Litorilituus lipolyticus sp. nov., isolated from intertidal sand of the Yellow Sea in China, and emended description of Colwellia asteriadis

Pontixanthobacter rizhaonensis sp. nov., a marine bacterium isolated from surface seawater of the Yellow Sea, and proposal of Pseudopontixanthobacter gen. nov., Pseudopontixanthobacter confluentis comb. nov. and Pseudopontixanthobacter sediminis comb. nov.

“…Cellular pigments were extracted in a mixture of acetone/methanol (7 : 2) and the absorption spectra were examined using a UV/VIS spectrophotometer V-550 (Jasco) as described by Biebl et al [25]. Activities of catalase, oxidase and DNase were determined as described previously [8]. Hydrolysis of starch (1 %, w/v), carboxymethyl cellulose (1 %, w/v), alginic acids (1 %, w/v), skimmed milk (1 %, w/v), gelatin (1 %, w/v), l -tyrosine (0.5 %, w/v), aesculin (0.01 %, w/v) and Tweens 20, 40, 60 and 80 (1 %, v/v) were detected on MA using the standard methods [26].…”

“…Cellular fatty acids of strain RZ02 T and the closely related type strains were determined as described previously [8] using cells grown in MB at 30 °C for 24 h when bacterial cultures reached the exponential phase of growth. The respiratory quinones were extracted as described by Komagata and Suzuki [27] and analysed by HPLC-MS. Polar lipids were extracted according to the procedures described by Komagata and Suzuki [27] and separated using two-dimensional thin-layer chromatography on Merck silica gel 60 F254 plates (10×10 cm).…”

“…The pH range for growth at pH 5.0-10.0 (at intervals of 0.5 pH units) was tested in MB supplemented with the following buffers: MES (pH 5.0-6.0, 50 mM), MOPS (pH 6.5-7.0, 50 mM), Tris (pH 7.5-8.5, 50 mM) and CHES (pH 9.0-10.0, 50 mM). Growth with 0, 0.5, 1-9 % (w/v) NaCl (at intervals of 1 %) was determined as described previously [8]. Growth under anaerobic conditions was detected on MA in an anaerobic jar supplemented with a disposable oxygen-absorbing and carbon dioxide-generating agent (Mitsubishi Gas Chemical) at 30 °C for 15 days.…”

“…Sensitivity to antibiotics of strain RZ02 T was tested by the disc diffusion method as described previously [8]. The discs (Hangzhou Tianhe Microbial Reagent Co.) contained the following antibiotics: erythromycin (15 µg), minocycline (30 µg), neomycin (30 µg), amikacin (30 µg), ceftriaxone (30 µg), cefuroxime (30 µg), ceftazidime (30 µg), cefuroxime (30 µg), cefazolin (30 µg), cephradine (30 µg), cephalexin (30 µg), piperacicillin (100 µg), carbenicillin (100 µg), ampicillin (10 µg), oxacillin (1 µg) and penicillin (10 U).…”

“…Genomic DNA was isolated and purified using a genomic DNA extraction kit (BioTeke) according to the instruction manual. The 16S rRNA gene was sequenced as described previously [8] and was compared with those available from the EzBioCloud database [9]. Multiple sequence alignment was conducted with the muscle programme integrated in mega software version X [10] and phylogenetic trees were reconstructed with the maximum-likelihood (ML) [11], neighbour-joining (NJ) [12] and maximum-parsimony (MP) [13] methods using mega X.…”