Live prey enrichment, with particular emphasis on HUFAs, as limiting factor in false percula clownfish (Amphiprion ocellaris, Pomacentridae) larval development and metamorphosis: Molecular and biochemical implications

Olivotto, Ike; Stefano, Michele Di; Rosetti, Silvestro; Cossignani, Lina; Pugnaloni, Armanda; Giantomassi, Federica; Carnevali, Oliana

doi:10.1016/j.cbpa.2011.02.004

Cited by 57 publications

(70 citation statements)

References 103 publications

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“…Other studies have also concluded that higher DHA content has a positive effect on fish performance during early stages (Mourente et al, 1993;Olivotto et al, 2011;Trushenski et al, 2012). On these stages there is a great energy demand because the rapid growth of fishes.…”

Section: Discussionmentioning

confidence: 94%

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Growth and survival of Hippocampus erectus (Perry, 1810) juveniles fed on Artemia with different HUFA levels

Vite-García

Simões

Arjona

et al. 2014

lajar

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ABSTRACT. Survival during first months after birth is one of the bottlenecks for consolidating the seahorse farming industry. In this work, Artemia metanauplii enriched with two highly unsaturated fatty acids (HUFA) rich commercial emulsions with different docosahexaenoic acid (DHA) levels (63% and 14% of total lipids), a vegetable oil with no DHA, and non-enriched Artemia as control, were used to feed 5-day-old juvenile Hippocampus erectus for 60 days. Enriched Artemia had similar levels of DHA (13% and 9%), despite great differences of DHA in the emulsions, with traces of DHA in non-enriched and vegetable oil enriched Artemia. More than 20% of DHA was found in 24 h starved juveniles fed both DHA-enriched treatments, similar to values in newly born juveniles, but those fed vegetable oil enriched Artemia or non-enriched Artemia had 5% of DHA. Total lipid and protein levels were similar in juveniles from the four treatments. The n-3/n-6 ratio was almost four-fold higher in seahorses fed DHA-enriched treatments compared to juveniles fed the nonenriched treatments. Survival of seahorses only partially reflected the DHA levels: it was lower in the vegetable oil treatment, similar in the seahorses fed Artemia with higher DHA and in the control treatment, and higher in seahorses fed the HUFA-enriched Artemia with lower DHA levels, although growth was similar in the two DHA-enriched Artemia treatments. Juvenile H. erectus seahorses perform better when they have at least 20% of DHA in their tissues, and these levels can be attained with no more than 14% of DHA in emulsions, eliminating the need for more expensive emulsions with higher DHA levels. Keywords: Hippocampus erectus, seahorse, DHA, growth, fatty acids, survival, aquaculture.Crecimiento y sobrevivencia de juveniles de Hippocampus erectus (Perry, 1810) alimentados con Artemia con diferentes niveles de HUFA RESUMEN. La sobrevivencia durante los primeros meses de vida es uno de los cuellos de botella para la consolidación de la industria del cultivo del caballito de mar (Hippocampus erectus). En este trabajo se utilizaron metanauplios enriquecidos con dos emulsiones comerciales, con diferentes niveles de ácido docosahexaenóico (DHA) (63% y 14%), con aceite vegetal sin DHA, y metanauplios sin enriquecimiento como control, para alimentar caballitos de mar de 5 días de vida durante 60 días. La Artemia enriquecida tuvo niveles similares de DHA (13% y 9%), a pesar de las grandes diferencias en las emulsiones, con trazas de DHA en la Artemia no enriquecida y la enriquecida con aceite vegetal. Se obtuvo más de 20% de DHA en juveniles, muestreados con 24 h de ayuno, de los tratamientos con enriquecimiento de DHA, con valores similares a los de caballitos recién nacidos, pero los caballitos que se alimentaron con Artemia enriquecida con aceite vegetal y con Artemia no enriquecida tuvieron 5% de DHA. Los lípidos y proteínas totales fueron similares en los caballitos de los cuatros tratamientos. La razón n-3/n-6 fue casi cuatro veces mayor en caballitos alimentados con los t...

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Section: Discussionmentioning

confidence: 94%

“…On these stages there is a great energy demand because the rapid growth of fishes. DHA promotes a better development and function of motocondria, and a higher production of enzymes and coenzimes with a central position in the metabolism (Olivotto et al, 2011;Yin & Tang, 2012).…”

Section: Discussionmentioning

confidence: 99%

Growth and survival of Hippocampus erectus (Perry, 1810) juveniles fed on Artemia with different HUFA levels

Vite-García

Simões

Arjona

et al. 2014

lajar

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“…To date, copepods are looked with great interest as a new and alternative food source (Holt ; Olivotto et al . ) especially because they represent the natural food for marine fish larvae in the wild.…”

Section: Discussionmentioning

confidence: 99%

“…; Olivotto et al . , ). In fact, deficiencies in these fatty acids can cause a general decrease in larval health, poor growth, low feed efficiency, anaemia that result in high mortality (Sargent et al .…”

Section: Introductionmentioning

confidence: 99%

The use of preserved copepods in sea bream small-scale culture: biometric, biochemical and molecular implications

et al. 2013

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Considering the well‐known problems arising from the use of rotifers and Artemia as live prey in larval rearing in terms of fatty acid deficiencies, the aim of this study was to evaluate a partial or complete replacement of traditional live prey with preserved copepods during the larviculture of gilthead sea bream (Sparus aurata). Sea bream larvae were randomly divided into 4 experimental groups in triplicates: group A larvae (control) fed rotifers followed by Artemia nauplii; group B fed a combined diet (50%) of rotifers–Artemia and preserved copepods; group C fed rotifers followed by preserved copepods; and group D fed preserved copepods solely. Survival and biometric data were analysed together with major molecular biomarkers involved in growth, lipid metabolism and appetite. Moreover, fatty acid content of prey and larvae was also analysed. At the end of 40 days treatment, a stress test, on the remaining larvae, was performed to evaluate the effects of different diets on stress response. Data obtained evidenced a positive effect of cofeeding preserved copepods during sea bream larviculture. Higher survival and growth were achieved in group B (fed combined diet) larvae respect to control. In addition, preserved copepods cofeeding was able to positively modulate genes involved in fish growth, lipid metabolism, stress response and appetite regulation.

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“…1999). A recent study by Olivotto et al (2011b) evaluated the effects of a standard and a HUFAs-enriched diet on A. ocellaris larvae. Clownfish have proven to be an excellent model for studying the importance of fatty acids in larval diets.…”

Section: Early Trialsmentioning

confidence: 99%

Clownfish

Olivotto

Geffroy

2017

Marine Ornamental Species Aquaculture

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Live prey enrichment, with particular emphasis on HUFAs, as limiting factor in false percula clownfish (Amphiprion ocellaris, Pomacentridae) larval development and metamorphosis: Molecular and biochemical implications

Cited by 57 publications

References 103 publications

Growth and survival of Hippocampus erectus (Perry, 1810) juveniles fed on Artemia with different HUFA levels

Growth and survival of Hippocampus erectus (Perry, 1810) juveniles fed on Artemia with different HUFA levels

The use of preserved copepods in sea bream small-scale culture: biometric, biochemical and molecular implications

Clownfish

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