“…Briefly, muscle tissues (0.1 g) were sampled from 9 surviving shrimps at 48, 72 and 96 h post infection and subjected to genomic DNA extraction. Absolute real-time quantitative PCR (qPCR) was performed to measure the quantities of WSSV genome copies using primers WSSV32678-F/WSSV32753-R and a TaqMan fluorogenic probe (Table 1) [30]. The PCR was performed in a 10 ml reaction mixture, containing 5 ml LightCycler ® 480 Probes Master (Roche, Germany), 2.5 ml DNA template, 1 ml of 10 mM primers mix, 0.02 ml of 100 mM TaqMan fluorogenic probe and 1.48 ml H 2 O.…”