer

Novel Group I Intron in the tRNA Leu (UAA) Gene of a γ-Proteobacterium Isolated from a Deep Subsurface Environment

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Product

Resources

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Novel Group I Intron in the tRNA ^Leu (UAA) Gene of a γ-Proteobacterium Isolated from a Deep Subsurface Environment